The two-dimensional electrophoresis- Proteomics is still the technique most resolving and most employed in the proteomic analysis, as allow by a double separation in a 2D-PAGE gel based on the isoelectric point (pl) and the molecular weight (PM). However, given the drawbacks and limitations that presents, there is currently an intense activity in the search for alternative systems.

Among them, the DIGE technology (Differential In Gel Electrophoresis) sold by GE Healthcare, represents a clear advance in the comparative analysis of the differential expression of proteins, as it minimises the variability of gels, reduces the time of the analysis, and allows a very precise quantification of the expression profile.