Type: Equipment
A 5-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in a liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
Flow cytometer analyser with 5 lasers (375nm, 405nm, 488nm, 561nm and 640nm).
Detection of 20 parameters (18 fluorescence and 2 light scattering).
Uses FACSDiva 8 software for acquisition and analysis.
|
FILTER SETTINGS FORTESSA |
||
|
|
|
|
|
DETECTOR BLUE 488nm |
MIRROR |
FILTER |
|
A |
655 LP |
695/40 |
|
B |
505 LP |
530/30 |
|
C |
---- |
488/10 |
|
DETECTOR Yellow-green 561nm |
MIRROR |
FILTER |
|
A |
750 LP |
780/60 |
|
B |
635 LP |
670/30 |
|
C |
600 LP |
610/20 |
|
D |
---- |
586/15 |
|
DETECTOR Red 640nm |
MIRROR |
FILTER |
|
A |
750 LP |
780/60 |
|
B |
690 LP |
730/45 |
|
C |
---- |
670/14 |
|
DETECTOR UV 355nm |
MIRROR |
FILTER |
|
A |
690 LP |
740/35 |
|
B |
410 LP |
450/40 |
|
C |
---- |
379/28 |
|
DETECTOR Violet 405nm |
MIRROR |
FILTER |
|
A |
750 LP |
780/60 |
|
B |
685 LP |
710/50 |
|
C |
630 LP |
660/20 |
|
D |
595 LP |
610/20 |
|
E |
505 LP |
525/50 |
|
F |
--- |
450/50 |
In addition, the laboratory has the following auxiliary equipment:
|
EQUIPMENT |
BRAND AND MODEL |
NUMBER |
INVENTORY CODE |
DESCRIPTION |
|
BIOLOGICAL SAFETY CABINET |
TELSTAR BIO-II ADVANCE 3 |
1 |
72-210580 |
Biosafety cabinet type IIA, for sample preparation. |
|
CENTRIFUGE |
EPPENDORF 5702 |
1 |
72-217839 |
Centrifuge for 5, 15 and 50 ml tubes with a maximum speed of 5.500 rpm. |
|
THERBLOCK |
FISHERBRAND |
1 |
72-214958 |
Digital dry bath for 1.5ml tubes |
|
REFRIGERATOR AND FREEZER |
SANYO MPR 414F |
1 |
72-163852 |
COMBI with 340L refrigerator and 82L freezer for reagent storage. |
|
TUBE SHAKER |
HEIDOLPH Reax-Top |
1 |
72-143423 |
Vortex for sample and reagent agitation. |
|
||||
Detection of cell subpopulations (immunophenotyping).
Apoptosis, viability and cell cycle assays.
Functional assays (metabolism, oxidation, proliferation, intracellular calcium, pH, etc.).
Studies at subcellular level (mitochondria, etc.).
Studies with prokaryotic cells, yeast and algae.
Absolute quantifications using reference beads (trucount tubes).
Advice to potential users on the applicability of this technique to their research.
ISO 9001:2015 Certification
Annual validation of the biosafety cabinets by an accredited external company.
Annual review of the cytometer by an external company
To be an authorised user of the Flow Cytometry Service. To this end, you must inform about the project, the collaborators who will be working on it, the use of biological agents and be familiar with the working rules. To become an independent user, it is necessary to receive prior training from the technicians of the section.
- Norte Pinto, Sandra Cristina
- PIT-Investigacio Escala Tecnica Mitjana
