The project aims to analyse NSCs, neurogenic niches and neurogenesis with age, as well as their involvement in AD. On the other hand, the role of GSK3 in the proliferation and differentiation processes of NSCs both in vitro and in vivo will be studied, as well as its regulation as a target.
Study of interrelationships between tumour neovascularisation, the presence of immunoregulatory cell populations and recurrence or progression in the tumour microenvironment.
Role of p38 alpha MAP kinase in the regulation of cytokinesis in hepatocytes.
The project deals with the application of cell therapy with genetically modified mesenchymal cells (episomal plasmid) to treat progression and repair neurological deterioration in the animal model of multiple sclerosis.
The neuroprotective role of bone marrow stem cells in a model of cerebral ischaemia will be analysed. To this end, the cells will be stained with iron and directed to the location of the injury by means of magnetic fields in order to increase the number of viable cells in the injured tissue.
Isolation and characterisation of extracellular vesicles (EVs) in different helminth parasites. Identification of molecules present in EVs. Analysis for the usefulness of EVs in helminthiasis control and other diseases (diagnosis, vaccination and treatment).
Our group has characterised a new important regulator of the Start transition: Whi7. Whi7 acts as a transcriptional repressor of the Start programme, collaborating with Whi5 in this function, so that, as occurs in mammals with the Rb family, the control of cell cycle initiation depends on the interplay between different repressors. The group's work aims to advance in the characterisation of the regulation and function of Whi7 and its comparison with Whi5 in different physiological conditions, which may help to understand how the action of different repressors is coordinated in the control of cell cycle initiation. In addition, the relationship between Whi7 and the protein kinase C pathway is being investigated. The fact that a member of the Rb family is mutated in almost all tumours further reinforces the importance of studying the role of these G1 repressors.
Assessment of biological effects of components of food and food ingredients: cytoprotection and/or cytotoxicity, markers of oxidative stress, inflammation and apoptosis, using cell cultures.
Yeast Pkc1 and the mammalian PKCd isoform share the same function of controlling the genomic integrity checkpoint. A parallel study in both organisms is proposed to characterise the molecular keys to this mechanism.
Yeast Pkc1 and the mammalian PKCd isoform share the same function of controlling the genomic integrity checkpoint. A parallel study in both organisms is proposed to characterise the molecular keys to this mechanism.
The overall project will identify and characterise neurogenic niches in different areas of the nervous system: the SVZ, the DG of the hippocampus, the third ventricle and the spinal cord. These niches will be analysed in different animal species from mouse, monkey to human.
The main objective of our line of research is the analysis of the cytopathological mechanisms involved in the fragmentation of the Golgi apparatus and in the alterations of intracellular trafficking in dopaminergic neurons in Parkinson's disease.
Analysis of biomarkers in minimally invasive samples (saliva, blood, cerebrospinal fluid, pleural fluid, etc.), with the aim of improving diagnosis, personalising treatments and monitoring patients more closely (detection of resistance and relapses).
Diagnostic, prognostic and/or predictive biomarkers: in lung cancer, head and neck cancer, colon cancer, breast cancer, melanoma, among others, through different omics approaches (genomics, transcriptomics, metabolomics).
Exosomes have been discussed in science as a possible route for long-distance communication, using vesicles. They contain proteins and nucleic acids. The project aims to learn more about these vesicles in blood, their morphology and molecular content from metastatic cancer.
Development of nanoparticles with functionalisation capacity for their application in bioimaging, anti-tumour therapy and regenerative therapy (Project developed by Vicente Herranz Pérez, post-doctoral researcher contracted by CIBERNED).
Study of the plasticity of the adult nervous system. Study of the estructural plasticity of interneurons in the adult brain and the role of polisialyc acid in this plasticity, the microcircuitry of the olfactory bulb and the hippocampus, both in control and in pathological (Down Syndrome, epilepsy...) conditions.
The group's work also focuses on the study of spatial regulatory mechanisms in cell cycle control, mechanisms that involve the control of the sub-cellular localisation of key proteins for progression in the cycle. In particular, the role of karyopherin Msn5 in the control of transcription factors (Swi6, Swi4, Mbp1, Swi1, Whi5) as well as Start cyclins (Cln1, Cln2) has been studied. Furthermore, determinants of cyclin functional specificity and the identification of new mechanisms controlling cyclin synthesis and degradation (Cln2, Clb2) are investigated.
