Curriculum vitae
PERSONAL DATA
Name: Marcos
Family name: De la Peña Del Rivero
Address of work:
IBMCP (CSIC-UPV)
Av. de los Naranjos s/n
Valencia (Spain)
Tel: (34) 963877915
Fax: (34) 963877859
e-mail: rivero@ibmcp.upv,es
web page: http://www.uv.es/~pena
DEGREE
Graduate in Chemical Sciences (Biochemistry). Faculty of Chemistry, University of Valencia, 1990-1995.
M.D. in Chemical Sciences (Biochemistry), “Determination of the biological fitness in experimental populations of the MS2 bacteriophage”, University of Valencia, 1996.
Ph. D. in Biological Sciences, “Structure-function relationships in a viroid with hammerhead ribozymes”, University of Valencia, 2001.
RESEARCH ACTIVITY
- Research stage (6 months, 1993) in the GENEXPRESS group, GORLAEUS Laboratories, University of Leiden (The Netherlands): Report of stage "A study of the relationship between the MS2 5' domain and the A protein gene translation regulation" under the supervision of Drs. Herman Groeneveld and Jan van Duin.
- Research stage (1994-1997) in the Molecular Evolution group, Genetics Department, University of Valencia (Spain). Master thesis under the supervision of Dr. Andres Moya.
- Ph. D. thesis (1-4-1997 to 14- 12-2001) in the Department of Plant Stress, Institute for Molecular and Cellular Biology of Plants (UPV-CSIC, Spain) under the supervision of Dr. Ricardo Flores.
- Post-doctoral stage (1-7-2002 to 1-3-2005) in the RNA-protein Group at the European Molecular Biology Laboratories, Grenoble Outstation (France) under the supervision of Dr. Stephen Cusack.
- Research contract within the "Ramón y Cajal Program" (1-3-2005 to present) in the Department of Plant Stress, Institute for Molecular and Cellular Biology of Plants (UPV-CSIC, Spain).
FELLOWSHIPS AND CONTRACTS
ERASMUS fellowship for a 6 months stage in the Genexpress group, University of Leiden (The Netherlands).
ERASMUS-BANCAIXA fellowship for a 6 months stage in the Genexpress group, University of Leiden (The Netherlands).
Ph. D. fellowship from the MEC, Spain (1997-2001)
Specialization fellowship in international centers from the MCyT, Spain (2002)
Postdoctoral EMBO fellowship, EMBL Grenoble outstation, France (2003)
Postdoctoral HFSPO fellowship, EMBL Grenoble outstation, France (2003-2005)
Contract within the “Ramon y Cajal” program from the MCyT, Spain (2005-present)
MENTIONS AND OTHER AWARDS
Ph. D. thesis extraordinary award, University of Valencia (2002-2003).
PUBLICATIONS
1.- Groeneveld, H., De la Peña, M., Olsthoorn, R., van Duin, J.. “An in vivo recombination system to generate hybrid RNA viruses”. In Secondary structure of the bacteriophage MS2 RNA, Ph. D. Thesis of Dr. H. Groeneveld, chapter 6, University of Leiden, The Netherlands (1997).
2.- Flores, R., De la Peña, M., Navarro, J. A., Ambrós, S., Navarro, B. “Genetics of viroids”. In Molecular biology of viruses, chapter 11, pp. 225-239 (ed. C. L. Mandahar). Kluwer Academic Publishers, Norwell, MA, USA (1998).
