COV-CRISPIS

Traditional viral infection diagnostic techniques in the clinic are based on (RT-)PCR procedures, which are time consuming and require precise equipment and human resources, which preclude a rapid and massive intervention. Here, we will engineer a novel class of stand-alone biosystems aimed at diagnosing the presence of SARS-CoV-2. These biosystems consist of three reaction steps following sample collection without the need of sophisticated equipment: i) isothermal amplification of viral RNA, ii) CRISPR-based nucleic acid detection (working like an on-the-fly sequencing reaction), and iii) output disclosure by immunochromatographic assay.

Description

Title: Rapid, ultraspecific, and portable SARS-CoV-2 diagnostics based on CRISPR-Cas technologies and commercial immunochromatographic assay strips

Research groups: Biosystems Design and Non-Coding RNA-Mediated Regulatory Networks

Traditional viral infection diagnostic techniques in the clinic are based on (RT-)PCR procedures, which are time consuming and require precise equipment and human resources, which preclude a rapid and massive intervention. Here, we will engineer a novel class of stand-alone biosystems aimed at diagnosing the presence of SARS-CoV-2. These biosystems consist of three reaction steps following sample collection without the need of sophisticated equipment: i) isothermal amplification of viral RNA, ii) CRISPR-based nucleic acid detection (working like an on-the-fly sequencing reaction), and iii) output disclosure by immunochromatographic assay.

Non-UV principal researchers

Guillermo Rodrigo

Non-UV participating researchers

Gustavo Gómez (I2SYSBIO), José Antonio Daròs and Alberto Carbonell (IBMCP), and Rafael Ballesteros-Garrido, Pilar Campins-Falcó, Carmen Molins-Legua and Yolanda Moliner-Martínez (Chemistry Fac.)

Start date

2020 May

End date

2021 December

Funding agencies:

CRUE-Santander