Type: Equipment
A three-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
Flow cytometer analyser with 3 lasers (405nm, 488nm and 640nm).
Detection of 10 parameters (8 fluorescence and 2 light scattering).
HTS system for automated sample acquisition with capacity for 40 tubes.
Uses FACSSuite software for acquisition and analysis.
|
FILTER CONFIGURATION FACSVERSE |
||
|
DETECTOR BLUE 488nm |
MIRROR |
FILTER |
|
A |
752 LP |
783/56 |
|
B |
665 LP |
700/54 |
|
C |
---- |
---- |
|
D |
560 LP |
586/42 |
|
E |
507 LP |
527/32 |
|
F |
10 |
488/15 |
|
DETECTOR Red 640nm |
MIRROR |
FILTER |
|
A |
752 LP |
783/56 |
|
B |
660 /10 |
660/10 |
|
DETECTOR Violet 405nm |
MIRROR |
FILTER |
|
A |
500 LP |
528/45 |
|
B |
448/45 |
448/45 |
In addition, the laboratory has the following auxiliary equipment:
|
EQUIPMENT |
BRAND AND MODEL |
NUMBER |
INVENTORY CODE |
DESCRIPTION |
|
CABINA DE SEGURIDAD BIOLÓGICA |
TELSTAR BIO-II ADVANCE 3 |
1 |
72-210580 |
Type IIA biosafety cabinet for sample preparation. |
|
CENTRIFUGE
|
EPPENDORF 5702 |
1 |
72-217839 |
Centrifuge for 5, 15 and 50 mL tubes with a maximum speed of 5,500 rpm. |
|
THERMBLOCK |
FISHERBRAND |
1 |
72-214958 |
Digital dry bath for 1.5ml tubes |
|
REFRIGERATOR AND FRIDGE LAB |
SANYO MPR 414F |
1 |
72-163852 |
COMBI with 340L refrigerator and 82L freezer for reagent storage. |
|
TUBE SHAKER |
HEIDOLPH Reax-Top |
1 |
72-143423 |
Vortex for sample and reagent agitation. |
|
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Detection of cell subpopulations (immunophenotyping)
Apoptosis, viability, and cell cycle assays.
Functional assays (metabolism, oxidation, proliferation, intracellular calcium, pH, etc.)
Subcellular level studies (mitochondria, etc.)
Studies with prokaryotic cells, yeasts and algae
Absolute quantifications
Multiplexed assays (beads, arrays)
Advice to potential users on the applicability of this technique to their research.
ISO 9001:2015 certification
Annual review of the cytometer by an external company
To be an authorised user of the Flow Cytometry Service. To this end, you must inform about the project, the collaborators who will be working on it, the use of biological agents and be familiar with the working rules. To become an independent user, you must receive adequate training from the technicians of the section.
- Flores Raga, Ana
- PAS-E.T.S. Investigacio
- Norte Pinto, Sandra Cristina
- PIT-Investigacio Escala Tecnica Mitjana
