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  • High density MicroArrays on Blu-​ray discs for massive screening

    Arnandis-Chover, Tania; Morais, Sergi; Gonzalez-Martinez, Miguel Angel; Puchades, Rosa; Maquieira, Angel

    (2014). Article

    Biosensors and Bioelectronics.

    The potential and the capabilities of Blu-​ray technol. (disks and drives) for massive screening applications are presented. High d. microarrays are fabricated onto a Blu-​ray disk and applied for the detn. of microcystin residues and pathogenic microorganisms. Specific probes were physisorbed onto the BD surface and the biorecognition event was displayed using labeled secondary antibody soln. and subsequent signal amplification. The attenuation of the reflected light caused by the reaction product is detected by the Blu-​ray drive and inversely correlated with analyte concn. BDs preserve the optical properties according to Blu-​ray specifications, ensuring max. accuracy and sensitivity...

    The potential and the capabilities of Blu-​ray technol. (disks and drives) for massive screening applications are presented. High d. microarrays are fabricated onto a Blu-​ray disk and applied for the detn. of microcystin residues and pathogenic microorganisms. Specific probes were physisorbed onto the BD surface and the biorecognition event was displayed using labeled secondary antibody soln. and subsequent signal amplification. The attenuation of the reflected light caused by the reaction product is detected by the Blu-​ray drive and inversely correlated with analyte concn. BDs preserve the optical properties according to Blu-​ray specifications, ensuring max. accuracy and sensitivity of the drive during disk scanning. Detection limits of 0.4 μg​/L for microcystin LR and 100 and 101 cfu​/mL for Salmonella typhimurium and Cronobacter sakazakii resp., were achieved, improving considerably the DVD performances and reaching similar sensitivity as real-​time quant. PCR. Blu-​ray technol. adapted to the anal. of high d. arrays highlights the enormous capabilities (high sensitivity, speed-​scanning, optical resoln., portability) for point-​of-​care settings, diagnostics, and high-​throughput screening applications.

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    Volume 51
    Pages 109-114

     

    DOI: 10.1016/j.bios.2013.07.045
    ISSN: 0956-5663
  • Total Analysis Systems with Thermochromic Etching Discs Technology

    Avellà Oliver, José Miguel; Morais Ezquerro, Sergi Beñat; Carrascosa Rubio, Javier; Puchades Pla, Rosa; Maquieira Catalá, Ángel

    (2014). Article

    ANALYTICAL CHEMISTRY.

    Volumen: 86 Páginas: 12037-12046

  • Silicon nanopillar arrays with SiO2 overlayer for biosensing application

    Dev Choudhury, B.; Casquel, Rafael; Bañuls Polo, Mª José; Sanza, F.J.; Laguna, M.F.; Holgado, M.; Puchades Pla, Rosa; Maquieira Catalá, Ángel; Angulo, Carlos; Anand, S.

    (2014). Article

    OPTICAL MATERIALS. No.7

    Número: 7  Volumen: 4  Páginas: 1345-1354

  • Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis

    Santiago Felipe, Sara; Tortajada Genaro, Luis Antonio; Puchades Pla, Rosa; Maquieira Catalá, Ángel

    (2014). Article

    ANALYTICA CHIMICA ACTA.

    Volumen: 811  Páginas: 81-87

  • One-pot isothermal DNA amplification Hybridisation and detection by a disc-based method

    Santiago Felipe, Sara; Tortajada Genaro, Luis Antonio; Morais Ezquerro, Sergi Beñat; Puchades Pla, Rosa; Maquieira Catalá, Ángel

    (2014). Article

    SENSORS AND ACTUATORS B-CHEMICAL.

    Volumen: 204  Páginas: 273- 281

  • Direct covalent attachment of DNA microarrays by rapid thiol-​ene "click" chemistry

    Escorihuela, Jorge; Banuls, Maria-Jose; Grijalvo, Santiago; Eritja, Ramon; Puchades, Rosa; Maquieira, Angel

    (2014). Article

    Bioconjugate Chemistry. No.3

    A rapid strategy for the covalent immobilization of DNA onto silicon-​based materials using the UV-​initiated radical thiol-​ene reaction is presented in this study. Following this approach, thiol- and alkene-​modified oligonucleotide probes were covalently attached in microarray format, reaching immobilization densities around 6 pmol·cm-​2. The developed methodol. presents the advantages of spatially controlled probe anchoring (using a photomask)​, direct attachment without using cross-​linkers (one-​pot fashion)​, and short irradn. times (20 min)​. Using the described strategy, hybridization efficiencies up to 65​% with full complementary strands were reached. The approach was...

    A rapid strategy for the covalent immobilization of DNA onto silicon-​based materials using the UV-​initiated radical thiol-​ene reaction is presented in this study. Following this approach, thiol- and alkene-​modified oligonucleotide probes were covalently attached in microarray format, reaching immobilization densities around 6 pmol·cm-​2. The developed methodol. presents the advantages of spatially controlled probe anchoring (using a photomask)​, direct attachment without using cross-​linkers (one-​pot fashion)​, and short irradn. times (20 min)​. Using the described strategy, hybridization efficiencies up to 65​% with full complementary strands were reached. The approach was evaluated by scoring single-​base pair mismatches with discrimination ratios around 15. Moreover, the efficacy of the proposed DNA detection scheme is further demonstrated through the assay on a genomic target of bacterial Escherichia coli.

