Search result71 results
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Delivery of liquorice extract by liposomes and hyalurosomes to protect the skin against oxidative stress injuries
Castangia I, Caddeo C, Manca ML, Casu L, Latorre AC, Díez-Sales O, Ruiz-Saurí A, Bacchetta G, Fadda AM, Manconi M.
(2015). ArticleCarbohydr Polym., vol. 134, pp. 657-63
DOI: 10.1016/j.carbpol.2015.08.037 -
Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring
Manca ML, Castangia I, Zaru M, Nácher A, Valenti D, Fernàndez-Busquets X, Fadda AM, Manconi M.
(2015). ArticleBiomaterials, vol. 71, pp. 100-109
DOI: 10.1016/j.biomaterials.2015.08.034 -
Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)
Tortajada-Genaro, Luis A.; Santiago-Felipe, Sara; Amasia, Mary; Russom, Aman; Maquieira, Angel
(2015). ArticleRSC Advances. No.38
A new advancement in massive DNA-based screening in limited-resource settings is demonstrated through the incorporation of easy-to-fabricate microfluidic chambers on digital versatile disks (DVDs) to perform isothermal recombinase polymerase amplification (RPA) in a microarray format. Std. un-modified DVD disks and com. drives are used for the low-cost detection method. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-negatives and false-positives. The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of...
Read more [Read more] DOI: 10.1039/C5RA02778KISSN: 2046-2069 -
La otra música de los discos compactos. Biosensores ópticos derivados de tecnologías de electrónica de consumo
Maquieira Catalá, Ángel; Puchades Pla, Rosa; Tortajada Genaro, Luis Antonio; Bañuls Polo, Mª José; Morais Ezquerro, Sergi Beñat
(2015). ArticleBoletín de la Sociedad Española de Química Analítica. No.49
Páginas 11-14
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Nanogold bioconjugates for direct and sensitive multiplexed immunosensing
P. Dobosz, S. Morais, R. Puchades, A. Maquieira
(2015). ArticleBiosensors and Bioelectronics.
The use of nanogold bioconjugates for direct detection of the antibody–antigen immunoreaction is addressed. The integration of gold nanoparticles tracers as signal generators in microarray immunosensing and compact disc detection technique show important advantages to reach sensitive, selective, high throughput, reliable and cost-effective assays. For that, a thorough study of the performances of the size of spherical nanogold particles and coating density was developed. The size of the nanoparticle determines the optimal antibody dilution, being the smaller particles the best performing ones. Enhancement effect of lower size is also studied. The gold labeling method do not affects the...
Read more [Read more] DOI: 10.1016/j.bios.2015.03.007ISSN: 0956-5663 -
Massive Immuno Multiresidue Screening of Water Pollutants
Dobosz, Paulina; Morais, Sergi; Bonet, Emilio; Puchades, Rosa; Maquieira, Angel
(2015). ArticleAnalytical Chemistry. No.19
An immuno multiresidue screening assay in microarray format for the detn. of complex chem. mixts. at the μg/L level, using antibody-functionalized Au nanoparticles, is presented. The anal. method relies on the use of a cocktail of nanogold-labeled specific antibodies, acting as recognition and detection species. The concept of multireside screening is proved by developing a multiplex assay on a compact disk support for the detn. of 2-(2,4,5-trichlorophenoxy)propionic acid, 3-phenoxybenozic acid, 4-nitrophenol, alachlor, atrazine, azoxystrobin, chlorpyrifos, diazinon, diuron, endosulfan, fenthion, forchlorfenuron, imidacloprid, malathion, pentachlorophenol, pyraclostrobin,...
Read moreVolume 87 Issue 19 Pages 9817-9824
DOI: 10.1021/acs.analchem.5b02354ISSN: 0003-2700 -
Isothermal DNA amplification strategies for duplex microorganism detection
Santiago-Felipe, Sara; Tortajada-Genaro, Luis Antonio; Morais, Sergi; Puchades, Rosa; Maquieira, Angel
(2015). ArticleA valid soln. for micro-anal. systems is the selection of a compatible amplification reaction with a simple, highly-integrated efficient design that allows the detection of multiple genomic targets. Two approaches under isothermal conditions are presented: recombinase polymerase amplification (RPA) and multiple displacement amplification (MDA). Both methods were applied to a duplex assay specific for Salmonella spp. and Cronobacter spp., with excellent amplification yields (0.2-8.6 · 108 fold). The proposed approaches were successfully compared to conventional PCR and tested for the milk sample anal. as a microarray format on a compact disk (support and driver). Satisfactory...
Read moreVolume 174
DOI: 10.1016/j.foodchem.2014.11.080ISSN: 0308-8146
Pages 509-515 -
Dual-Polarization Interferometry: A Novel Technique To Light up the nanomolecular World
Escorihuela, Jorge; Gonzalez-Martinez, Miguel Angel; Lopez-Paz, Jose Luis; Puchades, Rosa; Maquieira, Angel; Gimenez-Romero, David
(2015). ArticleChemical Reviews. No.1
A review. The topics include: general principles, measurement, comparison and combination with other methods, bioreceptor immobilization and applications.
[Read more] DOI: 10.1021/cr5002063ISSN: 0009-2665 -
Synthesis and evaluation of the chromo-fluorogenic recognition ability of imidazoquinoline derivatives toward ions
Marín-Hernández, C., Santos-Figueroa, L. E., El Sayed, S., Pardo, T., Raposo, M. M. M., Batista, R. M., Costa, P.G. S., Martínez‐Mañez, R., Sancenón, F.
(2015). ArticleDyes and Pigments, vol. 122, p. 50-58.
DOI: 10.1016/j.dyepig.2015.06.011
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Antifungal effect of essential oil components against Aspergillus niger when loaded into silica mesoporous supports
Bernardos, A., Marina, T., Žáček, P., Pérez‐Esteve, É., Martínez‐Mañez, R., Lhotka, M., Kouřimská, L., Pulkrábek, Josef., Klouček, P.
(2015). ArticleJournal of the Science of Food and Agriculture, vol. 95, no 14, p. 2824-2831.
DOI: 10.1002/jsfa.7022