There are several galleries depending on the microorganism to be studied:

• API 20E allows identification of microorganisms belonging to enterobacteria and other Gram negative bacilli
• API 20NE allows identification of Gram negative bacilli not belonging to enterobacteria group such as Pseudomonas, Acinetobacter, Flavobacterium, Moraxella, Vibrio, Aeromonas and others
• API 20A allows identification of anaerobic bacteria
• API 50CH allows metabolic study of carbohydrates and identification of Bacillus (CHB), Lactobacillus (CHL) and Enterobacteriaceae (CHE).

Necessary material

Active culture of the strain (Petri dish or slant).

Methodology

Manufacturer's recommendations are followed to characterize bacterial and yeast strains using the API and bioMérieux ID 32 miniaturized systems.

The report provided by CECT contains the biochemical profile obtained and the result generated by comparing the profile with those in the bioMérieux database.

Note: as in any other identification system some isolates give inconclusive results, which appear in the report as low discrimination, unreliable identification, questionable or unacceptable profile.