Innovation in arts and cultural sectors is an emerging study. Exploring and identifying driving factors of innovation by arts and cultural organizations can help to understand the implications for innovation management and thus, strengthening the competitiveness of arts and cultural sectors. But the existing literature on this topic usually is subject to some limitations due to the shortage of empirical evidence and overemphasis on technological innovation. To overcome it, this article explored evidence-based determinants of innovation in museums organizations, based on survey data and statistical approach; furthermore, it also extended the scope of innovation from technological to cultural and organizational dimensions. Our findings provide empirical evidence for existing theoretical propositions—some propositions are supported and the others are not. Based on these conclusions, some implications are developed for innovation management in museum organizations.