2005
Conformation and concerted dynamics of the integrin binding site and the C-terminal region of echistatin revealed
by homonuclear NMR
Monleón, D., Esteve, V., Kovacs, H., Calvete, J.J. & Celda, B.
Biochem. J. 387 (2005) 57-66
Structure-function correlations of snake venom disintegrins
Calvete, J.J.
Curr. Pharm. Design 11 (2005) 829-835
Snake venom disintegrins: evolution of structure and function
Calvete, J.J., Marcinkiewicz, C., Monleón, D., Esteve, V., Celda, B., Juárez, P. & Sanz, L.
Toxicon 45 (2005) 1063-1074
Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes
vipera and Macrovipera lebetina
Bazaa, A., Marrakchi, N., El Ayeb, M., Sanz, L. & Calvete, J.J.
Proteomics 5 (2005) 4223-4235
cDNA cloning and functional expression of jerdostatin, a novel RTS-disintegrin from Trimeresurus jerdonii and a
specific inhibitor of the α1β1 integrin
Sanz, L., Chen, R-Q., Pérez, A., Hilario, R., Juárez, P., Marcinkiewicz, C., Monleón, D., Celda, B., Xiong, Y-L., Pérez-
Payá, E. & Calvete, J.J.
J.
Biol. Chem. 280 (2005) 40714-40722
2006
Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland
cDNA libraries. Insight into the evolution of the snake venom´s integrin inhibition system
Sanz, L., Bazaa, A., Marrakchi, N., Pérez, A., Chenik, M., Bel Lasfer, Z., El Ayeb, M. & Calvete, J.J.
Biochem. J. 395 (2006) 385-392
Molecular cloning of disintegrin-like transcript BA-5A from Bitis arietans venom gland cDNA library: a putative
intermediate in the evolution of the long chain disintegrin bitistatin
Juárez, P., Wagstaff, S.C., Oliver, J., Sanz, L., Harrison, R.A. & Calvete, J.J.
J. Mol. Evol. 63 (2006) 142-152
Molecular cloning of Echis ocellatus disintegrins reveals non-venom-secreted proteins and a pathway for the
evolution of ocellatusin
Juárez, P., Wagstaff, S.C., Sanz, L., Harrison, R.A. & Calvete, J.J.
J. Mol. Evol. 63 (2006) 183-193
Venom proteomes of closely-related Sistrurus rattlesnakes with divergent diets
Sanz, L., Gibbs, H.L., Mackessy, S.P. & Calvete, J.J.
J. Prot. Res. 5 (2006) 2098-2112
2007
KTS- and RTS-disintegrins: anti-angiogenic viper venom peptides specifically targeting the α1β1 integrin
Calvete, J.J., Marcinkiewicz, C. & Sanz, L.
Curr. Pharm. Des. 13 (2007) 2853-285
Loss of introns along the evolutionary diversification pathway of snake venom disintegrins bevidenced by sequence
analysis of genomic DNA from Macrovipera lebetina transmediterranea and Echis ocellatus
Bazaa, A., Juárez, P., Marrakchi, N., Bel Lasfer, Z., El Ayeb, M., Harrison, R.A., Calvete, J.J. & Sanz, L.
J. Mol. Evol. 64 (2007) 261-271
Snake venomics of Bitis gabonica gabonica. Protein family composition, subunit organization of venom toxins, and
characterization of dimeric disintegrins bitisgabonin-1 and bitisgabonin-2
Calvete, J.J., Marcinkiewicz, C. & Sanz, L.
J. Proteome Res. 6 (2007) 326-336
Proteomic mass profiling of a snake venom using high-mass detection MALDI-TOF mass spectrometry
Yanes, O., Wenzel, R., Calvete, J.J., Nazabal, A., Avilés, F.X., Zenobi, R.
J. Am. Soc. Mass Spectrom. 18 (2007) 600-609
Snake venomics. Protocols and applications
Calvete, J.J., Juárez, P. & Sanz, L.
J. Mass Spectrom. 42 (2007) 1405-1414
Snake venomics of Bitis species reveals large intragenus venom toxin composition variation. Application to
taxonomy of congeneric taxa
Calvete, J.J., Escolano, J. & Sanz, L.
J. Proteome Res. 6 (2007) 2732-2745
VEGF-related protein isolated from Vipera palestinae venom, promotes angiogenesis
Brown, M.C., Calvete, J.J., Staniszewska, I., Walsh, E.M., Perez-Liz, G., Del Valle, L.D., Lazarovici, P. & Marcinkiewicz,
C.
Growth Factors 25 (2007) 108-117
2008
Snake venomics of Central American pitvipers. Clues for rationalizing the distinct envenomation profiles of
Atropoides nummifer and Atropoides picadoi
Angulo, Y., Escolano, J., Lomonte, B., Gutiérrez, J.M., Sanz, L. & Calvete, J.J.
