The same method as in (2) was used, giving 3f_s / T_s = 1.4 ´ 10^-5 and c = 7 (48 h post inoculation with a generation time of 7 h, as estimated in one-step growth curves; note: the estimated bust size is B = 50 as shown in Figure 2 of the original publication) (1). Hence, min[m_s/n/c] = 2.0 ´ 10^-6. The estimated selection correction factor using the exponential plus lethal class model with E(s_v) = 0.12, p_L = 0.3, c = 7, selective sampling, and B = 50 is a = 0.28, thus giving m_s/n/c = 2.0 ´ 10^-6 / 0.28 = 7.1 ´ 10^-6.

1. Nobusawa, E. and K. Sato. 2006. Comparison of the mutation rates of human influenza A and B viruses. J Virol. 80:3675-3678.

2. Parvin, J. D., A. Moscona, W. T. Pan, J. M. Leider, and P. Palese. 1986. Measurement of the mutation rates of animal viruses: influenza A virus and poliovirus type 1. J Virol. 59:377-383.