Traditional viral infection diagnostic techniques in the clinic are based on (RT-)PCR procedures, which are time consuming and require precise equipment and human resources, which preclude a rapid and massive intervention. Here, we will engineer a novel class of stand-alone biosystems aimed at diagnosing the presence of SARS-CoV-2. These biosystems consist of three reaction steps following sample collection without the need of sophisticated equipment: i) isothermal amplification of viral RNA, ii) CRISPR-based nucleic acid detection (working like an on-the-fly sequencing reaction), and iii) output disclosure by immunochromatographic assay.