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Lichens: Conservation Biology. Biodiversity and Biogeography

Conservation Biology. Lichen diversity indices, European Network of Environmental Quality. Designs in relation to the Forest Health Programme of the USDA Forest Service, USA. Applications to natural areas management and forestry handling.

In Biodiversity Heritage

Techniques that dominates:

  • Photobionts lichen insulation. Axenic cultures of symbiotic algae
  • Modulated fluorescence of chlorophyll, kinetics (fluorometer, actinic light)
  • Enzymatic ratings and lipid peroxidation
  • Photosynthesis performance. Xanthophyll pigments cycle analysis
  • Determination of ascorbate. Antioxidant enzymes
  • Mono-and two-dimensional electrophoresis of proteins, Western blot, "Northern blot", isozyme patterns and related techniques
  • Centrifuge of tubes or plates.
  • Microscopy and ultrastructures (MO, epifluorescence, TEM, and SCM LTSM, confocal), fluorochrome, staining and inclusions, antibodies.
  • Algae and microorganisms incubation chambers. Refrigerated culture ovens
  • Laminar flow chambers and Autoclaves
  • Spectrophotometers. Spectrofluorometers
  • Chromatography: TLC. HPLC, CZE
  • Fumigation systems with pollutant gases (ozone, sulphur dioxide and nitrogen oxides)
  • OTCs (Open Top Chambers)
  • Exhaust Hoods
  • Chamber at 4 °C and freezers at -20 ° C to -80 ° C.

Cork Quality Bioindication ( Lichens and character of trees )

Trees external characters. Predictive models:

  • Lichens.
  • Climate physical and chemical parameters of Reproduction cork anatomy and density (physical properties). Biophotonics.
  • Cork: radial growth and density.
  • Patent. Registration No. : 2068156 A1. Authors: Barreno, E. & Fos, S.
  • Title: Process for the preparation of thin sections from Quercus suber cork l . Biophotonics .
  • Applications to the characterization of the Valencian corks.

Air Pollution Bioindication

Lichens. Trees. Mycorrhizae. Agricultural crops.

Field conditions:

  • Networks of Biological Control and monitoring of air quality in large areas (passive monitoring). Valencia , Canary Islands, León, Madrid, Galicia, Italy, mountains of San Bernardino and Santa Barbara (California).

Laboratory conditions:

  • Leaching of inorganic ions. In collaboration with Guillermo Ramis -Ramos, Analytical Chemistry, UVEG. Six published studies (Needles, Lichens)
  • Fumigation with pollutants gases: O3, SO2 and NOx, in closed systems. (Lichens, Bryophytes).
  • Activity of antioxidant enzymes. Lichens and crop plants.
  • Identification of genes NADH: finding in lichen and algae crop plants. No species of conifers (pines). Two manuscripts submitted to international journals and several works being drafted.

Mixed field and laboratory conditions:

  • OTC (open top chambers) with weather station (Exp Est Carcaixent, Department of Agriculture). Agricultural crops (lichen and pine) Results: We have tested commercial and sensitive varieties of Valencian crops. The physiological and morphological response is varied.