Resultados de la búsqueda58 resultados
-
Real-timeloop-mediated isothermal DNA amplification in compact disc micro-reactors
Sara Santiago-Felipe, Luis A. Tortajada-Genaro, Javier Carrascosa, Rosa Puchades, Ángel Maquieira
(2016). ArticleAn integrated device composed of micro-reactors embedded onto compact discs is proposed for real-time targeted DNA determination. The method principle is based on in-disc loop-mediated isothermal amplification (iD-LAMP) and quantitative optical read-out by a disc drive. In the presence of a target, the turbidimetric or colorimetric properties of reaction solution change, and the transmitted intensity of the disc drive laser modifies according to reaction yield. Monitoring real-time curves allowed the quantitative determination of DNA template amounts. The best amplification/detection results were obtained with micro-reactors (2 mm diameter and 1.1 mm in depth) drilled on a digital video...
An integrated device composed of micro-reactors embedded onto compact discs is proposed for real-time targeted DNA determination. The method principle is based on in-disc loop-mediated isothermal amplification (iD-LAMP) and quantitative optical read-out by a disc drive. In the presence of a target, the turbidimetric or colorimetric properties of reaction solution change, and the transmitted intensity of the disc drive laser modifies according to reaction yield. Monitoring real-time curves allowed the quantitative determination of DNA template amounts. The best amplification/detection results were obtained with micro-reactors (2 mm diameter and 1.1 mm in depth) drilled on a digital video disc (DVD) and detection based on the colorimetric mode. As proof-of-concept, the assay was applied to detect pathogenic bacteria Salmonella spp. and to identify bovine meat in food samples. Ninety-six samples were simultaneously analysed in 15 min, with high selectivity and sensitivity (5 CFU/mL and 10 µg/g for bacteria and meat, respectively). The in-disc results were comparable to those obtained by conventional LAMP or qPCR approaches. The developed device allows low sample and reagent consumption (3 µL of reaction), portability, ease-of-use, and rapid low-cost high-throughput analyses.
Leer más OcultarVolume 79, Pages 300–306
DOI: 10.1016/j.bios.2015.12.045 -
Targeting Innate Immunity with dsRNA-Conjugated Mesoporous Silica Nanoparticles Promotes Antitumor Effects on Breast Cancer Cells
Ultimo, A., Giménez, C., Bartovsky, P., Aznar, E., Sancenón, F., Marcos, M. D., Amorós, P., Bernardo, A. R., Martinez-Manez, R., Jiménez‐Lara, A. M., Murguía, J. R.
(2016). ArticleChemistry–A European Journal, vol. 22, no 5, p. 1582-1586.
DOI: 10.1002/chem.201504629
-
Thrombin-Responsive Gated Silica Mesoporous Nanoparticles As Coagulation Regulators
Bhat, R., Ribes, A., Mas, N., Aznar, E., Sancenón, F., Marcos, M. D., Murguia, J. R., Venkataraman, A., Martínez-Máñez, R.
(2016). ArticleLangmuir, vol. 32, no 5, p. 1195-1200.
DOI: 10.1021/acs.langmuir.5b04038
-
Multi-output Model with Box-Jenkins Operators of Quadratic Indices for Prediction of Malaria and Cancer Inhibitors Targeting Ubiquitin- Proteasome Pathway (UPP) Proteins.
Casañola-Martin GM, Le-Thi-Thu H, Pérez-Giménez F, Marrero-Ponce Y, Merino-Sanjuán M, Abad C, González-Díaz H.
(2016). ArticleCurr. Protein Pept. Sci., vol. 17, pp. 220-7
-
Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc
Santiago-Felipe, Sara; Tortajada-Genaro, Luis Antonio; Puchades, Rosa; Maquieira, Ángel.
(2016). ArticleMicrochimica Acta, vol. 3 (183), p. 1195 - 1202.
DOI: 10.1007/s00604-016-1745-3 -
Gated Materials for On-Command Release of Guest Molecules
Aznar, E., Oroval, M., Pascual, L., Murguía, J. R., Martinez-Manez, R., Sancenon, F.
(2016). ArticleChemical reviews, vol. 116, no 2, p. 561-718.
DOI: 10.1021/acs.chemrev.5b00456
-
Sensing and discrimination of cyanide and hydrogen sulfide using an 8-alkenylBODIPY derivative
E. Alvarado-Martínez; A. M. Costero; S. Gil; E. Peña-Cabrera
(2016). ArticleRSC Advances, vol. 6, pp. 179-182
-
Cross-linked chitosan/liposome hybrid system for the intestinal delivery of quercetin.
Caddeo C, Díez-Sales O, Pons R, Carbone C, Ennas G, Puglisi G, Fadda AM, Manconi M.
(2016). ArticleJ. Colloid Interface Sci., vol. 461, pp. 69-78
DOI: 10.1016/j.jcis.2015.09.013