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ProteomicsMain Researcher
Manuel Sánchez del PinoHead of the Proteomics Core Facility of the Centro de Investigación Príncipe Felipe (CIPF). Sánchez del Pino started as a graduate student at the Department of Biochemistry and Molecular Biology at the University of Valencia where he got his Bsc working in Histone acetylation. In 1990 he moved to the Department of Physiology and Biophysics at the Finch University of Health Scicences/The Chicago Medical School in North Chicago (Illinois, EEUU, now Rosalind Franklin University). He completed his PhD working on Neutral Amino Acid Transport Across the Blood-Brain-Barrier using Membrane Vesicles in the laboratory of Dr. Richard A. Hawkins. After one more year working in the same Department he moved, in 1995, to Cambridge University (UK) to on Protein Folding in the laboratory of Sir Alan R. Fersht as a postdoctoral fellow. Sánchez del Pino came back to Spain at the end of 1998 to join the laboratory of Dr. Jesé Mª Mato at the University of Navarra. There he worked in the Regulatory Mechanisms of Liver Methionine Adenosyl Transferase as well as in the creation of the University’s Proteomics Unit. At the end of 2003 he moved to the Fundación Valenciana de Investigaciones Biomédicas (former Instituto Citológico and current Centro de Investigación Príncipe Felipe) to set up there the new Proteomics Core Facility where he is still working. Research interest
Members of research group
Luz Valero, PhD. Lab Manager Technician
Laura Cantero, PhD. ProteoRed Lab Technician Esther Dionís. Lab Technician Virginia Rejas. Lab Technician Grants as PI since 2006Characterization of the mechanism of action of new apoptosis and cell cycle inhibitors with therapeutic potential using a proteomics approach. Instituto de Salud Carlos III (PI061718). Early biomarker discovery for bladder cancer using proteomics techniques. Generalitat Valenciana. Conselleria de Sanitat (AP081/07) Selected publicationsMora, L., Valero, M. L., Sánchez del Pino, M. M., Sentandreu, M. A. & Toldra, F. (2011). Small peptides released from muscle glycolytic enzymes during dry-cured ham processing. J Proteomics 74, 442-450. García-Murria, M. J., Valero, M. L. & Sánchez del Pino, M. M. (2011). Simple chemical tools to expand the range of proteomics applications. J Proteomics 74, 137-50. Escobedo-Lucea, C., Ayuso-Sacido, A., Xiong, C., Prado-López, S., Sánchez del Pino, M. M., Melguizo, D., Bellver-Estellés, C., González–Granero, S., Valero, L., Moreno, R., Burks, D. J. & Stojkovic, M. (2011). Development of a Human Extracellular Matrix for Applications Related with Stem Cells and Tissue Engineering. Stem Cell Reviews and Reports (DOI 10.1007/s12015-011-9270-6). Tenorio-Laranga, J., Valero, M. L., Mannisto, P. T., Sánchez del Pino, M. M. & Garcia-Horsman, J. A. (2009). Combination of snap freezing, differential pH two-dimensional reverse-phase high-performance liquid chromatography, and iTRAQ technology for the peptidomic analysis of the effect of prolyl oligopeptidase inhibition in the rat brain. Anal Biochem 393, 80-87. Laserna, E. J., Valero, M. L., Sanz, L., Sánchez del Pino, M. M., Calvete, J. J. & Barettino, D. (2009). Proteomic Analysis of Phosphorylated Nuclear Proteins Underscores Novel Roles for Rapid Actions of Retinoic Acid in the Regulation of mRNA Splicing and Translation. Mol Endocrinol 23, 1799-1814. Bech-Serra, J. J., Borthwick, A., Colome, N., ProteoRed, C., Albar, J. P., Wells, M., Sánchez del Pino, M. M. & Canals, F. (2009). A multi-laboratory study assessing reproducibility of a 2D-DIGE differential proteomic experiment. J Biomol Tech 20, 293-6. Mora, P., Carbajo, R. J., Pineda-Lucena, A., Sánchez del Pino, M. M. & Pérez-Payá, E. (2008). Solvent-exposed residues located in the b-sheet modulate the stability of the tetramerization domain of p53. A structural and combinatorial approach. Proteins 71, 1670-85. Sánchez del Pino, M. M., Pérez-Mato, I., Sanz, J. M., Mato, J. M. & Corrales, F. J. (2002). Folding of dimeric methionine adenosyltransferase III: identification of two folding intermediates. J Biol Chem 277, 12061-6. Sánchez del Pino, M. M., Corrales, F. J. & Mato, J. M. (2000). Hysteretic behavior of methionine adenosyltransferase III. Methionine switches between two conformations of the enzyme with different specific activity. J Biol Chem 275, 23476-82. Mateu, M. G., Sánchez del Pino, M. M. & Fersht, A. R. (1999). Mechanism of folding and assembly of a small tetrameric protein domain from tumor suppressor p53. Nat Struct Biol 6, 191-8. Sánchez del Pino, M. M. & Fersht, A. R. (1997). Nonsequential unfolding of the alpha/beta barrel protein indole-3-glycerol-phosphate synthase. Biochemistry 36, 5560-5. Sánchez del Pino, M. M., López-Rodas, G., Sendra, R. & Tordera, V. (1994). Properties of the yeast nuclear histone deacetylase. Biochem J 303 ( Pt 3), 723-9. Sánchez del Pino, M. M., Hawkins, R. A. & Peterson, D. R. (1992). Neutral amino acid transport by the blood-brain barrier. Membrane vesicle studies. J Biol Chem 267, 25951-7. Contact Information
Centro de Investigación Príncipe Felipe Teléfono. 34-963289680; XT 2102 E-mail: mspino@cipf.es |
