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Preparation, assembly and analysis of microbial genomes

Complete genome sequencing of a microbial strain
Preparation, assembly and analysis of microbial genomes
Type: Techniques

DNA extraction, whole genome sequencing (WGS), assembling, determination of quality parameters, genomic analysis...

Description

The complete genome sequencing is a technique increasingly used in microbial taxonomy. The gnomic sequence of a strain (complete or draft) makes it possible to study and characterise the microorganism by means of genomic analysis (annotation of genes, detection of genomic islands, etc.). But it also allows phenotypic inference studies to establish correspondences between the genomic sequences observed and the phenotypic characteristics of strain.

In addition, genomic sequencing can be used to obtain genome characteristics, such as size in base pairs, G+C content, total protein content, RNAs, presence of CRISPR, etc., as well as genomic data useful in taxonomy, such as Average Nucleotide Identity (ANI), Average Amino Acid Identity (AAI) or estimated in silico DNA-DNA Hybridisation (eDDH).

Practical implementation
  • Identification and taxonomic studies.
  • Microbial characterisation (bioprospecting, food safety assessment, etc.).
Quality certifications

ISO 9001:2015

Terms of use

The user must send an active culture of each strain (triple streak seeded Petri dish in the case of prokaryotes and yeasts; slant agar tube is also accepted in the case of filamentous fungi).

Contact: tlucena@cect.org, bacterias@cect.org and hongos@cect.org