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Cell Culture laboratory

Cell Culture laboratory
Cell Culture laboratory
Type: Laboratory

Cell Culture laboratory of Biological Containment Level 2 (BCL2). It has several rooms: Cell Lines Room, Primary Cultures Room, Hypoxia Room and Viral Vectors Room (BCL2+), with 10 biosafety cabinets, 6 incubators and all the necessary equipment to work with eukaryotic cell cultures.

Prepared and controlled by technicians so the user just focuses on his experiment.

Description

The facilities of the cell culture laboratory consist of several rooms:

  • Cell Lines Room: with 6 laminar flow cabinets, 4 incubators, 2 cell counters and the necessary material to work on cell biology.
  • Primary Cultures Room: with 2 cabinets, an incubator, a cell counter and little equipment necessary for culture work.
  • Hypoxia Room: hypoxia station with 4 incubators with independent oxygen sensors.
  • BCL2+ Room: room for working with viral vectors or biological agents that require greater safety.

The laboratory is designed to work under level 2 biosafety conditions. It has acces control, entry locks, PVC scotia walls, HEPA air inlet filter and furniture on wheels.

Supply of basic culture consumables, media and supplements. More specific upon user request.

The facilities and their equipment allow working under aseptic conditions and monitoring cell growth. They also allow to carry out basic cell biology experiments.

The rooms also have COdetectors installed (and also Oin the hypoxia room) to guarantee the users safety.

Resources integrated in the Cell Culture laboratory:

EQUIPMENT

BRAND AND MODEL

NUMBER

INVENTORY CODE

DESCRIPTION

BIOLOGICAL SAFETY CABINET

TELSTAR

3 BIO-II A

7 BIO-II ADVANCE 4

10

72-210580

72-210580

72-111347

Rest, pending

Type IIA biosafety cabinet. It allows to protect the sample, the operator and the environment. 70% of air recirculation. 30% of air outdoor exit. It has EPA filters and germicide UV light for its disinfection.

CO2 INCUBATOR

THERMO FORMA FISHER

3 Steri-Cycle 371

2 Steri-CyCle i160

1 Cell Locker

6

72-111713

72-140794

72-111713

Rest, pending

Incubators for the growth and maintenance of cell cultures under optimal conditions of temperature, humidity and CO2 for cell growth. The Cell Locker also has separate compartments to minimise cross-contamination.

REFRIGERATED INCUBATOR

BINDER

KT115

1

72-219649

Incubator for the growth and culture of insect cells, or in need of lower temperatures.

HYPOXIA STATION

BIOSPHERIX

X VIVO

1

72-187465

Closed modular hypoxia station allowing to work with 4 independent hypoxia/hyperoxia conditions.

NITROGEN GENERATOR

PARKER

HPN2-12500C-E

1

Pending

Equipment connected to the hypoxia station to ensure N2 supply.

CELL COUNTERS

COUNTESSTM II AMQAX100

4

72-205663

Automatic cell counter allowing to distinguish between viable and non-viable cells.

INVERTED MICROSCOPE

NIKON Eclipse

TS-100

ZEISS Axio Vert

3

72-177948

72-181830

Pending

Inverted microscopes for monitoring cell growth and density. The Zeiss microscope has an integrated camera and 4x, 10x, 20x and 40x objectives.

FLUORESCENCE MICROSCOPE

NIKON

Eclipse TE-2000S

1

72-111342

Fluorescence microscope with an integrated camera. 10x, 20x and 40x objectives.

CELL IMAGING AUTOMATIC SYSTEM

NanoEnTeck,

JULI LG04 (GFP)

1

72-217785

Green fluorescence microscope able to programme image acquisition over time.

CENTRIFUGES

EPPENDORF 5810R

EPPENDORF 5702R and 5702

4

72-138248

Rest pending

Centrifuges specially designed for cell culture laboratories. Maximum speed of 5,500 rpm.

MICROULTRACENTRIFUGE

HIMAC

CS150FNX

1

72-217783

Floor standing ultracentrifuge with 3 rotors: S55A2, S50A and swing-out rotor S52ST.

MICROPLATE READER

THERMO FORMA FISHER

Multiscan Ex

1

72-107232

Absorbance plate reader with filters at: 340, 405, 414, 450, 570. 595. 620 and 650 nm.

