The bright-field microscope allows us to view stained or naturally pigmented samples that are highly contrasted. The source of illumination is white light. The components of the sample are visualised thanks to the differences in contrast between them and the surrounding medium.
Cell Culture laboratory of Biological Containment Level 2 (BCL2). It has several rooms: Cell Lines Room, Primary Cultures Room, Hypoxia Room and Viral Vectors Room (BCL2+), with 10 biosafety cabinets, 6 incubators and all the necessary equipment to work with eukaryotic cell cultures.
Prepared and controlled by technicians so the user just focuses on his experiment.
Cell Separator equipped with 6 lasers, biosafety cabinet and aerosol extraction. It allows, firstly, the rapid measurement of morphological and functional parameters of a large number of individual cells suspended in liquid medium. For this, cells are initially labelled with fluorochrome-conjugated antibodies, or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
In addition, after analysis, it is possible to separate and collect subpopulations of interest from the sample for further studies.
The confocal microscope allows us to visualize images with different fluorescent labelling, therefore obtaining images of great sharpness and quality because the pictures earned are not contaminated by light emitted outside the focal plane. Thanks to this confocal characteristic, we can perform three-dimensional reconstructions from optical sections.
Fully equipped laboratory for sample preparation, acquisition, separation and analysis by flow cytometry.
The variety of fluorescent proteins and multicolour probes that have been developed allows virtually any molecule to be tagged. The ability to visualise protein dynamics in vesicles, organelles, cells and tissues has provided new information about the functioning of cells in both healthy and diseased situations. This information includes the spatio-temporal dynamics of processes, such as mitosis; studies on the dynamics of ions, such as Ca+₂ and changes in the cytoskeleton.
Ability to address the DNA sequencing of genetic material from any organism using both traditional sequencing techniques (Sanger sequencing) as Next-Generation Sequencing (NGS).
A 5-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in a liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
A three-laser analysing cytometer for rapid measurement of morphological and functional parameters of large numbers of single cells suspended in liquid medium. For this purpose, cells are initially labelled with fluorochrome-conjugated antibodies or fluorescent probes that bind to proteins located on the cell surface or inside the cell. The scattered light and fluorescence emitted by the markers as the cells are passed one by one through the different lasers allows simultaneous measurement of structural features and various parameters in each of these cells.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
Fluorescence is one of the most widely used physical phenomena in biological and analytical microscopy, mainly because of its high degree of sensitivity and specificity. Fluorescence microscopy allows users to determine the distribution of a single molecule, its quantity and its location within a cell.
Fluorescence microscopes used in research applications are based on a series of optical filters. The filters are usually inserted in a filter block. While the excitation filter selects the wavelengths that excite a particular fluorophore within the sample, the emission filter acts as a kind of quality control, as it only allows the wavelengths of interest emitted by the fluorophore to pass through.
The Massarray platform is responsible for the research study of biological samples through the fine mapping of DNA sequences and the analysis of methylation phenomena. With this equipment, dozens of DNA variants such as SNPs, insertions or deletions can be analysed in hundreds of samples in a short time with a high degree of precision and sensitivity. It is also possible to analyse somatic mutations such as those present in samples of cancerous origin. Finally, the degree of methylation of individual CpGs in short DNA sequences can be quantitatively quantified.
Massive DNA sequencing equipment based on sequencing by synthesis, using nucleotides labelled with fluorophores of different colours, and capturing images of the emitted fluorescence.
Ultracentrifuge for small volumes with 3 rotors: S55A2, S50A and swing-out rotor S52ST.
Molecular biology equipment: 2 thermal cyclers. 1 Gradient thermal cycler. Image Analyser for agarose gels. QIAxcel System for nucleic acid analysis. Laminar flow cabinet for bacteria culture and phage work. 2 colony counters.
Room located inside the Cell Culture laboratory (NCB2), with its own equipment and higher level work protocols. Designed to work with viral vectors, GMOs or level 2 biological agents that require greater security.
Equipment: 3 laminar flow cabinets for cell culture. 3 CO2 incubators. 1 Hypoxia workstation. Inverted microscope. Micromanipulator. -80ºC cabinet. Autoclave. Freezer.
Application of molecular phylogenetic inference methods to sequence data from ultra-sequencing to the determination of new genomes.
Real-time PCR is a technique that combines amplification and detection in a single step by correlating the PCR product of each cycle with a fluorescence intensity signal. They consist of a thermal cycler coupled to an optical system, which monitors the signal of the fluorophores used to detect the amplified product. Because the fluorescence of the fluorophores increases as the product is amplified, the amplification and detection processes are combined in a single step.
Equipment that determines and quantifies the fluorescence of fluorescent probes attached to different parts of both eukaryotic and prokaryotic cells, organelles, proteins. The emitted fluorescence is collected on different filters limited to a certain wavelength. Fluorescence quantification is associated with the cell parameter being measured, generating quantitative results such as fluorescence intensity, percentage of cell populations, cell count/ML.
The equipment has a cell separation system allowing the physical separation of a given population with different levels of purity. It allows separation under sterile conditions.
