Platform to carry out transcriptomic studies in the single cell field, allowing the study of transcriptomic and phenotypic variability among thousands of cells based on single cell RNA.

The Chromium Controller is based on 10X Genomics' NEXT GEM technology, in which cells are encapsulated together with Gel Beads in vesicles called Gel Beads in Emulsion (GEMs). These act as individual reaction vesicles where the sample is labelled. This labelling is achieved because each Bead contains millions of oligonucleotides labelled with barcode sequences. The platform uses microfluidic technology to encapsulate, process and analyse hundreds to millions of cells. It uses a single cell encapsulation protocol to encapsulate single cells into individual beads and label each individual cell with a molecular barcode and the necessary reagents for target identification and amplification. The device is capable of encapsulating and processing, in each run, up to 80,000 single cells with a capture efficiency of up to 65%. The single cell capture technology has a doublet capture rate (2 cells encapsulated at a time) of less than 1% per 1000 cells. The microfluidic chip using Chromium is compatible with a wide range of eukaryotic cell sizes and cell types (adherent cell cultures, suspension cell cultures, tissues, whole blood, etc.). The labelled products are pooled for downstream reactions to create gen libraries compatible with massive short read sequencing kits such as Illumina kits.