FUNGENAPHID

The project proposes the development of two types of tools to study gene functions in aphids. On one side, the project proposes an alternative RNAi methodology which consists in providing aphids with a continuous supply of the dsRNA required to trigger the RNAi by including it in a plant virus that infects the plant the aphid naturally feeds on. This technique, called VIGS (Virus Induced Gene Silencing), is a tool successfully used in the silencing of plant genes. Secondly, we intend to develop the CRISPR / Cas methodology in aphids. In addition to investigating the extension of these techniques to aphids, we will investigate the role of candidate genes that we have identified so far as good candidates to regulate several polyphenisms in aphids (including the reproductive polyphenism).

Acronym

FUNGENAPHID

Description

Title: Development of tools to study gene functions involved in the regulation of poliphenisms in aphids

Research Group: Evolutionary Genetics

The project proposes the development of two types of tools to study gene functions in aphids. On one side, the project proposes an alternative RNAi methodology which consists in providing aphids with a continuous supply of the dsRNA required to trigger the RNAi by including it in a plant virus that infects the plant the aphid naturally feeds on. This technique, called VIGS (Virus Induced Gene Silencing), is a tool successfully used in the silencing of plant genes. Secondly, we intend to develop the CRISPR / Cas methodology in aphids. In addition to investigating the extension of these techniques to aphids, we will investigate the role of candidate genes that we have identified so far as good candidates to regulate several polyphenisms in aphids (including the reproductive polyphenism).

Non-UV principal researchers

David Martínez Torres

Non-UV participating researchers

Paolo Cuti
Miquel Barberà Solà

Start date

2019 January

End date

2022 September

Funding agencies:

Ministerio de Ciencia, Innovación y Universidades