- Small molecule structure elucidation: elucidation of connectivity, stereochemical configuration and conformation.
- Structural characterisation of nanoparticles and their interactions with substrates.
- Mobility studies and molecular dynamics.
- Monitoring of chemical processes: kinetics.
- Determination of association and molecular interaction processes.
- Studies at variable temperatures.
- Observation of a wide variety of magnetic cores: 19F, 195Pt, 133Cs, 11B, 31P, 6Li, 29Si, etc.
Structural characterisation of solid materials and immobilised substances on solids.
It enables the analysis of very diverse materials:
- Soil, wood, cellulose
- Ceramic materials
- Polymers
- Polymorphs, glass, metal and alloys
- Porous materials such as zeolites and aluminosilicates
- Nanoparticles
- Studies on solid organometallic complexes.
Small molecule structure elucidation: elucidation of connectivity, stereochemical configuration and conformation.
Structural characterisation of nanoparticles and their interactions with substrates.
Mobility studies and molecular dynamics.
Monitoring of chemical processes: kinetics.
Determination of association and molecular interaction processes.
Studies at variable temperatures.
Mixture analysis, component quantification and determination.
Studies on molecular dissemination, size and mobility.
Studies on chemical reaction mechanisms, characterisation of reaction intermediates, acquisition of kinetic and thermodynamic reaction parameters.
Studies on molecular interactions, association processes determination and host-guest affinity molecular interaction, enzyme-substrate molecular recognition.
Electrophoresis, in situ PCR, RT PCR, quantitative PCR, staining and detection methods, transfer analysis (western, southern...), recombinant DNA technology (DNA extraction, enzymatic treatment, DNA transfer ...), flow cytometry and "FACS" (Fluorescence-Activated Cell Sorter), genomic amplification (WGA, whole genome amplification), sample treatment for optical and electronic microscopy, inclusion, Immunohistochemistry, FISH.
Galaxy Badger, UGene, Artemis: Genome Browser, ACT, ClustalX, MrBayes, Mafft, RAxML, GS De Novo Assembler, GPRo, MIRA, Velvet, AphidBase, Staden, Unix Desktop Environment Project (UDE).
Analysis of genome and proteomics sequencing data, creation of phylogenetic trees, computer programming, BLASTO, study of biochemical networks
The bright-field microscope allows us to view stained or naturally pigmented samples that are highly contrasted. The source of illumination is white light. The components of the sample are visualised thanks to the differences in contrast between them and the surrounding medium.
The equipment allows DNA sequencing by the Sanger method and fragment size determination.
Cell Culture laboratory of Biological Containment Level 2 (BCL2). It has several rooms: Cell Lines Room, Primary Cultures Room, Hypoxia Room and Viral Vectors Room (BCL2+), with 10 biosafety cabinets, 6 incubators and all the necessary equipment to work with eukaryotic cell cultures.
Prepared and controlled by technicians so the user just focuses on his experiment.
Cell Separator equipped with 6 lasers, biosafety cabinet and aerosol extraction. It allows, firstly, the rapid measurement of morphological and functional parameters of a large number of individual cells suspended in liquid medium. For this, cells are initially labelled with fluorochrome-conjugated antibodies, or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
In addition, after analysis, it is possible to separate and collect subpopulations of interest from the sample for further studies.
In vitro transcription/translation systems.
The confocal microscope allows us to visualize images with different fluorescent labelling, therefore obtaining images of great sharpness and quality because the pictures earned are not contaminated by light emitted outside the focal plane. Thanks to this confocal characteristic, we can perform three-dimensional reconstructions from optical sections.
Fully equipped laboratory for sample preparation, acquisition, separation and analysis by flow cytometry.
The variety of fluorescent proteins and multicolour probes that have been developed allows virtually any molecule to be tagged. The ability to visualise protein dynamics in vesicles, organelles, cells and tissues has provided new information about the functioning of cells in both healthy and diseased situations. This information includes the spatio-temporal dynamics of processes, such as mitosis; studies on the dynamics of ions, such as Ca+₂ and changes in the cytoskeleton.
Ability to address the DNA sequencing of genetic material from any organism using both traditional sequencing techniques (Sanger sequencing) as Next-Generation Sequencing (NGS).
3 laboratories, 9 offices, 12 jobs for predoctoral researchers, server room (6 jobs for researchers and 4 offices).
Confocal microscope mounted on a motorised inverted IX81 which includes the following lenses: 10x, 20x, 40x (oil), 60x (oil), 60x (water). This equipment’s excitation lines are: 405nm, 488nm, 515nm, 559nm, 594nm y 635nm. Moreover, the equipment has an incubation system, which makes it the appropriate equipment for live-cell work.
Multiphoton confocal microscope mounted on a motorised BX61WI upright microscope equipped with a XPLN25xWMP water immersion lens exclusive to multiphoton microscopy, with a numerical aperture of 1.05 and a working distance of 2mm. It has a Mai-Tai HP DeepSee pulsed tunable laser (Spectra Physics) in the 690 to 1040nm range with an average power of 2.0 W. The infrared element of this laser allows for further penetration in the sample and causes less cell damage. The signal is picked up by four detectors covering the whole visible range (420-500/515-580/590-650/660-740).
Fully motorised standard confocal equipment allowing for programmed acquisitions on several predefined areas. It has a dry 10x (NA 0,4) lens and a 60x (NA 1,2) water immersion one. The excitation lines are: 405nm, 473nm, 559nm y 635nm. This equipment is suited for live-cell experiments as it includes an incubator with temperature control.
