Techniques

Assessing the carbohydrates malabsorption (lactose, fructose)-intestinal parasitosis relationship and the influence of the latter on child development.

Assessment of human functions: Effects of SMI in biomechanical parameters; evolutionary and temporal aspects.

Cell fusion trials based on viral membrane proteins.

Characterisation of the distribution of mutational effects on the biological efficacy of RNA viruses

Clinical assessment: extensive and detailed neuropsychological explorations of the clinical status in three assessment areas: neurocognitive; of social cognition and psychosocial functioning.

Consultancy in 3D Optical Microscopy and 3D Image and Display Techniques

Customised training on proteomics technologies

Detection, analysis and promotion of healthy life habits during the Covid-19 pandemic.

Determination of SARS-CoV-2 in environmental samples

Development in implementation of 3D image capture techniques based on the taking of multiple images with different perspective, the reconstruction of the original 3D scene

Development of epidemiological studies on food-transmissible parasites

Development of epidemiological studies on helminths and arthropods of health interest with the development of GISs to analyse the effect of climate change.

Development of innovative non-invasive microscopy techniques with high optical sectioning, such as confocal microscopy, structured illumination microscopy (SIM) or digital holographic microscopy (DHM)

Development of intervention programmes: Psychoeducational, for patients and relatives. Training and recovery of basic and social cognitive abilities.

Development of omics and exovesicle studies in human / animal helminths and their applicability in diagnosis, treatment and vaccination.

Elaboration of epidemiological studies on food-transmissible parasites

Estimation of the mutation rate of animal, plant and bacteriophage viruses (both RNA and DNA)

Evaluation of antiviral compounds on SARS-CoV-2 in the BSL3 (Biosafety level 3 Laboratory)

Evaluation of infection rates at the population level through rapid serological tests

Experimental model Aspiculuris treraptera in mice (CD1). Giardia intestinalis trophozoites of axenic cultivation

Use of animal experimental models (different strains of mice and rats. Animal Production Section, SCSIE). Isolation, analysis and characterisation of nucleic acids (DNA and RNA, especially miRNAs) and proteins via molecular biology techniques (restriction, PCR amplification, sequencing, dot-blot, southern and northern blot, western-blot). Bioinformatic analysis. Cloning and gene expression in heterologous systems (in bacteria, yeasts and superior eukaryotic cells, as well as in “cell-free” expression systems); production and purification of recombinant proteins. Production of polyclonal antibodies in rabbits. Cell cultures, SCSIE.

Genetic diagnosis: within the assessment of nervous system diseases, the genetic diagnosis service includes

Genomics-related services; Proteomics; Microscopy; Electron microscopy (SEM and TEM) and Confocal laser microscopy; Cell culture; Flow cytometry and Animal production

Genomics-related services; Proteomics; Microscopy; Electron microscopy (SEM and TEM) and Confocal laser microscopy; Cell culture; Flow cytometry and Animal production.

Heterologous expression of membrane proteins

High resolution 3D imaging and optical sectioning with Trinocular Confocal Fluorescence Microscope

Identification of transmission routes, gene introduction and spread, and drug resistance variants in viruses and bacteria

In-vitro and in-vitro estimation of mutation rates of different human viruses of high biomedical relevance, such as HIV-1 or Hepatitis C

Proteomic techniques and applications

Quantitative estimation of RNA virus mutation rates

Rapid Immunoglobulin detection (IgA secretory, IgGs and IgMs) for the early diagnosis of SARS-COV-2 via portable graphene biosensor.

Screening for antivirals and antibodies to SARS-CoV-2

Studies on the experimental evolution of oncolytic viruses for the development of therapeutic applications

Study of evolutionary processes using viruses as model organisms

Study of the evolution of complete genomes and genes in bacteria and viruses

Study of the optical dispersion of viruses, through micro-droplets and aerosols, caused by air currents generated by ventilation systems with the aim of optimising ventilation systems and closed spaces

Study of the spread of pathogenic microorganisms (bacteria and viruses) in human populations and natural reservoirs through genetic and genomic analysis for the purposes of epidemiological care and control

Technical advice on membrane protein biochemistry (overexpress, purification and structure-function relationship studies)

Technical advice on protein production in cell-free systems

Training for health professionals in the field of neurocognitive assessments

Course for Neurocognitive and Functional Assessment of patients with Serious Mental Illnesses.

Training in 3D Optical Microscopy Fundamentals

Training in Food Parasitology

Specialisation course in Food Parasitology since 2001.

Training in Parasitic Zoonosis

Training given to health workers by the research group on the main parasitic zoonosis since 2015.