Room located inside the Cell Culture laboratory (NCB2), with its own equipment and higher level work protocols. Designed to work with viral vectors, GMOs or level 2 biological agents that require greater security.
NCB2+ containment level room with its own entrance lock (inside the Cell Culture laboratory) and access control, negative pressure, PVC walls in scotia moulding and HEPA air inlet and outlet filter.
It has complete equipment: biosafety cabin, compartment incubator, -80ºC freezer for the storage of biological agents, ultracentrifuge for their purification, JULI LG04 for the monitoring of infections with GFP, as well as the small equipment necessary for working with cell cultures.
The compartment incubator has six individual autoclavable polycarbonate chambers. They divide the incubator chamber isolating individual cell types or projects and offering greater protection.
ll this allows to work in this room in aseptic and isolated conditions for the adequate protection of the operator and the sample, to monitor cell growth, as well as to perform infections of viral constructs with GFP and to store biological agents within the same room.
The room also has CO2 detectors installed to ensure user safety.
Integrated resources in the NCB2+ room:
|
EQUIPMENT |
BRAND AND MODEL |
NUMBER |
INVENTORY CODE |
DESCRIPTION |
|
BIOLOGICAL SAFETY CABINET |
TELSTAR BIO-II ADVANCE 4 |
1 |
72-210580 |
Type IIA biosafety cabinet. It allows to protect the sample, the operator and the environment. 70% of air recirculation. 30% of air outdoor exit. It has EPA filters and germicide UV light for its disinfection. |
|
CO2 INCUBATOR |
THERMO FORMA FISHER Cell Locker |
1 |
72-219638 |
Compartment incubator for the maintenance of cell cultures. Optimal temperature, humidity and CO2 conditions for cell growth. |
|
AUTOMATIC CELLULAR IMAGING SYSTEM |
NanoEnTeck, JULI LG04 (GFP) |
1 |
Pending |
Green fluorescence microscope with the capacity to programme image acquisition over time. Allows automatic counting and determination of cell viability. |
|
CENTRIFUGE |
EPPENDORF 5810R |
1 |
Pending |
High-capacity, refrigerated centrifuge with a maximum speed of 5,500 rpm. |
|
MICRO-ULTRACENTRIFUGE |
HIMAC CS150FNX |
1 |
Pending |
Floor model ultracentrifuge with 3 rotors: S55A2, S50A and swing-out rotor S52ST |
|
-80ºC ULTRAFREEZER |
INFRICO |
1 |
Pending |
-80ºC freezer under table. |
|
4ºC REFRIGERATOR AND -20ºC FREEZER |
LIEBHERR |
1 and 1 |
--------------- |
Maintenance of users means, supplements and reagents. |
|
THERMOSTATIC BATHS |
RAYPA BAE-2 5l C/FP |
7 |
72-122440 |
Homogeneous temperature of 37°C for tempering means, reagents, samples, etc. |
|
tube SHAKER |
HEIDOLPH Reax-Top |
1 |
72-131619 |
Vortex tube shaker. |
|
SEMIAUTOMATIC outdoor DISTRIBUTION PLANT of gases with: 4 CO2 cylinders |
AIR LIQUIDE |
1 |
Pending |
Distribution plant that guarantees a continuous supply of CO2 to the room. |
|
Gas detection CONTROL UNIT |
OLDHAM MX32 with CO2 detector |
1 |
72-216080 |
Control of the CO2 level in the room with acoustic and light alarm. |
|
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Development of experiments with level 2+ biological agents. In particular:
- Generation of viral vectors.
- Infection of primary cultures and cell lines with viral vectors.
- Experimentation with GMOs.
ISO 9001:2015 certification
Annual validation of the Biosafety cabinets by an accredited external company
Annual review of incubators by an external company
To be an authorised user of the Cell Culture Service and in particular of the NCB2+ room. To do so, you must be informed about the project, the collaborators who will be working there, the use of biological agents, be familiar with the working rules in the room, take a guided tour by the section’s technicians and have previous experience working with cell cultures.
Access to the facilities is allowed wearing a lab coat, overshoes and a FFP2 mask for exclusive use in this room.