3.- *De la Peña, M., *Navarro, B., Flores, R. “Mapping the molecular determinant of pathogenitiy in a hammerhead viroid: a tetraloop within the in vivo branched RNA conformation.” Proceedings of the National Academy of Sciences, USA, Vol. 96, pp. 9960-9965 (1999) (* authors contributed equally)
4.- Flores, R., Navarro, J.A., De la Peña, M., Navarro, B., Ambrós, S., Vera, A. “Viroids with hammerhead ribozymes: some unique structural and functional aspects with respect to other members of the group.” Biological Chemistry, USA, Vol. 96, pp. 9960-9965 (1999)
5.- De la Peña, M., Elena, S.F., Moya, A. “Effect of deleterious mutation-accumulation in the fitness of RNA bacteriophage MS2.” Evolution, Vol. 54, pp. 686-691 (2000)
6.- Flores, R., Hernández, C., De la Peña, M., Vera, A., Daròs, J.A. “Hammerhead ribozyme structure and function in plant RNA replication”. Methods in Enzymology (Eds-in-Chief: Abelson, J.N. and Simon, M.I.), Vol. 341, Ribonucleases, Part A (Ed: A. Nicholson), Academic Press, New York, NY, USA (2001)
7.- Flores, R., De la Peña, M., Navarro, B. “Chrysanthemum chlorotic mottle viroid”. In Viroid Diseases and their Control. (A. Hadidi, R. Flores, J.W. Randles, and J.S. Semancik, Eds). American Phytopathological Society Press, St. Paul, MN, USA (2001)
8.- Côté, F., De la Peña, M., Flores, R., Perreault, J.-P. “Ribozyme reactions of viroids.” In Viroid Diseases and their Control. (A. Hadidi, R. Flores, J.W. Randles, and J.S Semancik, Eds.). American Phytopathological Society Press, St. Paul, MN, USA (2001)
9.- Elena, S.F., Dopazo, J., De la Peña, M., Flores, R., Diener, T.O., Moya, A. “Phylogenetic analysis of viroids and viroid-like satellite RNAs from plants: a reassessment.” Journal of Molecular Evolution, Vol. 53, pp. 155-159 (2001)
10.- De la Peña, M., Flores, R. “An extra nucleotide in the consensus catalytic core of a viroid hammerhead ribozyme: Implications for a more efficient ribozyme design”. Journal of Biological Chemistry, Vol. 276, pp. 34586-34593 (2001)
11.- van Meerten, D., Groeneveld, H., Miller, D.M., Marechal, G.B., Tsareva, N.V., Olsthoorn, R.C., De la Peña, M., van Duin, J. “In vivo generation of hybrids between different species of RNA phages”. Journal of General Virology, Vol. 83, pp. 1223-1235 (2002)
12.- De la Peña, M., Flores, R. "Chrysanthemum Chlorotic Mottle Viroid RNA: Dissection of the Pathogenicity determinant and Comparative Fitness of Symptomatic and Non-symptomatic Variants". Journal of Molecular Biology, Vol. 321, pp. 411-421 (2002)
13.- De la Peña, M., Gago, S., Flores, R. "Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity". EMBO Journal, Vol. 22, pp. 5561-5570 (2003)
14.- De la Peña, M. "Structure-function relationships in a viroid with hammerhead ribozymes". Ph. D. thesis, University of Valencia (2003)
15.- Flores, R., Delgado, S., Gas, M.E., Carbonell, A., Molina, D., Gago, S., De la Peña, M. "Viroids: the minimal non-coding RNAs with autonomous replication". FEBS Letters, Vol. 567, pp. 42-48 (2004)
16.- *Gago, S., *De la Peña, M., Flores, R. "A kissing-loop interaction in a hammerhead viroid RNA critical for its in vitro folding and in vivo viability". RNA, Vol. 11 pp. 1073-1083 (2005)
17.- Carbonell, A., De la Peña, M., Flores, R., Gago S. "Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: differences in co- and post-transcriptional self-cleavage may explain the lack of trinucleotide AUC in most natural hammerheads". Nucleic Acids Research, Vol. 34, pp. 5613-5622 (2006)
18.- Flores, R., Navarro, B., Gago, S., De la Peña, M. "Chrysanthemum Chlorotic Mottle Viroid: a System for Reverse Genetics in the Family Avsunviroidae". Plant Viruses, Vol. 1(1), pp. 27-32 (2007)
19.- De la Peña, M., Kyrieleis, O., Cusack S. "Structural insights into the mechanism and evolution of the cap methyl-transferase sub-complex of the vaccinia virus". EMBO Journal, Vol. 26(23), pp 4913-4925 (2007)
20.- Flores, R., Carbonell, A., De la Peña, M., Gago S. "RNAs autocatalíticos: ribozimas de cabeza de martillo". Herramientas Biotecnológicas en Fitopatología (Eds. C. Fenoll, J. Marcos, V. Pallás y P. Rodríguez-Palenzuela) ISBN: 978-84-8476-319-2 (2008)
21.- *Dufour D, *de la Peña M, Gago S, Flores R, Gallego J. "Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads". Nucleic Acids Research, Vol. 37(2), pp. 368-381 (2008)
22.- Wulff, B.B.H., Heese, A., Tomlinson-Buhot, L., Jones, D.A., De la Peña, M., Jones, J.D.G. “The major specificity-determining amino acids of the tomato Cf-9 disease resistance protein are at hypervariable solvent-exposed positions in the central leucine-rich repeats” Molecular Plant-Microbe Interactions, Vol. 22(10), pp. 1203-1213 (2009) (This work was selected for the cover of the MPMI Journal)
