Search result125 results

• Functional Integration of Neuronal Precursors in the Adult Murine Piriform Cortex

  Benedetti B, Dannehl D, König R, Coviello S, Kreutzer C, Zaunmair P, Jakubecova D, Weiger TM, Aigner L, Nacher J, Engelhardt M, Couillard-Després S

  (2020). Article

  Cereb Cortex.

  30(3):1499-1515

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• Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin

  Wakhloo D, Scharkowski F, Curto Y, Javed Butt U, Bansal V, Steixner-Kumar AA, Wüstefeld L, Rajput A, Arinrad S, Zillmann MR, Seelbach A, Hassouna I, Schneider K, Qadir Ibrahim A, Werner HB, Martens H, Miskowiak K, Wojcik SM, Bonn S, Nacher J, Nave KA, Ehrenreich H

  (2020). Article

  Nat Commun.

  11(1):1313

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• miR-7 Restores Phenotypes in Myotonic Dystrophy Muscle Cells by Repressing Hyperactivated Autophagy

  Sabater-Arcis M, Bargiela A, Furling D, Artero R - 2020 - Mol Ther Nucleic Acids

  (2020). Article

  Unstable CTG expansions in the 3' UTR of the DMPK gene are responsible for myotonic dystrophy type 1 (DM1) condition. Muscle dysfunction is one of the main contributors to DM1 mortality and morbidity. Pathways by which mutant DMPK trigger muscle defects, however, are not fully understood. We previously reported that miR-7 was downregulated in a DM1 Drosophila model and in biopsies from patients. Here, using DM1 and normal muscle cells, we investigated whether miR-7 contributes to the muscle phenotype by studying the consequences of replenishing or blocking miR-7, respectively. Restoration of miR-7 with agomiR-7 was sufficient to rescue DM1 myoblast fusion defects and myotube growth....

  Unstable CTG expansions in the 3' UTR of the DMPK gene are responsible for myotonic dystrophy type 1 (DM1) condition. Muscle dysfunction is one of the main contributors to DM1 mortality and morbidity. Pathways by which mutant DMPK trigger muscle defects, however, are not fully understood. We previously reported that miR-7 was downregulated in a DM1 Drosophila model and in biopsies from patients. Here, using DM1 and normal muscle cells, we investigated whether miR-7 contributes to the muscle phenotype by studying the consequences of replenishing or blocking miR-7, respectively. Restoration of miR-7 with agomiR-7 was sufficient to rescue DM1 myoblast fusion defects and myotube growth. Conversely, oligonucleotide-mediated blocking of miR-7 in normal myoblasts led to fusion and myotube growth defects. miR-7 was found to regulate autophagy and the ubiquitin-proteasome system in human muscle cells. Thus, low levels of miR-7 promoted both processes, and high levels of miR-7 repressed them. Furthermore, we uncovered that the mechanism by which miR-7 improves atrophy-related phenotypes is independent of MBNL1, thus suggesting that miR-7 acts downstream or in parallel to MBNL1. Collectively, these results highlight an unknown function for miR-7 in muscle dysfunction through autophagy- and atrophy-related pathways and support that restoration of miR-7 levels is a candidate therapeutic target for counteracting muscle dysfunction in DM1.

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  DOI: 10.1016/j.omtn.2019.11.012

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• RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

  Rosa-Téllez, S., Kanhonou R., Castellote-Bellés, C., Serrano, R., Alepuz, P., Ros, R.

  (2020). Article

  Agronomy.

  10:250

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• Drosophila SMN2 minigene reporter model identifies moxifloxacin as a candidate therapy for SMA

  Konieczny P, Artero R - 2020 - FASEB J

  (2020). Article

  Spinal muscular atrophy is a rare and fatal neuromuscular disorder caused by the loss of alpha motor neurons. The affected individuals have mutated the ubiquitously expressed SMN1 gene resulting in the loss or reduction in the survival motor neuron (SMN) protein levels. However, an almost identical paralog exists in humans: SMN2. Pharmacological activation of SMN2 exon 7 inclusion by small molecules or modified antisense oligonucleotides is a valid approach to treat SMA. Here we describe an in vivo SMN2 minigene reporter system in Drosophila motor neurons that serves as a cost-effective, feasible, and stringent primary screening model for identifying chemicals capable of crossing the...

  Spinal muscular atrophy is a rare and fatal neuromuscular disorder caused by the loss of alpha motor neurons. The affected individuals have mutated the ubiquitously expressed SMN1 gene resulting in the loss or reduction in the survival motor neuron (SMN) protein levels. However, an almost identical paralog exists in humans: SMN2. Pharmacological activation of SMN2 exon 7 inclusion by small molecules or modified antisense oligonucleotides is a valid approach to treat SMA. Here we describe an in vivo SMN2 minigene reporter system in Drosophila motor neurons that serves as a cost-effective, feasible, and stringent primary screening model for identifying chemicals capable of crossing the conserved Drosophila blood-brain barrier and modulating exon 7 inclusion. The model was used for the screening of 1100 drugs from the Prestwick Chemical Library, resulting in 2.45% hit rate. The most promising candidate drugs were validated in patient-derived fibroblasts where they proved to increase SMN protein levels. Among them, moxifloxacin modulated SMN2 splicing by promoting exon 7 inclusion. The recovery of SMN protein levels was confirmed by increased colocalization of nuclear gems with Cajal Bodies. Thus, a Drosophila-based drug screen allowed the discovery of an FDA-approved small molecule with the potential to become a novel therapy for SMA.

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  DOI: 10.1096/fj.201802554RRR

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• Pam3CSK4, a TLR2 ligand, induces differentiation of glioblastoma cells and confers susceptibility to temozolomide

  Megías, J., Martínez, A., San-Miguel, T., Gil, R., Muñoz, L., Albert, D., Carratalá, A., Gozalbo, D., López, C., Gil, M.L. and Cerdá, M.

  (2020). Article

  Investigational New Drugs.

  38(2), 299-310. Doi: 10.1007/s10637-019-00788-2. (2020)

  Category: “Pharmacology and Pharmacy”, Rank: 80/271 (Q2), IF: 3.525 (2019)

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• Glomus ibericum, Septoglomus mediterraneum and Funneliformis pilosus, three new species of arbuscular mycorrhizal fungi

  Guillén A; Serrano-Tamay F; Peris JB; Arrillaga I

  (2020). Article

  902553 - Mycologia.

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• Effect of elicitors on holm oak somatic embryo development and efficacy inducing tolerance to Phytophthora cinnamomi

  Morcillo M; Sales E; Ponce L; Guillén A; Segura J; Arrillaga I

  (2020). Article

  917079 - Scientific Reports (2020) 10:15166 |.

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• Diversispora valentina (Diversisporaceae), a new species of arbuscular mycorrhizal fungi in Mediterranean sand dunes of Spain

  Guillén A, Serrano-Tamay F; Peris JB; I Arrillaga I

  (2020). Article

  916710 - Phytotaxa.

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• Induction of radiata pine somatic embryogenesis at high temperatures provokes a long-term decrease in DNA methylation/hydroxymethylation and dif erential Eexpression of stress-related genes

  Castander-Olarieta A; Pereira C; Sales E; Meijón M; Arrillaga I; Cañal MJ, Goicoa T; Ugarte MD; Moncaleán P; Montalbán IA

  (2020). Article

  919156 - Plants-Basel.

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