J. Proteome Res. 7 (2008) 708-719
Snake venomics of the South and Central American Bushmasters. Comparison of the toxin composition of Lachesis
muta gathered from proteomic versus transcriptomic analysis
Sanz, L., Escolano, J., Ferretti, M., Biscoglio, M.J., Rivera, E., Crescenti, E.J., Angulo, Y., Lomonte, B., Gutiérrez, J.M. &
Calvete, J.J.
J. Proteomics 71 (2008) 46-60
Snake venomics and antivenomics of the arboreal neotropical pitvipers Bothriechis lateris and Bothriechis schlegelii
Lomonte, B., Escolano, J., Fernández, J., Sanz, L., Angulo, Y., Gutiérrez, J.M. & Calvete, J.J.
J. Proteome Res.7 (2008) 2445-2457
Snake venomics of the lancehead pitviper Bothrops asper. Geographic, individual and ontogenetic variations
Alape-Girón, A., Sanz, L., Escolano, J., Flores-Díaz, M., Madrigal, M., Sasa, M. & Calvete, J.J.
J. Proteome Res. 7 (2008) 3556-3571
Snake venomics of the Armenian mountain vipers Macrovipera lebetina obtusa and Vipera raddei
Sanz, L., Ayvazyan, N. & Calvete J.J.
J. Proteomics 71 (2008) 198-209
Snake venomics of the Brazilian pitvipers Bothrops cotiara and Bothrops fonsecai. Identification of taxonomy
markers
Tashima, A.K., Sanz, L., Camargo, A.C.M., Serrano, S.M.T. & Calvete, J.J.
J. Proteomics 71 (2008) 473-485
Evolution of snake venom disintegrins by positive Darwinian selection
Juárez, P., Comas, I., González-Candelas, F. & Calvete, J.J.
Mol. Biol. Evol. 25 (2008) 2391-2407
Snake venomics of the Lesser Antillean pit vipers Bothrops caribbaeus and Bothrops lanceolatus. Correlation with
toxicological activities and immunoreactivity of a heterologous antivenom
Gutierrez, J., Sanz, L., Escolano, J., Fernandez, J., Lomonte, B., Angulo, Y., Rucavado, A., Warrell, D.A. & Calvete, J.J.
J. Proteome Res. 7 (2008) 4396-4408
2009
Snake population venomics: proteomics-based analyses of individual variation reveals gene regulation effects on
venom protein expression in Sistrurus rattlesnakes
Gibbs, H.L., Sanz, L. & Calvete, J.J.
J. Mol. Evol. 68 (2009) 113-125
Combined
snake venomics and venom gland transcriptomic analysis of the ocellated carpet viper, Echis ocellatus
Wagstaff, S.C., Sanz, L., Juárez, P., Harrison, R.A. & Calvete, J.J.
J. Proteomics 71 (2009) 609-623
Structural requirements of KTS-disintegrin for inhibition of α1β1 integrin
Brown, M.C., Eble, J.A., Calvete, J.J. & Marcinkiewicz, C.
Biochem. J. 417 (2009) 95-101
Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atroxasper
complex endemic to Venezuela: Contributing to its taxonomy and snakebite management
Calvete, J.J., Borges, A., Segura, A., Flores-Diaz, M., Alape-Girón, A., Gutiérrez, J.M., Diez, N., De Sousa, L., Kiriakos,
D., Sánchez, E., Faks, J.G., Escolano, J. & Sanz, L.
J. Proteomics 72 (2009) 227-240
Snake venomics and antivenomics: proteomic tools in the design and control of antivenoms for the treatment of
snakebite envenoming
Gutiérrez, J.M., Lomonte, B., León, G., Alape-Girón, A., Flores-Diaz, M., Sanz, L., Angulo, Y. & Calvete, J.J.
J. Proteomics 72 (2009) 165-182
Venoms, venomics, antivenomics
Calvete, J.J., Sanz, L., Angulo, Y., Lomonte, B. & Gutiérrez, J.M.
FEBS Lett 583 (2009) 1736-1743
Exploring the venom proteome of the western diamond rattlesnake, Crotalus atrox, via snake venomics and
combinatorial peptide ligand approaches
Calvete, J.J., Fasoli, E., Sanz, L., Boschetti, E. & Righetti, P.G.
J. Proteome Res. 8 (2009) 3055-3067
Studies on the venom proteome of Bothrops asper: perspectives and applications
Alape-Girón, A., Sanz, L., Escolano, J., Flores-Díaz, M., Madrigal, M., Sasa, M. & Calvete, J.J.
