The average monoclonal antibody resistance frequency obtained from many small cultures undergoing one cell infection cycle or less was determined (1), giving f / c = 3.5 ´ 10^-5. It was assumed that T = T_s = 6 from references therein. These substitutions were probably close to neutral (a » 1), although this was not directly shown. Under this assumption, m_s/n/c = 3 ´ 3.5 ´ 10^-5 / 6 = 1.8 ´ 10^-5. The data from this experiment can also be used to estimate the mutation rate per strand copying using the fluctuation test null-class method.

1. Furió, V., A. Moya, and R. Sanjuan. 2005. The cost of replication fidelity in an RNA virus. Proc. Natl. Acad. Sci. USA 102:10233-10237.