Our research group is dedicated to a multi-scale study of evolutionary processes and the application of acquired knowledge to improve the health status of human collectives. This description is necessarily generic and ambiguous, and it focuses on a series of research activities that are detailed below:

• Epidemiology and evolution of pathogenic microorganisms. We take advantage of the research capability granting us access to genetic information (gene sequences and genomes) on recent history and evolutionary processes that act and have acted on microorganisms, normally bacteria and viruses, and enable follow-up and monitoring as a way to track the origin of transmission paths, the introduction and expansion of genes and drug-resistant variants, etc.
• Evolutionary systems biology. The recent developments in massive sequencing techniques and bioinformatics allow a rebuild of the evolutionary history of organisms, their genes and genomes, as well as that of components formed by all the various systems. The implementation of these methodologies in pathogenic organisms and their hosts makes us reach a better understanding on pathogenesis as well as alternatives and possibilities to act against them.
• Mutation and viral evolution (VIRMUT). A mutation represents the ultimate source for genetic variation and, as such, a key factor that clarifies the great variability and rapid evolution of ARN viruses. In this field, we estimated the virus mutation rate in animals, plants and bacteriophages (RNA as well as DNA ones). As of today, we are working on an in vitro and in vivo mutation rates estimate of different, biomedically relevant human viruses, such as HIV-1 or hepatitis C. With the use of different experimental approximations, we count on being able to detect mechanisms yet unknown in the creation of RNA diversity.
• Biologic complexity and robustness. The organisms’ capacity to withstand mutations (genetic or mutational robustness) determines the strength of natural selection and plays an important role in evolution. With the directed mutagenesis technique, we characterised the distribution of mutational effects based on the biological efficacy of various RNA viruses. This allowed us to observe notoriously low robustness levels. Moreover, our group pointed out the existence of a correlation between epistasis (interaction between genes or loci) and genomic complexity. The Systems Biology currently offers tools that allow to test these predictions.
• Experimental evolution of oncolytic viruses. Different RNA viruses show a certain degree of spontaneous selectiveness towards cancer cells, which is convenient in potential candidates for the development of therapeutic applications. The vesicular stomatitis virus (VSV) is a RNA virus with natural oncolytic activity and it’s usually used in our laboratory for studies on experimental evolution. The VSV adaptation to different cancer cell lines by experimental evolution will enable the obtainment of potential oncolytics, provided it results in a relevant decrease of its efficacy in primary cells. The virus candidates will be tested in vivo through infections in mice.