Dr Agustín Aranda
Biochemistry and Molecular Biology department,
University
of Valencia
and Biotechnology Department, IATA
(CSIC)
Apartado 73, 46100 Burjassot (Valencia) SPAIN
Research interest
Stress response in the
yeast Saccharomyces cerevisiae during the processes involved in wine
making. We are characterizing the stress response induced by acetaldehyde in
laboratory strains and in industrial flor strains (involved in the
biological aging of Sherry wines), the role of the Btn2p protein in stress
response and biofilm formation and the stress caused by sulfur dioxide and its
relationship to adenine metabolism. Our aim is to link oxidative stress to
chronological ageing and apoptosis of wine yeast during grape must
fermentation. We are also studying the transcriptional regulation of SPI1,
a gene expressed during stationary phase and highly transcribed at the end of
vinification.
Recent publications
Aranda,
A. y Proudfoot N.J. (1999). Definition of transcriptional pause
elements in fission yeast. Mol. Cell. Biol. 19,
1595-1604.
Greger, I.H., Aranda, A. y Proudfoot N.J. (2000). Balancing transcriptional interference and initiation on the GAL7 promoter of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 97, 8415-8420.
Aranda, A. y Proudfoot N.J. (2001). Transcriptional termination factors
for RNA polymerase II in yeast. Mol. Cell 7, 1003- 1011.
Aranda,
A.; Querol, A. and y Olmo, M. (2002) Correlation between acetaldehyde and
etanol resístanse and expresión of HSP genes in yeast strains isolated during
biological aging of sherry wines. Arch. Microbiol. 177,
304-312.
Alén,
A., Kent, N.A., Jones, H.S., O’Sullivan, J., Aranda, A., y
Proudfoot, N.J. (2002). A role for chromatin remodelling in
transcriptional termination by RNA polymerase II. Mol. Cell 10,
1441-1452..
Aranda, A. y del Olmo, M. (2003) Response to acetaldehyde stress in the yeast Saccharomyces cerevisiae involves a strain dependent regulation of several ALD genes and is mediated by the general stress response pathway. Yeast 20, 747-759.
Aranda A, del Olmo ML. (2004) Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively. Appl Environ Microbiol. 70(4):1913- 22.
Garcia-Martinez J, Aranda A, Perez-Ortin
JE. (2004) Genomic run-on evaluates
transcription rates for all yeast genes and identifies gene regulatory
mechanisms. Mol Cell. 15(2):303-13.
Agustín
Aranda, Elena Jiménez-Martí, Helena Orozco, Emilia Matallana, Marcel.lí del
Olmo (2006) Sulfur and adenine metabolisms are
linked and both modulate sulfite resistance in wine yeast. J. Agric. Food
Chem. 54: 5839-5846.
Cardona,
F., Carrasco, P., Pérez-Ortín, J.E., del Olmo*, M. y Aranda, A (2007) A
novel approach for the improvement of stress resistance in wine yeast. Int J Food Microbiol. 114(1):83-91.
Espinazo-Romeu M, Cantoral
JM, Matallana E, Aranda A. (2008) Btn2p
is involved in ethanol tolerance and biofilm formation in flor yeast. FEMS Yeast Res. 2008 Jun 12. [Epub ahead of print]
Supplementary data to
Exposure to acetaldehyde in yeast determines an induction of
sulfur amino acid metabolism and polyamine transporter genes,
which depends on Met4p and Haa1p transcription factors respectively
Agustín Aranda and Marcel.lí del Olmo, Applied and Environmental
Microbiology, 70(4):1913- 22.
Microarray data
Microarrays experiments
showing changes in transcription in W303 strain exposed to 1g/l acetaldehyde
for one hour.
Control vs Acetaldehyde (raw data)
Acetaldehyde vs Control (raw data)
Table I. Transcript levels
of genes repressed by acetaldehyde. Average of the data obtained
from fluor reversed labeling of control and acetaldehyde treated cells
|
ORF |
Gene |
Log2
value |
Repression
Ratio |
|
YOR359W |
PDS1 HCM1 ACO1 ASH1 BCS1 OAC1 FAR1 DCC1 FKH1 GIC1 CDC14 HAP4 HOS3 CLN2 TOF2 RML2 EFB1 |
-3,6976155
|
12,97457648
|
Table II. Transcript levels
of genes induced by acetaldehyde. Average of the data obtained from
fluor reversed labeling of control and acetaldehyde treated cells.
|
ORF |
Gene |
Log2
value |
Induction
Ratio |
|
YCR021C |
SPI1 OYE3 ICY2 LEE1 THI4 HXK1 HSP104 PHD1 ASI3 APG5 MGA1 MUP1 GUP2 MET3 DAK2 GTT2 AAD14 THI13 TPO4 AAD15 FES1 AYT1 MHT1 APJ1 IKS1 OSW2 PIR3 BNA3 NRG1 FLO9 FLO5 GLC3 SET6 STF2 STI1 SFK1 MXR1 GRE3 SNA4 PXA1 MOH1 HXT11 MET10 PRB1 CYS3 XBP1 PDR12 TOS3 RTN2 BIO5 GPH1 GGA1 HCA4 MPH3 NOP14 YPS5 |
8,149884459 |
284,0270371 |
