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Type: Techniques
Description

Western Blot is a widely used analytical technique for the study of proteins. This method allows the detection of a single protein within a biological sample. The specificity of Western Blot is achieved by using an antibody that recognises and binds to a single epitope of the protein of interest. The Western Blotting technique can estimate the size of a protein, confirm the presence of post-translational modifications such as phosphorylation, and be used to quantitatively compare protein levels between samples.