Fully equipped laboratory for sample preparation, acquisition, separation and analysis by flow cytometry.
The cytometry laboratory consists of several rooms: the cytometry room, where the analyser flow cytometers and auxiliary equipment are located, the separation room, where the cell separator and the flow cabinet are located, and the analysis room, where it is possible to analyse the data obtained in the equipment with specific software for this purpose.
Resources integrated in the cytometry laboratory:
|
EQUIPMENT |
BRAND AND MODEL |
NUMBER |
INVENTORY CODE |
DESCRIPTION |
|
LSRFORTESSA |
BECKTON DIKINSON LSR-FORTESSA SORP (SPECIAL ORDER RESERCH PRODUCT) |
1 |
72-179665 |
Flow cytometer analyser with 5 lasers (375nm, 405nm, 488nm, 561nm and 640nm). Detection of 20 parameters (18 fluorescence and 2 light scattering). |
|
FACSVerse |
BECKTON DIKINSON FACSVERSE 3L 8C |
1 |
72-179665 |
Flow cytometer analyser with 3 lasers (405nm, 488nm and 640nm). Detection of 10 parameters (8 fluorescence and 2 light scattering). HTS system for automated sample acquisition with capacity for 40 tubes. |
|
FACSCanto |
BECKTON DIKINSON FACSCANTO I |
1 |
72-162596 |
Flow cytometer analyser with 2 lasers (488nm and 640nm). Detection of 8 parameters (6 fluorescence and 2 light scattering). |
|
CELL SEPARATOR FacsAria Fussion |
BECKTON DIKINSON FACSAria Fusion |
1 |
72-206870 |
Flow cytometer Separator with 6 lasers (355nm, 405nm, 455nm, 488nm, 561nm and 640nm). Detection of 20 parameters (18 fluorescence and 2 light scattering). Allows separation of up to 4 subpopulations simultaneously in 1.5ml, 2ml and 5ml tubes, and up to 2 subpopulations in 15ml tubes.
|
|
SOFTWARE |
ENTERPRISE |
NUMBER |
INVENTORY CODE |
DESCRIPTION |
|
FLOWJO |
CELEZA GMBH |
1 |
23-171140 |
Software for analysing flow cytometry data. |
|
MODFIT |
BECKTON DIKINSON |
1 |
--------------- |
Software for cell cycle analysis. |
Además, el laboratorio dispone del siguiente equipamiento auxiliar:
|
EQUIPMENT |
BRAND AND MODEL |
NUMBER |
INVENTARY CODE |
DESCRIPTION |
|
BIOLOGICAL SAFETY CABINET |
TELSTAR BIO-II ADVANCE 3 |
1 |
72-210580 |
Type IIA biosafety cabinet for sample preparation. |
|
CENTRIFUGE |
EPPENDORF 5702 |
1 |
72-217839 |
Centrifuge for 5, 15 and 50 mL tubes with a maximum speed of 5,500 rpm. |
|
THERMBLOCKE |
FISHERBRAND |
1 |
72-214958 |
Digital dry bath for 1.5ml tubes |
|
FRIDGE AND REFRIGERATOR |
SANYO MPR 414F |
1 |
72-163852 |
COMBI with 340L refrigerator and 82L freezer for reagent storage. |
|
TUBES SHAKER |
HEIDOLPH Reax-Top |
1 |
72-143423 |
Vortex for sample and reagent agitation. |
|
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Analysis and separation of different cell types (animal, plant, yeast, bacteria, algae) by flow cytometry for subsequent cell culture or other studies (microscopy, proteomics, genomics, etc.).
Detection of cell subpopulations (immunophenotyping).
Apoptosis, viability and cell cycle assays.
Functional assays (metabolism, oxidation, proliferation, intracellular calcium, pH, etc.).
Studies at subcellular level (e.g. mitochondria).
Studies with prokaryotic cells, yeast and algae.
Absolute quantifications.
Multiplex assays (beads, arrays).
Advice to potential users on the applicability of this technique to their research.
ISO 9001:2015 Certification
Annual validation of the biosafety cabinets by an accredited external company.
Annual review of the separator by an external company
To be an authorised user of the Flow Cytometry Service. To this end, you must inform about the project, the collaborators who will be working on it, the use of biological agents and be familiar with the working rules. To become an independent user, you must receive adequate training from the technicians of the section.
- Norte Pinto, Sandra Cristina
- PIT-Investigacio Escala Tecnica Mitjana
