High-performance Quadrupole Time-of-Flight (QTOF) mass spectrometer for accurate mass and high-resolution analysis. This system is essential for identifying unknown compounds and performing advanced quantification studies in complex samples.

• Sensitivity and Resolution:

  • TOF/MS Sensitivity: in positive ESI mode for a 1 pg on-column reserpine injection, producing a S/N ratio > 2500.

  • MS/MS Sensitivity: in positive ESI mode for a 50 fg on-column reserpine injection, producing a S/N ratio > 400.

  • TOF/MS Sensitivity with Twin Sprayer Probe: 1 pg on-column reserpine injection produces S/N > 750.

  • MS Resolution: higher than 42,000 (FWHM) m/z 956.

• Performance and Acquisition:

  • MS/MS Scan Speed: up to 133 Hz (133 MS/MS spectra per second).

  • MS/MS duty cycle: ≥ 90% for TOF MS/MS acquisition.

  • Scanning modes: Full scan TOF MS, Full scan TOF MS/MS, MRMHR, SWATH (DIA), IDA (DDA).

  • Linear dynamic range: better than 5 orders of magnitude without signal loss.

• Accuracy and Ranges:

  • Internal mass accuracy: less than 0.2 ppm absolute error.

  • External mass accuracy: less than 2 ppm RMS over 12 hours of LC-MS operation.

  • Mass range: up to 40 kDa in TOF; Precursor Ion 5-2,250 m/z (5-40,000 m/z).

  • Workflow flow range: from 5 μL/min to 3 mL/min.

  • Fragmentation modes: CID collision cell and EAD cell.

The use of the equipment will only be carried out by the technical staff of the section and authorized users for the operation of the equipment.