The Genomics Unit is extensively experienced in the field of sequencing and provides access to the most technologically advanced mass analysis platforms.

Sequencing technologies available in the unit include:

• Illumina (MiSeq, NextSeq 550)
• PacBio (Sequel System)

Having these technologies, short sequences can be obtained with second generation technologies (Illumina) and long sequences from single molecule (PacBio), thus providing researchers with the chance to combine sequencing strategies to tackle different genomic and transcriptomic approaches.

The unit offers consultancy on experiments design and on the choice of the most appropriate technology for it. In addition, the laboratory processes both DNA and RNA samples: purification, quality control, preparation of libraries and sequencing on the chosen platform.

All sequencing data obtained are subjected to a preliminary analysis to ensure the data quality before being delivered to the researcher. The preliminary analysis consists of filtering out low-quality sequences and removing sequencing adapters. Once it has been carried out, the data are available for download on a secure server and will be stored in our systems during a maximum period of 3 months.

We also offer more advanced biocomputing analyses such as: genome and transcriptome assembly, annotation, variant detection, differential expression analysis, determination of taxonomic variability in microbial communities (16S, ITS, 18S), shot-gun metagenomics, single cell analysis and phylogenetic analysis among others.

To discuss the objectives and requirements of each project, please contact the staff of the unit (genoscsie@uv.es)

Applications:

• De Novo Sequencing of bacterial, viral, plant, animal and human genomes.
• Resequencing of genomes: detection of SNPs, CNVs, etc.
• Amplicon sequencing (16S rRNA, 18S rRNA, ITS, etc).
• Metagenome and metatranscriptome sequencing to assess bacterial, archaeal or eukaryotic diversity and detect abundance in environmental samples.
• Duplex-seq: sequencing for low-frequency mutation detection.
• RNA-seq: transcriptome analysis, differential expression studies.
• Iso-Seq: sequencing of complete transcripts and detection of splicing variants.
• miRNA sequencing.
• Epigenetics: ChIP-seq, Methyl-Seq, MeDIP-seq, etc.
• Single cell genomic and transcriptomic analysis.