Morphological, morphometric and molecular protozoa diagnosis; diagnosis of helminths and arthropod parasites of human and animal nature. Epidemiological modelling of human and animal parasitic diseases via creation of Geographical Information Systems (GIS).
Electrophoresis, in situ PCR, RT PCR, quantitative PCR, staining and detection methods, transfer analysis (western, southern...), recombinant DNA technology (DNA extraction, enzymatic treatment, DNA transfer ...), flow cytometry and "FACS" (Fluorescence-Activated Cell Sorter), genomic amplification (WGA, whole genome amplification), sample treatment for optical and electronic microscopy, inclusion, Immunohistochemistry, FISH.
Lab spaces disposition, equipped with the necessary inventory and fungible material to finalise investigations in detailed lines. Laboratories equipped with ELISA readers, mono- and two-dimensional electrophoresis systems, ChemiDoc XRS Bio-Rad analysis and documentation system, normal and real-time PCR thermocyclers (real-time PCR CFX96 Touch system; Bio-Rad). Electroporator, incubators, coolers, -20 and -80 freezers. Semi-wet transfer equipment. Refrigerated centrifuges with fixed and oscillating rotors, for various tube sizes. Floor and tabletop ultracentrifuges. Autoclaves, water purification systems. Optic and fluorescence microscopes. Staining trays and specific staining reagents. ELISA plate reader and real-time thermocycler.
Galaxy Badger, UGene, Artemis: Genome Browser, ACT, ClustalX, MrBayes, Mafft, RAxML, GS De Novo Assembler, GPRo, MIRA, Velvet, AphidBase, Staden, Unix Desktop Environment Project (UDE).
Analysis of genome and proteomics sequencing data, creation of phylogenetic trees, computer programming, BLASTO, study of biochemical networks
Ability to address the DNA sequencing of genetic material from any organism using both traditional sequencing techniques (Sanger sequencing) as Next-Generation Sequencing (NGS).
3 laboratories, 9 offices, 12 jobs for predoctoral researchers, server room (6 jobs for researchers and 4 offices).
Refrigerated centrifuges, micropipettes, magnifiers, PCR Lifecycler, Orbital vacuum pumps, exhaust hoods and laminar flows, vortex, MiniSpin, cooker, electrophoresis buckets, Qubit 2.0, NanoDrop 1000, gas burner, photoperiod insect breeding chambers, scale, photo documentation equipment, thermal cyclers, freezers, refrigerators.
Massive DNA sequencing equipment based on sequencing by synthesis, using nucleotides labelled with fluorophores of different colours, and capturing images of the emitted fluorescence.
NIKON DXM 1200C Digital Camera. NIKON E400 microscope. Progres CT3 USB Digital Camera; NIKON SMZ 745T Magnifier.
Molecular biology equipment: 2 thermal cyclers. 1 Gradient thermal cycler. Image Analyser for agarose gels. QIAxcel System for nucleic acid analysis. Laminar flow cabinet for bacteria culture and phage work. 2 colony counters.
Equipment: 3 laminar flow cabinets for cell culture. 3 CO2 incubators. 1 Hypoxia workstation. Inverted microscope. Micromanipulator. -80ºC cabinet. Autoclave. Freezer.
Phage collection.
Application of molecular phylogenetic inference methods to sequence data from ultra-sequencing to the determination of new genomes.
Real-time PCR is a technique that combines amplification and detection in a single step by correlating the PCR product of each cycle with a fluorescence intensity signal. They consist of a thermal cycler coupled to an optical system, which monitors the signal of the fluorophores used to detect the amplified product. Because the fluorescence of the fluorophores increases as the product is amplified, the amplification and detection processes are combined in a single step.
Chemi-Doc XRS gels documentation system (Bio-Rad). Floor and tabletop ultracentrifuges. Real-time PCR CFX96 Touch System (Bio-Rad). Fluorescence microscope. Image contrast microscope and digital video camera-imaging system. ELISA microplate readers. Mono and two-dimensional electrophoresis systems. Refrigerated centrifuges, freezers and coolers; orbital incubators; electroporator; sonicator; autoclaves and water purification systems.
Breeding and maintenance of insects, dissection of their central nervous system