The group's work also focuses on the study of spatial regulatory mechanisms in cell cycle control, mechanisms that involve the control of the subcellular localisation of key proteins for cell cycle progression. In particular, the role of the karyopherin Msn5 in the control of transcription factors (Swi6, Swi4, Mbp1, Swi5, Whi5) as well as Start cyclins (Cln1, Cln2) has been studied. Furthermore, determinants of cyclin functional specificity and the identification of new mechanisms controlling cyclin synthesis and degradation (Cln2, Clb2) are investigated.
Identification of new markers and molecular targets for the treatment of pulmonary fibrosis. Identification of molecular determinants of fibroblast-myofibroblast transition using omics technologies. Role of inflammation and stroma in the tumour microenvironment and lung cancer progression.
Identification of new markers associated with oxidative metabolism by mass spectrometry. Study of pathologies associated with oxidative stress and tissue hypoxia. Cellular and molecular mechanisms of the prevention of tissue damage in hypoxic-ischaemic neuropathies by means of natural and synthetic polyphenols.
Glaucoma research. Identification of genes and molecules involved in glaucoma. Cellular bases of glaucomatous disease. Application of omic sciences to the study of glaucoma.
In this line of work we aim to study the cellular and molecular mechanisms that regulate the correct balance between the production and specification of cerebral cortex cells by NSCs. We also aim to understand how alterations in these processes are related to neurodevelopmental diseases such as autism by studying murine models of these disorders.
This line aims to study basic epigenetic regulation (focusing on genomic imprinting) in NSCs under physiological conditions, and to identify novel epigenetic mechanisms that can potentially be modulated during reactivation therapies or tumour formation.
Our group has characterised a new important regulator of the Start transition: Whi7. Whi7 acts as a transcriptional repressor of the Start programme, collaborating with Whi5 in this function. So that, as occurs in mammals with the Rb family, the control of cell cycle initiation depends on the interplay between different repressors. The group's work aims to advance in the characterisation of the regulation and function of Whi7 and its comparison with Whi5 in different physiological conditions, which may help to understand how the action of different repressors is coordinated in the control of cell cycle initiation. In addition, the relationship between Whi7 and the protein kinase C pathway is being investigated. The fact that a member of the Rb family is mutated in almost all tumours further reinforces the importance of studying the role of these G1 repressors.
Genetic and cellular study of developmental processes in Drosophila, such as embryonic dorsal closure and the establishment of epithelial planar polarity, used as models of processes relevant to human health such as wound healing or cell migration processes.
Study of the molecular mechanisms responsible for the maintenance and correct functioning of NSCs in the adult brain. Specifically, we aim to address the study of how quiescence is regulated at the molecular level and how quiescence-activation responds to niche elements.
The aim is to analyse the myelination process during development, which are of vital importance for the induction of myelination/remyelination processes in the adulthood. In demyelination models, remyelination will be analysed using the acquired knowledge.
Recent studies have shown the presence of Lewy bodies within the enteric nervous system (ENS). Early onset gastrointestinal symptomatology prior to motor symptoms in Parkinson's disease has recently raised the possibility that lesions in the ENS may develop early in the course of the disease, prior to the appearance of cytopathology in the substantia nigra neurons, and thus the study of the ENS may help to understand the cytopathology of Parkinson's disease. These data and the ease of obtaining samples from patients by routine colonic biopsies have led to the use of the SNE as a study model in our line of research. Our main objective is to study the dopaminergic neurons of Meissner's and Auerbach's plexuses from both proximal and distal colon samples from a Parkinsonian rat model. The studies are carried out by means of high-resolution morphological analyses.
We will analyse the earliest effects that occur in the neurons of the spinal cord, as well as the role played by glia, with the aim of identifying the mechanisms that cause and are involved in the disease. On the other hand, a potential pathway of propagation between neurons will be studied.
Study of the alterations associated with or causing the degeneration of dopaminergic neurons and their relationship with ageing and associated conditioning factors. Studies of the effects of Parkinsonism on the behaviour of NSCs.
We test different materials including titanium prostheses or decellularised dental matrices for the regeneration of dental and peridental tissues. We also evaluate connective tissues such as dental pulp or periodontal ligament as sources of stem cells with different potentials. This line is funded by different projects financed in competitive public calls including IMPORTOX or NerBIO.
Characterisation and isolation of CSCs from tumour samples. Development of in vitro and in vivo models for the design of new therapeutic strategies to control the CSC population.
We are using several approaches to study human genetic diseases in Drosophila in order to dissect their pathogenesis pathways and identify biomarkers for diagnosis and/or progression, as well as to discover molecules with therapeutic potential.