23.- Verhoeven, J.Th.J., Jansen, C.C.C., Roenhorst, J.W., Flores, R., De la Peña, M. “Pepper chat fruit viroid: biological and molecular properties of a proposed new species of the genus Pospiviroid.” Virus Research, Vol. 144(1-2), pp. 209–214 (2009)
24.- Flores, R., Gas, M.E., Molina-Serrano, D., Nohales, M.A., Carbonell, A., Gago, S., De la Peña, M., Daròs, J.A. “Viroid Replication: Rolling-Circles, Enzymes and Ribozymes” Viruses, Vol. 1(2), pp. 317-334 (2009)
25.- De la Peña M, Dufour D, Gallego J. "Three-way RNA junctions with remote tertiary contacts: A recurrent and highly versatile fold". RNA, Vol. 15(11), pp. 1943-1958 (2009)
26.- De la Peña M, García-Robles I. "Intronic hammerhead ribozymes are ultraconserved in the human genome". EMBO Rep, 11(9):711-6 (2010)
27.- De la Peña M, García-Robles I. "Ubiquitous presence of the hammerhead ribozyme motif along the tree of life". RNA, 16(10):1943-1950 (2010)
28.- Kalweit A, Przybilski R, Seehafer C, de la Peña M, Hammann C. "Characterization of hammerhead ribozyme reactions". Methods Mol Biol. 848:5-20 (2012)
29.- Hammann C, Luptak A, Perreault J, de la Peña M. "The ubiquitous hammerhead ribozyme". RNA, 18(5):871-885 (2012)
30.- García-Robles I, Sánchez-Navarro J, de la Peña M. "Intronic hammerhead ribozymes in mRNA biogenesis". Biological Chemistry, in press (2012)
(Publications by Marcos De la Peña listed in the National Library of Medicine)
MEETINGS
Flores, R., Ambrós, S., Hernández, C., Navarro, J.A., De la Peña, M., Navarro, B. Propiedades de la familia de viroides con ribozimas de cabeza de martillo. XXI Congreso de la SEBBM, Sevilla, 1998.
De la Peña, M., Navarro, B., Flores, R. Identificación de determinantes de patogenicidad en el viroide del moteado clorótico del crisantemo. IX Congreso de la SEF, Salamanca, 1998.
De la Peña, M., Navarro, B., Flores, R. The pathogenicity determinant of chrysanthemum chlorotic mottle viroid maps at a tetraloop. XI International Congress of Virology, Sidney, 1999.
De la Peña, M., Flores, R. Análisis por mutagénesis dirigida del determinante de patogenicidad del viroide del moteado clorótico del crisantemo. X Congreso de la SEF, Valencia, 2000.
De la Peña, M., Flores, R. Un nucleótido extra en la ribozima de cabeza de martillo. XXIV Congreso de la SEBBM, Valencia, 2001.
Carbonell, A., De la Peña, M., Flores, R. Degradación in vitro de un viroide nucleolar mediada por una ribozima de cabeza de martillo incluida en el snoRNA U3 que actúa como vector de transporte al nucleolo. VII Congreso de la SEV, Valencia, 2001.
De la Peña, M., Flores, R. Chrysanthemum chlorotic mottle viroid: Dissection of the pathogenicity determinant, comparative fitness of symptomatic and non-symptomatic variants, and characterization of a kissing-loop interaction in the in vivo RNA conformation. XIV International Congress of Virology, Paris, 2002.
Gago, S., De la Peña, M., Flores, R. Un pseudonudo en la estructura de un RNA viroidal es crítico para su infectividad: evidencias in vivo. VII Congreso Nacional de Virologia, Barcelona, Spain, 2003.
De la Peña, M., Seraphin, B. and Cusack, S. Structural basis for 5' mRNA modifying enzymes: capping and decapping factors in human cells. IV HFSP Awardees Meeting, Hakone, Japan, 2004.
Flores R., De la Peña, M., Gago, S., Delgado, S., Gas, ME, Carbonell, A., Martínez de Alba, E., Hernández, C., Daròs, J.A. Viroid replication. Second European Congress of Virology Euro Virology 2004, Madrid, España, 2004
De la Peña, M., Cusack, S. Structural basis of the mRNA capping machinery fom Vaccinia virus, V HFSP Awardees Meeting, Bethesda, MD, USA, 2005
Gago, S., De la Peña, M., Flores, R. A kissing-loop interaction in Chrysanthemum chlorotic motle viroid critical for its in vitro folding and in vivo viability, XIII International Congress of Virology, San Francisco, CA USA, 2005
De la Peña, M., Cusack, S., Crystal structure of the mRNA cap 7-N-methyltransferase from vaccinia virus, VI HFSP Awardees Meeting, Paris, France, 2006
De la Peña, M., Cusack, S. Crystal structure of the Guanine N7-methyltransferase sub-complex from vaccinia virus mRNA capping enzyme. XIth RNA society meeting, Seattle, WA, USA, 2006
Dufour D, de la Peña M, Gago S, Flores R, Gallego J. Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads. XIIIth RNA society meeting, Berlin, Germany, 2008
Curriculum vitae (PDF)( (English))
Curriculum vitae (PDF) (Spanish)