- Small molecule structure elucidation: elucidation of connectivity, stereochemical configuration and conformation.
- Structural characterisation of nanoparticles and their interactions with substrates.
- Mobility studies and molecular dynamics.
- Monitoring of chemical processes: kinetics.
- Determination of association and molecular interaction processes.
- Studies at variable temperatures.
- Observation of a wide variety of magnetic cores: 19F, 195Pt, 133Cs, 11B, 31P, 6Li, 29Si, etc.
Morphological, morphometric and molecular protozoa diagnosis; diagnosis of helminths and arthropod parasites of human and animal nature. Epidemiological modelling of human and animal parasitic diseases via creation of Geographical Information Systems (GIS).
Electrophoresis, in situ PCR, RT PCR, quantitative PCR, staining and detection methods, transfer analysis (western, southern...), recombinant DNA technology (DNA extraction, enzymatic treatment, DNA transfer ...), flow cytometry and "FACS" (Fluorescence-Activated Cell Sorter), genomic amplification (WGA, whole genome amplification), sample treatment for optical and electronic microscopy, inclusion, Immunohistochemistry, FISH.
Lab spaces disposition, equipped with the necessary inventory and fungible material to finalise investigations in detailed lines. Laboratories equipped with ELISA readers, mono- and two-dimensional electrophoresis systems, ChemiDoc XRS Bio-Rad analysis and documentation system, normal and real-time PCR thermocyclers (real-time PCR CFX96 Touch system; Bio-Rad). Electroporator, incubators, coolers, -20 and -80 freezers. Semi-wet transfer equipment. Refrigerated centrifuges with fixed and oscillating rotors, for various tube sizes. Floor and tabletop ultracentrifuges. Autoclaves, water purification systems. Optic and fluorescence microscopes. Staining trays and specific staining reagents. ELISA plate reader and real-time thermocycler.
Galaxy Badger, UGene, Artemis: Genome Browser, ACT, ClustalX, MrBayes, Mafft, RAxML, GS De Novo Assembler, GPRo, MIRA, Velvet, AphidBase, Staden, Unix Desktop Environment Project (UDE).
Analysis of genome and proteomics sequencing data, creation of phylogenetic trees, computer programming, BLASTO, study of biochemical networks
The bright-field microscope allows us to view stained or naturally pigmented samples that are highly contrasted. The source of illumination is white light. The components of the sample are visualised thanks to the differences in contrast between them and the surrounding medium.
The confocal microscope allows us to visualize images with different fluorescent labelling, therefore obtaining images of great sharpness and quality because the pictures earned are not contaminated by light emitted outside the focal plane. Thanks to this confocal characteristic, we can perform three-dimensional reconstructions from optical sections.
The variety of fluorescent proteins and multicolour probes that have been developed allows virtually any molecule to be tagged. The ability to visualise protein dynamics in vesicles, organelles, cells and tissues has provided new information about the functioning of cells in both healthy and diseased situations. This information includes the spatio-temporal dynamics of processes, such as mitosis; studies on the dynamics of ions, such as Ca+₂ and changes in the cytoskeleton.
Ability to address the DNA sequencing of genetic material from any organism using both traditional sequencing techniques (Sanger sequencing) as Next-Generation Sequencing (NGS).
3 laboratories, 9 offices, 12 jobs for predoctoral researchers, server room (6 jobs for researchers and 4 offices).
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
Fluorescence is one of the most widely used physical phenomena in biological and analytical microscopy, mainly because of its high degree of sensitivity and specificity. Fluorescence microscopy allows users to determine the distribution of a single molecule, its quantity and its location within a cell.
Fluorescence microscopes used in research applications are based on a series of optical filters. The filters are usually inserted in a filter block. While the excitation filter selects the wavelengths that excite a particular fluorophore within the sample, the emission filter acts as a kind of quality control, as it only allows the wavelengths of interest emitted by the fluorophore to pass through.