A 5-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in a liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
A three-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
Fluorescence is one of the most widely used physical phenomena in biological and analytical microscopy, mainly because of its high degree of sensitivity and specificity. Fluorescence microscopy allows users to determine the distribution of a single molecule, its quantity and its location within a cell.
Fluorescence microscopes used in research applications are based on a series of optical filters. The filters are usually inserted in a filter block. While the excitation filter selects the wavelengths that excite a particular fluorophore within the sample, the emission filter acts as a kind of quality control, as it only allows the wavelengths of interest emitted by the fluorophore to pass through.
Platform to carry out transcriptomic studies in the single cell field, allowing the study of transcriptomic and phenotypic variability among thousands of cells based on single cell RNA.
High content screening analysis equipment via automatic image capture for the study of live or fixed cell cultures, in plates or slides. It includes four long-distance lens: 4x, 10x, 20x y 40x. It also includes an incubator with temperature control and an automatic reagent dispenser.
Equipment that quantitatively determines the concentrations of one, several or many substances in a biological (serum, plasma, tissue or urine), food or environmental sample. This is achieved by chromatographic separation of the compounds present in the matrix to be studied, either by liquid chromatography or gas chromatography and their subsequent nebulisation with fragmentation to detect the components according to their molecular weight.
Refrigerated centrifuges, micropipettes, magnifiers, PCR Lifecycler, Orbital vacuum pumps, exhaust hoods and laminar flows, vortex, MiniSpin, cooker, electrophoresis buckets, Qubit 2.0, NanoDrop 1000, gas burner, photoperiod insect breeding chambers, scale, photo documentation equipment, thermal cyclers, freezers, refrigerators.
The Massarray platform is responsible for the research study of biological samples through the fine mapping of DNA sequences and the analysis of methylation phenomena. With this equipment, dozens of DNA variants such as SNPs, insertions or deletions can be analysed in hundreds of samples in a short time with a high degree of precision and sensitivity. It is also possible to analyse somatic mutations such as those present in samples of cancerous origin. Finally, the degree of methylation of individual CpGs in short DNA sequences can be quantitatively quantified.
Massive DNA sequencing equipment based on sequencing by synthesis, using nucleotides labelled with fluorophores of different colours, and capturing images of the emitted fluorescence.
High-throughput sequencing equipment that allows obtaining short sequences of up to 300 bp.
High-throughput sequencing equipment that allows obtaining sequences with an average length of more than 30 kb.
Ultracentrifuge for small volumes with 3 rotors: S55A2, S50A and swing-out rotor S52ST.
Molecular biology equipment: 2 thermal cyclers. 1 Gradient thermal cycler. Image Analyser for agarose gels. QIAxcel System for nucleic acid analysis. Laminar flow cabinet for bacteria culture and phage work. 2 colony counters.
Flow cytometry, qRT-PCR, Western Blot, Big-Data and primary cell culture techniques.
Room located inside the Cell Culture laboratory (NCB2), with its own equipment and higher level work protocols. Designed to work with viral vectors, GMOs or level 2 biological agents that require greater security.
Equipment: 3 laminar flow cabinets for cell culture. 3 CO2 incubators. 1 Hypoxia workstation. Inverted microscope. Micromanipulator. -80ºC cabinet. Autoclave. Freezer.
Application of molecular phylogenetic inference methods to sequence data from ultra-sequencing to the determination of new genomes.
Real-time PCR is a technique that combines amplification and detection in a single step by correlating the PCR product of each cycle with a fluorescence intensity signal. They consist of a thermal cycler coupled to an optical system, which monitors the signal of the fluorophores used to detect the amplified product. Because the fluorescence of the fluorophores increases as the product is amplified, the amplification and detection processes are combined in a single step.
Equipment for the realisation of quantitative PCR tests.
Equipment for the realisation of digital quantitative PCR tests.
Computer cluster for scientific calculation and data storage.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
The equipment has a cell separation system allowing the physical separation of a given population with different levels of purity. It allows separation under sterile conditions.
UV-visible absorbance spectroscopy, circular dichroism and fluorescence.
Super-resolution confocal microscope LCS 980 has the following objectives: 10x, 20x, 25x (oil), 40x (water) and 63x (oil), 60x. The excitation lines present in this equipment are: 405nm, 488nm, 561nm and 639nm. This equipment also has an incubation system, which makes it suitable for working with live cells. This equipment incorporates the Airyscan 2 and Elyra 7 super-resolution systems.
High-contrast 120Kv transmission electron microscope with 20 Mpx CMOS EMSIS XAROSA digital camera.
High contrast 100 Kv transmission electron microscope with 8 Mpx AMT digital camera.
Breeding and maintenance of insects, dissection of their central nervous system
The Field Emission Scanning Electron Microscope with focused ion beam (FIB). Counts with the EDX microanalysis, cryo option for the observation of frozen samples and variable pressure.
This is an O2-CO2 gas analyser with air flow and two-animal switch. It contains: a software platform for metabolism, "respiratory metabolism" extension of the Metabolism software, single line Treadmill for mouse with shock, Calorimetry Runner for the treadmill, Rat/Mouse tray, Two Calorimetry lids for food and drink rats, Calorimetry Feeder/Drink, 100mL bottle for Oxylet system, Rat grid floor, Mouse grid floor. This equipment has been supervised by the specialist technician Javier Cuenca.