THERMOSTATIC BATHS

RAYPA BAE-2

5l C/FP

7

72-100091

72-131621

72-217733

72-217733

72-131621

72-122440

72-76415

Homogeneous temperature of 37°C for tempering means, reagents, samples, etc.

SUCTION SYSTEM

VACUSAFE

Comfort Plus

11

5 72-155095

3 72-218098

3 72-194691

Suction, collection and subsequent disposal of biological liquid waste system.

DOUBLE DOOR AUTOCLAVE

UNISTERI HP

559-2ED

1

72-205130

Autoclave for sterilisation of waste and contaminated materials.

SINGLE DOOR AUTOCLAVE

UNISTERI HP

336-1ED

1

72-205128

Autoclave for sterilisation of materials and reusable consumables.

4º C FRIDGES

INFRICO

LTF65GD

5

72-217557

Maintenance of users and section media, supplements and reagents.

-20ºC FREEZERS

INFRICO

LTF65GD

3

72-217558

Maintenance of users and section supplements and reagents.

-80ºC ULTRAFREEZERS

SANYO 333L VIP SEG. FRED

1

72-183391

-80°C freezer for freezing cell lines prior to the transfer to cryogenic tanks.

-80ºC ULTRAFREEZER

INFRICO

1

Pending

-80ºC underbench freezer for virus room.

PRECISION SCALE

KER 572-33

1

72-99802

Weighing range [Max]: 1,600 g, Readability [d]: 0.01 g, Reproducibility: 0.01 g, Linearity: 0.03 g, Weighing pan: Ø 150 mm (Stainless steel).

LUMINOMETER

LONZA

Luceta simple sample

1

72-177956

Equipment for mycoplasma analysis in culture medium.

tube SHAKER

HEIDOLPH

Reax-Top

6

72-131619

72-131620

Vortex tube shaker.

TISSUE DISSOCIATOR

MILTENYI BIOTEC gentleMACS™

1

72-194689

Tissue dissociator that provides greater efficacy than conventional methods. Reduces the amount of cellular waste and cell death. With specific protocols for certain cell types.

IMMUNOMAGNETIC SEPARATOR

MILTENYI BIOTEC MINI & MIDIMACS

1

72-194684

Immunomagnetic cell separator using columns and antibodies attached to magnetic beads.

SEMIAUTOMATIC outdoor DISTRIBUTION PLANT

of gases with:

4 N2 cylinders,

4 CO2 cylinders and

4 O2 cylinders

AIR LIQUIDE

1

Pending

Distribution plant that guarantees the continuous supply of gases to the room.

It is simultaneously connected to the nitrogen generator so that, in the event of generator breakdown, it ensures the N2 supply to the hypoxia station.

Gas detection CONTROL UNIT

OLDHAM MX32

with CO2 and O2 detectors

5

72-216080

Control unit for O2 and CO2 levels in the hypoxia room and for CO2 level in the rest, with acoustic and light alarm.

  • Heathrow Scientific/Fisher Microcentrifuge.
  • Complete set of EPPENDORF Research plus micropipettes for each cabinet.
  • Controlled freezing devices.
  • Racks.
  • PPE.
  • Fungible and products for cleaning and disinfection.
Practical implementation

It allows to work with level 2 biological agents: eukaryotic cell culture.

  • Working with established cell lines.
  • Carrying out primary cultures.
  • Carrying out co-cultures.
  • Generating and working with viral vectors.
  • Experimenting with GMOs.
  • Working with cells subjected to hypoxia and hyperoxia.
  • Developing cell biology techniques.
  • Detecting mycoplasma contaminations.
  • Freezing, thawing and amplifying cell line stocks.
  • Testing at researcher request.
Quality certifications

ISO 9001:2015 certification

Annual validation of the Biosafety cabinets by an accredited external company

Annual review of incubators by an external company

Six-monthly review of the Hypoxia Station

Terms of use

To be an authorised user in the Cell Culture Service. To this end, you must inform about the project, the collaborators who will be working on it, the use of biological agents, be familiar with the working rules and take a guided tour by the section’s technicians.

Access to the facilities is allowed wearing a lab coat, overshoes and a mask provided by this section. They are for exclusive use in the laboratory.