Room located inside the Cell Culture laboratory (BCL2) composed of a closed Hypoxia Station and necessary equipment for the maintenance of the cultures. The closed station allows a precise control of the percentage of hypoxia and/or hyperoxia, even at extreme values, keeping the culture conditions constant at all times, both during its handling and within the incubators. All of this ensures reproducible data.

Containment level 2 room with HEPA filtration, PVC scotch walls and own access control. Equipped with a modular and closed BIOSPHERIX X VIVO Hypoxia Station, with a working chamber where samples are handled, 4 internal incubators with independent sensors, an auxiliary chamber with equipment and a gate module with decontamination cycles for the entry and exit of material.

It allows to set a different oxygen level for each incubator and for the working chamber (between 0 and 90%), as well as a constant value of CO₂ and temperature. In this way, it is possible to have 4 different O₂ and CO₂ conditions and adapt the working chamber to the required incubator condition. The cultures remain at all times in a controlled and constant environment by avoiding contact with the outside atmosphere that occurs when opening and closing the doors in conventional incubators.

The X VIVO software, installed on an external computer, allows the conditions of the station to be easily changed and monitored.

The hypoxia station is simultaneously connected to a nitrogen generator and an outdoor distribution plant with N₂, O₂ and CO₂ cylinders to guarantee a continuous supply of gases.

Inside the station we have an inverted microscope, a tube centrifuge (15 and 30 mL), a vacuum pump and a complete set of micropipettes.

The room also has O₂ and CO₂ detectors installed to ensure user safety.

Integrated resources in the hypoxia room: