Professor Viña has published over a hundred papers dealing with free radicals and antioxidants in several physiological and pathological situations. From the early 90´s, his laboratory has been interested in the role of free radicals in exercise, in particular exhaustive exercise. His team has worked in both physiological situations (1) and  pathological situations, in particular Type I Diabetes (2). 

Principal Investigators

José Viña Ph.D. M.D.

Staff Members

Mª Carmen Gómez Ph.D.

Researchers

Marco Romagnoli

Staff Members

Vladimir Essau Martínez Bello 

 

Research

Exercise causes an increase in the generation of free radicals by cells (Davies et al. 1982).  We showed these radicals to cause cellular damage only when exercise is exhaustive (Sastre et al. 1992).  Strenous exercise causes oxidation of glutathione, release of cytosolic enzymes (Sastre et al. 1992), and other signs of cell damage (Jackson, 1987). This is prevented by antioxidant administration (Sastre et al. 1992). 

 

We have recently reported that allopurinol protects against cell damage caused by exhaustive exercise both in humans (Gomez-Cabrera et al. 2003) and in experimental animals (Viña et al. 2000) thus highlighting the role of xanthine oxidase (XO) in free radical generation during exercise.   

 

 

          

 

 

However, free radicals not only cause damage but have a role in cell signalling (Reid et al. 1992; Jackson, 1999; Murrant & Reid, 2001). Moreover, the redox-sensitive transcription factor NF-κB is activated in exercise both in humans (Vider et al. 2001) and in animals (Hollander et al. 2001), leading to increased expression of superoxide dismutase (SOD), an enzyme involved in antioxidant defence (Ji et al. 2004).

 

 

 

Projects

 

“RED TEMÁTICA DE INVESTIGACIÓN COOPERATIVA EN ENVEJECIMIENTO Y FRAGILIDAD” (RETICEF) RD06/0013. Financed by FEDER funds  (Fondos Europeos de Desarrollo Regional). 

 

“MODIFICACIONES DE LA ACTIVIDAD DE GENES ASOCIADOS AL ENVEJECIMIENTO POR MANIPULACIONES NUTRICIONALES Y FISIOLÓGICAS. EFECTO SOBRE LA LONGEVIDAD EN MODELOS ANIMALES” BFU2007-65803. Financed by FEDER funds  (Fondos Europeos de Desarrollo Regional).

 

Selected Publications

1.- Sastre, J., Asensi, M., Gascó, E., Ferrero, J.A., Furukawa, T., Viña, J.  (1992) Exhaustive physical exercise causes oxidation of glutathione status in blood.  Prevention by antioxidant administration. American Journal of Physiology 263, R992 - R995.

2.- Viña, J., Sastre, J., Asensi, M., Packer, L. (1995) Assay of blood glutathione oxidation during physical exercise Methods in Enzymology  Vol. 251, Cap. 21, 237 – 243.

3.- Viña, J., Servera, E., Asensi, M., Sastre, J., Pallardó, F.V., Ferrero, J.A., García de la Asunción, J., Antón, V., Marín, J. (1996) Exercise causes blood glutathione oxidation in chronic obstructive pulmonary disease.  Prevention by oxygen therapy. Journal of Applied Physiology  81 (5), 2199 - 2202.

4.- Heunks, L.M., Vina, J., van Herwaarden, C.L., Folgering, H.T., Gimeno, A., Dekhuijzen, P.N. (1999) Xanthine oxidase is involved in exercise-induced oxidative stress in chronic obstructive pulmonary disease  Am J Physiol 277, R 1697 – 1704.

5.- Viña,J., Gimeno, A., Sastre, J., Desco, C., Asensi, M., Pallardó, F.V., Cuesta, A., Ferrero, J.A, Terada, L.S., Repine, J.E.  (2000)  Mechanism of Free Radical Production in Exhaustive Exercise in Humans and Rats; Role of Xanthine Oxidase and Protection by Allopurinol  IUBMB Life  Vol. 49, Issue 6, 539 – 544.  

6.-  Gómez-Cabrera  MC, Gimeno  A,  Lloret  A,  Miñana JB,  Márquez R and  Viña J (2000)  Deporte de alta competición y daño oxidativo: papel de los nutrientes antioxidantes. Antioxidantes y Calidad de vida. 4-10

7.- Viña, J., Servera, E., Asensi, M., Sastre, J., Pallardó, F.V., Gimeno, A., Heunks, L., Dekhuijzen, P.N.R., Ferrero, J.A. (2000) Exercise induces oxidative stress in healthy subjects and in chronic obstructive pulmonary disease patients  In: Handbook of Oxidants and Antioxidants in Exercise  Elsevier Science B.V. (Edited by: C.K. Sen, L. Packer and O. Hänninen)  Part XI, chapter 39, 1137 – 1146

8.- Viña J,   Gómez-Cabrera MC,  Lloret A,  Márquez R,  Miñana  JB, Pallardó  FV and Sastre  J (2000) Free Radicals in Exhaustive Physical Exercise: Mechanism of Production, and Protection by antioxidants. IUBMB Life. 271-277

9.- Desco MC, Asensi M, Marquez R, Martinez-Valls J, Vento M, Pallardo FV, Sastre J, Vina J. (2002) Xanthine oxidase is involved in free radical production in type 1 diabetes: protection by allopurinol. Diabetes 51(4):1118-24.

10.- Gómez-Cabrera  MC and Viña J (2003). Ejercicio Físico, entrenamiento y estrés oxidativo. Importancia de los nutrientes antioxidantes. Alimentación Nutrición y Salud. 10 : 71-81.

11.- Gómez-Cabrera MC, García-del-Moral L, Pallardó FV, Sastre J and Vina J (2003) Allopurinol and markers of muscle damage among participants in the Tour de France. JAMA 289 . Accepted for publication.

12.- Ji  LL,  Gómez-Cabrera MC,  Steinhafel N  and Viña  J (2004)  Acute Exercise Activates Nuclear Factor (NF) kB Signaling Pathway in Rat Skeletal Muscle. FASEB JOURNAL.  In Press.

 

 

 

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