Institute for Integrative Systems Biology (I2SysBio)





Viral mutation rates


University of Valencia

Genetics Department



Avida image



Virus experimental evolution (Sanjuán lab)

Research tools

Experimental evolution
We use evolution in the lab to test evolutionary hypotheses and to direct traits of interest towards predefined goals for applied purposes (directed evolution). To achieve these goals, we have used bacteriophages, vesicular stomatitis virus (VSV), HIV-1, hepatitis C virus, enteroviruses, viroids, and adenoviruses, among other viruses.

Molecular and cell biology
The techniques we are currently using include massive parallel sequencing (including high-fidelity sequencing techniques), genetic engineering and gene synthesis, site-directed mutagenesis, RT-qPCR, single-cell analysis, automated quantitative microscopy, electron microscopy, etc.

Comparative biology
Comparing species is a classical approach in biology. We combine this approach with experimentation. One of our main research goals is to compare the evolutionary properties of different viral species, including their ability to generate genetic variation, adapt to novel environments, tolerate deleterious mutations, or evolve new functional capabilities, in the laboratory.

Computational biology and modeling
We use computational tools for phylogenetic analysis, sequence analysis, modeling, etc.

Universitat de València, 2020