Toxicon 54 (2009) 938-948
Effect of VP12 and viperistatin on inhibition of collagen receptors-dependent melanoma metastasis
Staniszewska, I., Walsh, E.M., Rothman, V.L., Lazarovici, P., Calvete, J.J., Tuszynski, G.P., Gaathon, A. & Marcinkiewicz,
C.
Cancer Biol. Therapy 8 (2009) 1507-1516
Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú
and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards
paedomorphism
Núñez, V., Cid, P., Sanz, L., De La Torre, P., Angulo, Y., Lomonte, B., Gutiérrez, J.M. & Calvete, J.J.
J. Proteomics 73 (2009) 57-78
Snake venomics and disintegrins. Portrait and evolution of a family of snake venom integrin antagonists
Calvete, J.J., Juárez, P. & Sanz, L.
Handbook of Venoms and Toxins of Reptiles
Mackessy, S.P. (ed.)
CRC Press, Taylor & Francis, Boca Ratón
Chapter 17, pp. 337-357 (2009), ISBN: 978-0-8493-9165-1
Venomics
Calvete, J.J. (ed.)
J. Proteomics 72 (2), pp. 121-282, Elsevier, BV (2009)
doi:10.1016/S1874-3919(09)00082-7
2010
Preclinical assessment of the efficacy of a new antivenom (EchiTAb-Plus-ICP®) for the treatment of viper
envenoming in sub-Saharan Africa
Segura, A., Villalta, M., Herrera, M., León, G., Harrison, R., Durfa, N., Nasidi, A., Calvete, J.J., Theakston, R.D.G.,
Warrell, D.A. & Gutiérrez, J.M.
Toxicon 55 (2010) 369-374
Snake venomics of the Central American rattlesnake Crotalus simus and the South American Crotalus durissus
complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America
Calvete, J.J., Sanz, L., Cid, P., De La Torre, P., Flores-Díaz, M., Dos Santos, M.C., Borges, A., Bremo, A., Angulo, Y.,
Lomonte, B., Alape-Girón, A. & Gutiérrez, J.M.
J. Proteome Res.9 (2010) 528-544
Impact of regional variation in Bothrops asper snake venom on the design of antivenoms: integrating antivenomics
and neutralization approaches
Gutiérrez, J.M., Sanz, L., Flores-Díaz, M., Figueroa, L., Madrigal, M., Herrera, M., Villalta, M., León, G., Estrada, R.,
Borges, A., Alape-Girón, A. & Calvete, J.J.
J. Proteome Res. 9 (2010) 564-577
Antivenomics and venom phenotyping: a marriage of convenience to address the performance and range of clinical
use of antivenoms
Calvete, J.J.
Toxicon 56 (2010) 1284–1291
Exploring the venom proteome of the African puff adder, Bitis arietans, using a combinatorial peptide ligand library
approach at different pHs
Fasoli, E., Sanz, L., Wagstaff, S., Harrison, R.A., Righetti, P.G. & Calvete, J.J.
J. Proteomics 73 (2010) 932-942
Isolation of an acidic phospholipase A2 from the venom of the snake Bothrops asper of Costa Rica: biochemical and
toxicological characterization
Fernández, J., Gutiérrez, J.M, Angulo, Y., Sanz, L., Juárez, P., Calvete, J.J. & Lomonte,
B.
Biochimie 92 (2010) 273-283
Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP®, an Antivenom for
the Treatment of Snakebite Envenoming in sub-Saharan Africa
Calvete, J.J., Cid, P., Sanz, L., Segura, A., Villalta, M., León, G., Harrison, R., Durfa, N., Nasidi, A., Theakston, R.D.G.,
Warrell, D.A., Gutiérrez, J.M.
Am. J. Trop. Med. Hyg. 82 (2010) 1194-201
Snake venomics and antivenomics of Crotalus durissus subspecies from Brazil: assessment of geographic variation
and its implication on snakebite management
Boldrini-França, J., Corrêa-Netto, C., Silva, M.M.S., Rodrigues, R.S., De La Torre, P., Pérez, A., Soares, A.M., Zingali,
R.B., Nogueira, R.A., Rodrigues, V.M., Sanz, L. & Calvete, J.J.
J. Proteomics 73 (2010) 1758-1776
Isolation and biological characterization of Batx-I, a weak hemorrhagic and fibrinogenolytic PI metalloproteinase
from Colombian Bothrops atrox venom
Patiño, A.C., Pereañez, J.A., Núñez, V., Benjumea, D.M., Fernández, M., Rucavado, A., Sanz, L. & Calvete, J.J.
Toxicon 56 (2010) 936-943
Snake venomics of Bothriechis nigroviridis reveals extreme variability among palm pitviper venoms: different
evolutionary solutions for the same trophic purpose
Fernández, J., Lomonte, B., Sanz, L., Angulo, Y., Gutiérrez, J.M. & Calvete, J.J.
