A

IN VITRO TECHNIQUES
1. Biochemical
1.1. Determination by ELISA of inflammatory mediators; pruritogenic ...
1.2. Determination of inflammatory markers by WB
1.3. Determination of active compound by HPLC (fluorescence detector, ultraviolet-visible, and refractive index)
1.4. Determination of intracellular Ca fluorescence microscopy in real time.
1.5. Flow cytometry. Study in whole blood and cell subpopulations. Apoptosis / necrosis, characterization of cell populations, expression of surface molecules ...

2. Molecular
2.1. Automatic extraction of RNA and genomic DNA
2.2. EMSA.
2. 3. RT-PCR
2.4. Cloning, sequencing, chips, expression, polymorphism, transcriptómic chips, 2.5. Northern-blotting, Southern-blotting, FIS. Luciferase transfection
 
3. Histological and immunohistochemical
 
4. Cell
4.1. Procurement of primary human skin cell lines:
4.2. Cultivation of cell lines
4.3. Cultivation of differentiated epithelia
4.4. As epithelial resitencia (TEER)
4.4. Cell transfection
 
5. "Flow Chamber"
5.1. Study of leukocyte-endothelium interactions on primary cultures of human endothelial cells (HUVECs, HUAECs)
5.2. Studies on whole blood or leukocyte subtypes isolated from individuals
healthy or with inflammatory disease
 
6. Microbiological

IN VIVO TECHNIQUES 
1.1. Animal models

1.2. Intravital microscopy: study of leukocyte-endothelium interaction. [1-4]
1.2.1. Rat mesentery: stimuly: LPS, cytokines, chemokines.
1.2.2. Mouse cremaster: stimuly: LPS, cytokines, chemokines, tobacco smoke extract
and OVA antigenic "challenge".

1.3. Molecular imaging techniques (Oncovision Albira)
1.3.1. micro-CT
1.3.2. micro-CT / PET
1.3.3. micro-CT / SPECT

 B

Our main objective is to evaluate the safety of cosmetic products through alternative methods validated as well as clinical studies in healthy volunteers
Our job is to ensure that cosmetic products are put on the market; with a guarantee of security of the same; and contributing, no doubt, to improve the level of product quality.
METHODS IN VITRO
Cosmetics lab conducts safety assessments of cosmetics as directed by the guidelines of the OECD and low standards of Good Laboratory Practice (Official Certificate of Compliance with GLP requirements of the Spanish legislation)
http://www.aemps.gob.es/industria/inspeccionBPL/listadoLab-BPL.htm
Certificate No: BPL 1.12 / 13C.VAL.
Ref: DGFPS / SGPTF / SOCVPF / SIIF

Experienced in the following techniques:

 BCOP test of eye irritation / corrosion
REGULATORY STANDARD OECD TG437
Opacitometer Kit BASF-OP3.0

Episkin test for skin corrosion test
REGULATORY STANDARD OECD TG 431

Episkin test for skin irritation test
REGULATORY STANDARD OECD TG 439

In vitro diffusion test to measure skin absorption in human skin: determination of the substance absorbed by HPLC

REGULATORY STANDARD OECD TG 428
System cells In vitro Franz & Tewameter VT 310
Test 3T3 NRU phototoxicity in vitro (photoirradiation)

REGULATORY STANDARD OECD TG 432
Solar Simulator UVACUBE400-coupled to sol500
 (UV-VIS-IR filters)

METHODS IN VIVO "trials"
We also evaluated the safety and efficacy of cosmetic products in healthy volunteers following the ethical and good clinical practices relevant premises.
-SECURITY IN VIVO:
• PATCH TEST / single application
• FOTOPATCH TEST
• USE TEST UNDER CLINICAL CONTROL
• OPEN TEST
 
-Efficacy IN VIVO:
• Moisturizing Effect
Instrumental assessment of the immediate or accumulative moisturizing efficacy. Measuring skin capacitance.
Corneometer®
• TENSOR / LIFTING EFFECT
Instrumental assessment of the tensioner / immediate lifting effect. Measuring the extensibility of the skin (skin deformation under partial vacuum) and skin elasticity (ability of the skin to return to its initial state)
Cutometer®
• firming effect: ELASTICITY skin tone
Instrumental assessment firming / effectiveness of elasticity skin tone effectively. Measuring the extensibility of the skin (skin deformation under partial vacuum) and skin elasticity (ability of the skin to return to its initial state)
Cutometer®
• WRINKLE EFFECTIVENESS
Instrumental assessment of the anti-wrinkle efficacy
Visioscan & Cutometer®
• EFFICIENCY ANTIEDAD
Instrumental assessment of the anti-aging effectiveness
Visioscan & Cutometer®
• EFFICIENCY REGENERANTE
Instrumental assessment of the regeneration efficiency. Evaluation of "turn-over" cell by comparing the kinetic curves of decreased skin color obtained by applying DHA (dihydroxyacetone) in an area of skin treated with the product and in a control zone by colorimetry
Mexameter® & Colorimeter®
• EFFECTIVE SLIMMING / REDUCING
Instrumental assessment of slimming efficacy and reductive. Determination of the body weight of the volunteers and SHF measurements of various body parts (thighs, waist, hips ...) using a tape measure.

• EFFECTIVE SELF-TANNING
Instrumental assessment of the self-tanning efficacy. Evaluation of skin color induced product by colorimetric measurements.
Mexameter® & Colorimeter®
• TAN ACCELERATOR EFFECT AND / OR MAINTENANCE Instrumental assessment of the tanning accelerating effect. Lavelocidad assessment of obtaining skin tanning by colorimetric measurements. And their maintenance
Mexameter® & Colorimeter®
• SEBO-REGULATING EFFECT
instrumental evaluation of regulatory effectiveness tallow
Sebumeter®
• EFFECT ON THE LEVEL OF EFFECT SEBO-MATE
Instrumental evaluation of the effect on the level of fat / matt effect. As the level of sebum.
Sebumeter®
• EFFECT comedolytic
comedolytic clinical evaluation of efficacy. Retentional count of acne lesions (comedones).
• bleaching effect
Instrumental assessment of the bleaching efficiency. Evaluation of the variation of skin color by colorimetric measurements on a panel of volunteers with a particular skin tone.
Mexameter® & Colorimeter®
• EFFECT ON DARK SPOTS DEPIGMENTING
Instrumental assessment of the depigmenting efficacy on dark spots. Mexameter® & Colorimeter®
• calming effect
soothing instrumental evaluation of efficacy after induction of sunburn. colorimetric measurements with erythematous skin reaction (non immune response) induced by UVB exposure and a small evolution in time after application of the product.
Solar simulator 16S-300 & Mexameter® & Colorimeter®
• Sun Protection Factor (SPF)
Evaluation of Sun Protection Factor according to the INTERNATIONAL SUN PROTECTION FACTOR TEST METHOD CTFA (SA) -COLIPA-JCIA-CTFA (USA) / May 2006. Clinical evaluation of erythematous skin response to ultraviolet radiation.
Solar Simulator 16S-300®
• Sun Protection Factor + Water resistance (FPS + WR)
Evaluation of Sun Protection Factor according to the INTERNATIONAL SUN PROTECTION FACTOR TEST METHOD CTFA (SA) -COLIPA-JCIA-CTFA (USA) / May 2006 and evaluation of water resistance according to COLIPA GUIDELINES FOR EVALUATING WATER PRODUCT SUN RESISTANCE-December 2005.
Solar Simulator 16S-300® & Equipment Immersion Spa pool