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    Volume 25
    Issue 3
    Pages 618-627

     

    DOI: 10.1021/bc500033d
    ISSN: 1043-1802
  • Direct and label-​free monitoring oligonucleotide immobilization, non-​specific binding and DNA biorecognition

    Lopez-Paz, Jose Luis; Gonzalez-Martinez, Miguel Angel; Escorihuela, Jorge; Banuls, Maria-Jose; Puchades, Rosa; Maquieira, Angel

    (2014). Article

    Sensors and Actuators, B: Chemical.

    DNA binding chem. on silicon surface has been investigated. Aminated oligonucleotide probes were immobilized on the chip surface by chem. silanization and further covalent attachment. The chemistries employed were the classical 3-​aminopropyltriethoxysilane​/glutaraldehyde and, for comparison purposes, the novel 3-​isocyanatepropyltriethoxysilane, that allows the direct attachment of the aminated probe. Alternatively, a thiolated oligonucleotide was also photochem. immobilized by means of a thioether linkage. The expts. were followed label-​free by Dual Polarization Interferometry. All chem. and photochem. methods gave rise to a probe d. immobilization in the order of 1.0-​2.5 × 1010...

    DNA binding chem. on silicon surface has been investigated. Aminated oligonucleotide probes were immobilized on the chip surface by chem. silanization and further covalent attachment. The chemistries employed were the classical 3-​aminopropyltriethoxysilane​/glutaraldehyde and, for comparison purposes, the novel 3-​isocyanatepropyltriethoxysilane, that allows the direct attachment of the aminated probe. Alternatively, a thiolated oligonucleotide was also photochem. immobilized by means of a thioether linkage. The expts. were followed label-​free by Dual Polarization Interferometry. All chem. and photochem. methods gave rise to a probe d. immobilization in the order of 1.0-​2.5 × 1010 mols.​/mm2, similar to the values reported for other chemistries. The obtained data suggest that DNA strands are anchored in a different conformation depending on the immobilization method employed. In order to avoid non-​specific binding of target mols., ethanolamine and inert proteins were assayed, and successful results were obtained when using small size proteins such as gelatine. Hybridization efficiency was around 20​% for aminosilane-​based immobilized probes, and more than 4-​fold this value when the other immobilization methods were employed. The ability for recognition complementary DNA strands discriminating non-​complementary ones was applied for species identification in mixts.

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    Volume 192
    Pages 221-228
     

    DOI: 10.1016/j.snb.2013.10.110
    ISSN: 0925-4005
  • Array-on-a-disk? How Blu-ray technology can be applied to molecular diagnostics

    Morais, Sergi; Tortajada-Genaro, Luis; Maquieira, Angel

    (2014). Article

    Expert Review of Molecular Diagnostics. No.7

    A review. This editorial comments on the balance and perspectives of compact disk technol. applied to mol. diagnostics. The development of sensitive, rapid and multiplex assays using Blu-​ray technol. for the detn. of biomarkers, drug allergens, pathogens and detection of infections would have a direct impact on diagnostics. Effective tests for use in clin., environmental and food applications require versatile and low-​cost platforms as well as cost-​effective detectors. Blu-​ray technol. accomplishes those requirements and advances on the concept of high d. arrays for massive screening to achieve the demands of point of care or in situ anal.


    Volume 14
    Issue 7
    Pages 773-775

     

    DOI: 10.1586/14737159.2014.929945
    ISSN: 1473-7159
  • Aluminum Nanohole Arrays Fabricated on Polycarbonate for Compact Disc-​Based Label-​Free Optical Biosensing

    Barrios, C. A.; Canalejas-Tejero, V.; Herranz, S.; Moreno-Bondi, M. C.; Avella-Oliver, M.; Puchades, R.; Maquieira, A.

    (2014). Article

    Plasmonics. No.3

    Al nanohole array plasmonic biosensors have been fabricated on polycarbonate (PC) substrates from conventional compact disks (CD)​. Std. micro and nanofabrication processes have been used and optimized to be PC compatible. The viability of this CD-​based plasmonic platform for label-​free optical biosensing has been demonstrated through a competitive bioassay for biotin anal. using biotin-​functionalized dextran-​lipase conjugates immobilized on the transducer surface.

    Volume 9
    Issue 3
    Pages 645-649

    DOI: 10.1007/s11468-014-9676-5
    ISSN: 1557-1955
  • Site-specific immobilization of DNA on silicon surfaces by using the thiolyne reaction

    Escorihuela Fuentes, Jorge; Bañuls Polo, Mª José; Puchades Pla, Rosa; Maquieira Catalá, Ángel

    (2014). Article

    Journal of materials chemistry B. No.48

    Número: 48  Volumen: 2  Páginas: 8510-8517