Metatranscriptomic dynamics after Verticillium dahliae infection and root damage in Olea europaea
Paper published! ¡Artículo publicado! Metatranscriptomic dynamics after Verticillium dahliae infection and root damage in Olea europaea, Jan 2020, BMC Plant Biology, DOI 10.1186/s12870-019-2185-0.
Background: The olive tree is of particular economic interest in the Mediterranean basin. Researchers have conducted several studies on one of the most devastating disorders affecting this tree, the Verticillium wilt, which causes substantial economic losses in numerous areas. We analyzed metatranscriptomic samples taken from a previous study conducted on leaves and roots of Olea europaea that were infected with Verticillium dahliae (Jimenez-Ruiz, 2017). In addition, we also analyzed mechanically damaged roots. The aim of our approach is to describe the dynamics of the root microbiome after severe perturbations.
Results: Our results not only describe the dynamics of the microbial community associated with the disturbance, but also show the high complexity of these systems and explain how this can lead to a conflicting assignment of the various types of parasitism observed in a specific organism.
Conclusions: Our findings indicate that this infection, although led by Verticillium, is driven not by a single species, but by a polymicrobial consortium that also includes natural endophytes of the olive tree. This community contains both biotrophic and necrotrophic organisms that alternate and live together during the infection. In addition, opportunistic organisms appear that take profit not from plant tissues, but from new emerging populations of microorganisms. Therefore, this system can be described as a complex biological system composed of different interacting communities. Notably, our work has important considerations when it comes to classifying the type of parasitism of a given species.
Collaboration: This study is a collaboration between the University of Cordoba (Dept of Biochemistry and Molecular Biology), the LSC (Canfranc Underground Laboratory), and the I²SysBio (Theoretical and Computational Lab), with JMM currently working for Berkeley Lab (Biological Systems and Engineering Division) and LFAG now working for Institute for Sustainable Agriculture (CSIC).
Additional figures of the BMC PB paper (with full quality)
- Figure S1: Zero relative frequency (ZRF) histogram for all the taxa of samples during the process of infection with V. dahliae
- Figure S2: Taylor's law parameters summary plot for x-Weighted fit during the process of infection with V. dahliae for various Recentrifuge datasets
- Figure S3: Recentrifuge plots of fungal MTS classified reads at species level for leaves during V. dahliae infection
- Figure S4: Absolute frequency plot for reads of samples during the process of infection with V. dahliae
- Figure S5: Recentrifuge plot summarizing the results for suborder Tylenchomorpha 15 days after the inoculation with V. dahliae
- Figure S6: Clustered correlation and dendrogram plot for species during the process after Olea europaea root damage
Recentrifuge plots for Fungi
- Control Roots
- Roots infection after 48 hours
- Roots infection after 7 days
- Roots infection after 15 days
- Roots damage after 8 hours
- Roots damage after 24 hours
- Roots damage after 48 hours
- Roots damage after 7 days
- Control Leaves
- Leaves 15 days after Verticillium inoculation
- Leaves 15 days after root damage (Dikarya)
References
- Jimenez-Ruiz J, de la O Leyva-Perez M, Schiliro E, Bautista Barroso J, Bombarely A, Mueller L, Mercado-Blanco J, Luque F (2017). Transcriptomic Analysis of Olea europaea L. Roots during the Verticillium dahliae Early Infection Process. Plant Genome 10(1):1940-3372
- Martí JM (2019). Recentrifuge: robust comparative analysis and contamination removal for metagenomics. PLOS Computational Biology 15(4): e1006967. (Official website: www.recentrifuge.org)
Contact
For more information, please contact:
I²SysBio Theoretical and Computational Laboratory
Institute for Integrative Systems Biology
