I2SysBio, by means of the groups of its Programme of Biology of Systems of Pathogens, provides to academic and business researchers a wide number of technological tools for the manipulation and identification of human viruses and the development of techniques linked to applied virology in humans:

• High-throughput antiviral testing for human RNA viruses under BSL2 conditions, including mechanism of action studies and therapeutic window determination (toxicity versus efficacy). Available viruses include: respiratory syncytial virus, influenza virus A, Enteroviruses (EV-A71, EV-D68, Coxackievirus B3), rhinovirus B14, Seneca Valley virus, vesicular stomatitis virus, and sindbis virus. Additional screening capacity for entry inhibitors targeting MERS, Ebola, Nipah virus and Hendra virus.
• Antiviral testing for human RNA viruses under BSL3 conditions, including mechanism of action studies: SARS-CoV-2. Additional viruses can be tested as needed following protocol approval.
• Growth and purification of viruses. Amplification of known human viruses in cell culture and preparation of high titer stocks. Possibility for gradient purification.
• Metagenomics analysis of environmental or clinical samples for the detection of human viruses. Almost impartial enlargement of viral nucleoid acids in a sample provided by the user, sequencing the high performance and identification of viral sequences.
• Electronic microscopy of virus. Morphological analysis of viral particles in human tissues or the morphological analysis of purified viral preparations.
• Microscopy of automatized fluorescence in real time for the quantification of viral growth in cultivation. Infection of cell cultivation with virus marked with fluorescence provided by the user, quantification of the fluorescence in cultivations and analysis of the growth / viral expansion.
• Quantitative PCR in real time for the viral quantification of human samples. Extraction of DNA/ RNA, design of primers, qPCR and analysis of data.
• Research and characterisation of specific pathogen bacteria. Environmental samples and sample tests for detecting the presence of eaters that target the bacteria provided by the user (BSL-1 o BSL-2). Morphological (TEM) and genetic (NGS) characterisation of isolated eaters.
• Targeted evolution of viruses. Optimisation of a virus (infectiousness, growth rate, lithic capacity) by means of artificial selection in the laboratory. Characterisation of a developed virus (sequencing, infectiousness of the tests).
• Evaluation of efficacy in mice (pending ethics approval): respiratory syncytial virus and coxsackievirus B3.

Contact our innovation agent Carla Rubio (carla.rubio@csic.es) for more information.