Self-assembly of amphipathic molecules with lipid and polypeptide characteristics, natural or synthetic. Characterisation of membrane structures of biological, biomedical and biotechnological interest, with special interest in pore, fusion and fission structures. Light-induced molecular changes in membranes, natural or synthetic. Design, implementation and applications of molecular switches in membranes.
- Design and characterisation of the interaction with lipid membranes of peptides and proteins with pore-forming and membrane remodelling activity.
- Description of mechanisms of pore formation, curvature changes and membrane fusion and fission processes.
- Molecular determinants and physico-chemical properties responsible at the sequence level for the membrane activity of polypeptide molecules.
- Redesign of polypeptide molecules to control their activity.
- Development of new experimental and theoretical methodology for the structural and functional study of membrane activities.
- Design and synthesis of membrane-active polypeptides
Design of new polypeptide sequences with membrane activity from native proteins and peptides.
Methodologies: Computational analysis of polypeptide structures at various levels. Chemical synthesis of peptides.
- Biophysical characterisation of polypeptide-membrane complexes in model systems
Study of the kinetic and thermodynamic properties of membrane-active complexes. Description of the structure of active complexes and their formation mechanisms.
Methodology: Infrared, UV-Vis, DC and fluorescence spectroscopies. Fluorescence microscopy. AFM nanoscopy. Langmuir monocaps. Molecular simulations. Theoretical diffusion models. Statistical analysis.
- SALGADO BENITO, JESUS
- PDI-Catedratic/a d'Universitat
- LORENZ FONFRIA, VICTOR ARMANDO
- PDI-Titular d'Universitat
- CALLE VELASQUEZ, MATEO
- Doctorand.
- ExpandirSALGADO BENITO, JESUSPDI-Catedratic/a d'Universitat
Burjassot/Paterna Campus
Science ParkC/ Catedrático José Beltrán, 2
46980 Paterna (Valencia)
- SALGADO BENITO, JESUS
- PDI-Catedratic/a d'Universitat