Refrigerated centrifuges, micropipettes, magnifiers, PCR Lifecycler, Orbital vacuum pumps, exhaust hoods and laminar flows, vortex, MiniSpin, cooker, electrophoresis buckets, Qubit 2.0, NanoDrop 1000, gas burner, photoperiod insect breeding chambers, scale, photo documentation equipment, thermal cyclers, freezers, refrigerators.
The Massarray platform is responsible for the research study of biological samples through the fine mapping of DNA sequences and the analysis of methylation phenomena. With this equipment, dozens of DNA variants such as SNPs, insertions or deletions can be analysed in hundreds of samples in a short time with a high degree of precision and sensitivity. It is also possible to analyse somatic mutations such as those present in samples of cancerous origin. Finally, the degree of methylation of individual CpGs in short DNA sequences can be quantitatively quantified.
Massive DNA sequencing equipment based on sequencing by synthesis, using nucleotides labelled with fluorophores of different colours, and capturing images of the emitted fluorescence.
NIKON DXM 1200C Digital Camera. NIKON E400 microscope. Progres CT3 USB Digital Camera; NIKON SMZ 745T Magnifier.
Molecular biology equipment: 2 thermal cyclers. 1 Gradient thermal cycler. Image Analyser for agarose gels. QIAxcel System for nucleic acid analysis. Laminar flow cabinet for bacteria culture and phage work. 2 colony counters.
Flow cytometry, qRT-PCR, Western Blot, Big-Data and primary cell culture techniques.
Equipment: 3 laminar flow cabinets for cell culture. 3 CO2 incubators. 1 Hypoxia workstation. Inverted microscope. Micromanipulator. -80ºC cabinet. Autoclave. Freezer.
Application of molecular phylogenetic inference methods to sequence data from ultra-sequencing to the determination of new genomes.
Equipment that performs determinations of materials by Raman Spectrophotometry coupled to a confocal microscope.
Real-time PCR is a technique that combines amplification and detection in a single step by correlating the PCR product of each cycle with a fluorescence intensity signal. They consist of a thermal cycler coupled to an optical system, which monitors the signal of the fluorophores used to detect the amplified product. Because the fluorescence of the fluorophores increases as the product is amplified, the amplification and detection processes are combined in a single step.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
The equipment has a cell separation system allowing the physical separation of a given population with different levels of purity. It allows separation under sterile conditions.
Laboratory with high-tech biomechanical tools and an important team of psychiatrists and psychologists making functional and neurocognitive assessments by offering three services:
- Neurocognitive Assessment: clinical and comprehensive neurocognitive assessments are carried out and the affected people as well their relatives are treated in order to guarantee a quality and independent life.
- Biomechanical Assessment of Human Functions: it provides objective and quantitative information on functional repercussions of a pathology in a person’s activities and functions, becoming an additional tool for health workers in the control of functional deficiencies of the musculoskeletal system.
- Gaze monitoring system (TOBII): it allows the recording and analysis of the gaze which can be used as an assessment and rehabilitation tool. Providing information in various fields of knowledge as an interpretation of human behaviour.
Chemi-Doc XRS gels documentation system (Bio-Rad). Floor and tabletop ultracentrifuges. Real-time PCR CFX96 Touch System (Bio-Rad). Fluorescence microscope. Image contrast microscope and digital video camera-imaging system. ELISA microplate readers. Mono and two-dimensional electrophoresis systems. Refrigerated centrifuges, freezers and coolers; orbital incubators; electroporator; sonicator; autoclaves and water purification systems.
Breeding and maintenance of insects, dissection of their central nervous system
This is an O2-CO2 gas analyser with air flow and two-animal switch. It contains: a software platform for metabolism, "respiratory metabolism" extension of the Metabolism software, single line Treadmill for mouse with shock, Calorimetry Runner for the treadmill, Rat/Mouse tray, Two Calorimetry lids for food and drink rats, Calorimetry Feeder/Drink, 100mL bottle for Oxylet system, Rat grid floor, Mouse grid floor. This equipment has been supervised by the specialist technician Javier Cuenca.