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Description

Vascular tone depends on circulating vasoactive substances and neurotransmitters released in the vessel wall. Sympathetic tone is a fundamental component of vascular resistance that can be influenced by the presence of an intact endothelium, so it could be involved in pathologies where sympathetic tone is elevated, such as in local spasm and hypertension. From the beginning of the formation of the research group, one of the main objectives has been to lay the foundations for understanding the participation of the endothelium and NO in the nervous and hormonal regulation of blood flow in human arteries and veins in different regions. We have also been able to verify that endothelium and NO release do not have a common pattern in the various vascular beds studied. We have assessed, by indirect methods, basal and induced nitric oxide activity in different vascular beds. Knowledge of the basic mechanisms that relate endothelial factors to the perfusion of various human organs or vascular beds allows us to understand the impact that the endothelial-NO system can have on cardiovascular pathology in humans.

The endothelial modulatory role can be altered in various pathological conditions such as bronchial hyperresponsiveness, arterial hypertension, insulin resistance, hyperthyroidism and obesity among others. These pathologies share several risk factors and can often occur in association. For example, obesity is frequently associated with hypertension and increased sympathetic tone. It is therefore possible that the adrenergic stimulation of resistance arteries is modified as a consequence of an altered release of endothelial factors. The same may be true for the response to substances such as vasopressin, endothelin, thromboxane, angiotensin, which are directly involved in the regulation of circulation.

A few years ago we initiated in vitro studies of the effect of endogenous NO synthase inhibitors on endothelial nitric oxide release and their effects on the endothelium-dependent response to acetylcholine in patients with chronic renal failure and undergoing haemodialysis. We have also been able to verify the correlation between liver dysfunction and increased plasma ADMA concentration. In studies of patients with hepatorenal syndrome (HRS), we have shown elevated levels of ADMA and SDMA and correlations of SDMA with levels of renal impairment.

Our experience indicates that the contribution of the endothelium varies in different human vascular beds with varying pathology. Given the logical limitations of human experimentation, our studies in human arteries and veins are performed using in vitro techniques. These studies have many advantages from an experimental point of view, in particular the easy manipulation of vascular samples and the possibility of carrying out pharmacological studies with few limitations. For ethical reasons, studies cannot always be performed on human samples. In these cases we have resorted to different animal models. Recently, the study of vascular effects of new generation drugs has been added, including late sodium current blockers, levamisole interactions with cocaine, insulin degrading enzyme blockers, PPAR&#947 agonists and others.

Studies of vascular reactivity have been completed with analytical determinations by HPLC or colorimetric techniques of substances that can interfere with the endothelial nitric oxide-dependent vasodilator system (NOx, ADMA, SDMA, L-arginine among others) and determinations of protein and gene expression by western blot and PCR techniques.

Goals CT
  • Study of the mechanisms of vascular regulation in physiological and pathophysiological situations and with different pharmacological treatments.
Research lines

Vascular physiology, pathophysiology and pharmacology

Study of mechanisms regulating vascular tone.

Management
  • VILA SALINAS, JOSE M
  • PDI-Catedratic/a d'Universitat
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 Contactejose.m.salinas@uv.es
Members
  • ALDASORO CELAYA, MARTIN
  • PDI-Catedratic/a d'Universitat
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 Contactemartin.aldasoro@uv.es
  • MARTINEZ LEON, JUAN BAUSTISTA
  • PDI-Catedratic/a d'Universitat
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 Contactejuan.martinez-leon@uv.es
Collaborators
  • MAURICIO AVIÑO, MARIA DOLORES
  • Alumn.-Servei de Formacio Permanent
  • Vicedega/Vicedegana / Vicedirector/a Ets
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 Contactemamaua@alumni.uv.es
Scientific production by UV researcher
  • MAURICIO AVIÑO, MARIA DOLORES
    Alumn.-Servei de Formacio PermanentVicedega/Vicedegana / Vicedirector/a Ets
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CNAE

Research and experimental development on biotechnology

Associated structure
Contact group details
Vascular Physiology Research Group (InVas)

Blasco Ibáñez Campus

Av. Blasco Ibáñez, 15

46010 València (Valencia)

+34 963 864 644

Geolocation

jose.m.salinas@uv.es

Contact people
  • VILA SALINAS, JOSE M
  • PDI-Catedratic/a d'Universitat
View details
 Contactejose.m.salinas@uv.es