The same method as in (2) was used, giving 3fs / Ts = 1.4 ´ 10-5 and c
= 7 (48 h post inoculation with a generation time of 7 h, as estimated in
one-step growth curves; note: the estimated bust size is B = 50 as shown in Figure 2 of the original publication) (1). Hence, min[ms/n/c] = 2.0 ´ 10-6. The
estimated selection correction factor using the exponential plus lethal class
model with E(sv) = 0.12, pL = 0.3, c = 7, selective sampling, and B = 50 is a = 0.28, thus giving ms/n/c =
2.0 ´ 10-6 / 0.28 = 7.1 ´ 10-6.
1. Nobusawa, E. and K. Sato. 2006. Comparison of
the mutation rates of human influenza A and B viruses. J Virol. 80:3675-3678.
2. Parvin, J. D., A. Moscona,
W. T. Pan, J. M. Leider, and P. Palese.
1986. Measurement of the mutation rates of animal viruses: influenza A virus and poliovirus type 1. J Virol. 59:377-383.