Type: Equipment
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
Flow cytometer analyzer. The equipment consists of 3 lasers. The configuration of lasers and filters is as follows:
- Blue Laser 488nm contains 2 detectors for Forward Scatter (FSC) and Side Scatter (SSC) cell morphology and 4 detectors 507LP 527/32 nm; 560 LP 586/42 nm; 665LP 700/54 nm; 752LP 783/56 nm for fluorescence.
- Red Laser 640nm contains 2 detectors 610/10 nm; 752LP 783/56 nm for fluorescence.
- Violet Laser 405 nm contains 2 detectors 448/45 nm; 500LP 528/45 for fluorescence.
The cytometer has a manual sample collection. The sampling software is BDFACsuite.
- Study of cell death/apoptosis/necrosis.
- Study of reactive oxygen and nitrogen species generation in human and mouse models.
- Study of fluorescent proteins in different human cell lines.
- Cell phenotype of leukocyte populations, erythrocytes, platelets in human and mouse models.
- Study of exosomes in cell cultures and plasma samples.
- Study of cell physiology. Plasma membrane potential, intracellular calcium levels, mitochondrial membrane potential, cell cycle in intact cells and isolated nuclei.
- Study of bacterial physiology. Cell death, generation of reactive oxygen species, plasma membrane potential.
- Study of fluorescent molecules bound to certain cell receptors for identification of a specific cell type.
ISO 9001
Equipment for sample determination. To use it, the user must make a request by e-mail or by telephone to the Cytometry Service of the UCIM. There are two ways of use, with or without a technician (self-service). Users who wish to acquire samples in self-service mode must undergo a prior evaluation by the head of the Service.
- Herrera Martin, Guadalupe
- PIT-Investigacio Escala Tecnica Superior
- Escrig Domenech, Aaron
- PDI-Associat/Da Universitari/A
- PAS-E.T.B. Investigacio