J. Proteome Res. 9 (2010) 4234-4241
Recombinant expression in human cells of active integrin α1β1-blocking RTS-disintegrin jerdostatin
Juárez, P., Bolás, G., de Rezende, F.F., Calvete, J.J. & Eble, J.A.
Toxicon 56 (2010) 1052-1058
Envenenamiento por mordedura de serpiente: La proteómica facilita el desarrollo de tratamientos para el ofidismo,
una patología desatendida
Calvete, J.J.
Investigación y Ciencia 405 (2010) 9-11
Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: relationship to the neutralization of
toxic and enzymatic activities
Antúnez, J., Fernández, J., Lomonte, B., Angulo, Y., Sanz, L., Pérez, A., Calvete, J.J. & Gutiérrez, J.M.
J. Venom Res. 1 (2010) 8-17
Snake venomics, antivenomics, and venom phenotyping: ménage à trois of proteomic tools aimed at understanding
the biodiversity of venoms
Calvete, J.J.
Toxins and Hemostasis: from Bench to Bedside (Kini, R.M., Markland, F., McLane, M.A., Morita, T., eds.)
Springer, Dordrecht (The Netherlands) Chapter 4, pp. 45-72 (2010), ISBN 9789048192946
Brief history and molecular determinants of snake venom disintegrin evolution
Calvete, J.J.
Toxins and Hemostasis: from Bench to Bedside (Kini, R.M., Clemetson, K., Markland, F., McLane, M.A., Morita, T., eds.)
Springer, Dordrecht (The Netherlands) Chapter 18, pp. 285-300 (2010), ISBN 9789048192946
2011
Snake venomics of African spitting cobras: Toxin composition and assessment of congeneric cross-reactivity of the
Pan-African EchiTAb-Plus-ICP® antivenom by antivenomics and neutralization approaches
Petras, D., Sanz, L., Segura, A., Herrera, M., Villalta, M., Solano, D., Vargas, M., León, G., Warrell, D.A., Theakston,
R.D.G., Harrison, R.A., Durfa, N., Nasidi, A., Gutiérrez, J.M. & Calvete, J.J.
J. Proteome Res. 10 (2011) 1266-1280
Snake population venomics and antivenomics of Bothrops atrox: paedomorphism along its transamazonian dispersal
and implications of geographic venom variability on snakebite management
Calvete, J.J., Sanz, L., Pérez, A., Borges, A., Vargas, A.M., Lomonte, B., Angulo, Y., Gutiérrez, J.M., Chalkidis, H.M.,
Mourão, R.H.V., Furtado, M.F.D. & Moura da Silva, A.M.
J. Proteomics 74 (2011) 510-527
Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae)
Fernández, J., Alape-Girón, A., Angulo, Y., Sanz, L., Gutiuérrez, J., Calvete, J.J. & Lomonte, B.
J. Proteome Res. 10 (2011) 1816–1827
Snake venomics and venom gland tranacriptomic analysis of Brazilian coral snakes, Micrurus altirostris and M.
corallinus
Corrêa-Netto, C., Junqueira de Azevedo, I.L.M., Silva, D.A., Ho, P.L., Leitão de Araujo, M., Alves, M.L.M., Sanz, L.,
Foguel, D., Zingali, R.B. & Calvete, J.J.
J. Proteomics 74 (2011)
Profiling venom gland transcriptomes of Costa Rican snakes by 454 pyrosequencing
Durban, J., Juárez, P., Angulo, Y., Lomonte, B., Flores-Diaz, M., Alape-Girón, A., Sasa, M., Sanz, L., Gutiérrez, J.M.,
Dopazo, J., Conesa, A. & Calvete, J.J.
BMC Genomics (2011)
NMR structure and dynamics of recombinant wild-type and mutated jerdostatin, a selective inhibitor of integrin
α1β1
Carbajo, R.J., Sanz, L., Mosulén S., Pérez, A., Marcinkiewicz, C., Pineda-Lucena, A. & Calvete, J.J.
PROTEINS: Structure, Function, and Bioinformatics (2011)
Ending the drought: new strategies for improving the flow of affordable, effective antivenoms in Asia and Africa
Williams, D.J., Gutiérrez, J.M., Calvete, J.J., Wüster, W., Ratanabanangkoon, K., Paiva, O., Brown, N.I., Casewell, N.R.,
Harrison, R.A., Rowley, P.D., O’Shea, M., Jensen, S.D., Winkel, K.D. & Warrell, D.
A.
J. Proteomics (2011)
Proteomic tools against the neglected pathology of snakebite envenoming
Calvete J.J.
Exp. Rev. Proteomics (2011)