CuAtDB

 Cu homeostasis factors in A. thaliana DataBase

Welcome to the Cu homeostasis factors in Arabidopsis thaliana DataBase.

To conduct a meaningful analysis of copper homeostasis in plants, it was necessary to compile a comprehensive list of all known and putative cuproproteins and Cu homeostasis factors from Arabidopsis and to organize the genes according to their gene families and functions. Since such comprehensive list was not available in the public databases, we have created the CuAtDB. The CuAtDB is operated by the Cu in Arabidopsis thaliana Laboratory (Universitat de València). Cuproproteins are classified on the basis of functionality, Cu coordination geometry and number of GTAC promoter motifs (Koch et al., 1997 and Andreini et al,. 2008). Asteriscs indicate ≥ 3 GTAC motifs in a ≤ 65 bp promoter region. The genes of the same families but not related to Cu are indicated in grey.

Links to references in TAIR, the Arabidopsis eFP Browser of The Bio-Array Resource for Plant Biology, ENSEMBL, KEGG and PubMed are provided.

The CuAtDB includes the results (log2) obtained in a microarray study to compare gene expression in 8 days old Arabidopsis seedlings grown on ½ MS plates (Cu deficiency) versus plants grown on the same medium supplemented with 10 µM copper sulphate (Cu excess). To identify genes differentially regulated by Cu, we have used a long-oligo array printed at the University of Arizona by Dr. David Galbraith (www.ag.arizona.edu/microarray/).

Biochemistry and Molecular Biology Department

Copper in Arabidopsis thaliana Laboratory