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  • Delivery of liquorice extract by liposomes and hyalurosomes to protect the skin against oxidative stress injuries

    Castangia I, Caddeo C, Manca ML, Casu L, Latorre AC, Díez-Sales O, Ruiz-Saurí A, Bacchetta G, Fadda AM, Manconi M.

    (2015). Article

    Carbohydr Polym., vol. 134, pp. 657-63

    DOI: 10.1016/j.carbpol.2015.08.037
  • Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring

    Manca ML, Castangia I, Zaru M, Nácher A, Valenti D, Fernàndez-Busquets X, Fadda AM, Manconi M.

    (2015). Article

    Biomaterials, vol. 71, pp. 100-109

    DOI: 10.1016/j.biomaterials.2015.08.034
  • Isothermal solid-​phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)

    Tortajada-Genaro, Luis A.; Santiago-Felipe, Sara; Amasia, Mary; Russom, Aman; Maquieira, Angel

    (2015). Article

    RSC Advances. Num.38

    A new advancement in massive DNA-​based screening in limited-​resource settings is demonstrated through the incorporation of easy-​to-​fabricate microfluidic chambers on digital versatile disks (DVDs) to perform isothermal recombinase polymerase amplification (RPA) in a microarray format. Std. un-​modified DVD disks and com. drives are used for the low-​cost detection method. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-​negatives and false-​positives. The solid-​phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of...

    A new advancement in massive DNA-​based screening in limited-​resource settings is demonstrated through the incorporation of easy-​to-​fabricate microfluidic chambers on digital versatile disks (DVDs) to perform isothermal recombinase polymerase amplification (RPA) in a microarray format. Std. un-​modified DVD disks and com. drives are used for the low-​cost detection method. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-​negatives and false-​positives. The solid-​phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solns. through the inlet and by controlling the flow-​movement by DVD drive centrifugation. The final disk with reaction products was inserted into a DVD player and microarray images were captured and automatically processed. This simple approach was applied for the screening of genetically modified organisms (GMOs) in food samples. The limit of detection was 7 μg g-​1, which is well below the EU regulation limit for GMOs in food products. Therefore, the only required materials for food safety monitoring were std. store-​bought DVDs, plastic chambers, tips, pipettes, an oven, and a std. DVD drive. The proposed strategy allows an integrated microarray system with low manipulation, reduced sample vol., and portability, which are beneficial for low-​resource settings.

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    Volume 5
    Issue 38
    Pages 29987-29995

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    DOI: 10.1039/C5RA02778K
    ISSN: 2046-2069
  • La otra música de los discos compactos. Biosensores ópticos derivados de tecnologías de electrónica de consumo

    Maquieira Catalá, Ángel; Puchades Pla, Rosa; Tortajada Genaro, Luis Antonio; Bañuls Polo, Mª José; Morais Ezquerro, Sergi Beñat

    (2015). Article

    Boletín de la Sociedad Española de Química Analítica. Num.49

    Páginas 11-14

  • Nanogold bioconjugates for direct and sensitive multiplexed immunosensing

    P. Dobosz, S. Morais, R. Puchades, A. Maquieira

    (2015). Article

    Biosensors and Bioelectronics.

    The use of nanogold bioconjugates for direct detection of the antibody–antigen immunoreaction is addressed. The integration of gold nanoparticles tracers as signal generators in microarray immunosensing and compact disc detection technique show important advantages to reach sensitive, selective, high throughput, reliable and cost-effective assays. For that, a thorough study of the performances of the size of spherical nanogold particles and coating density was developed. The size of the nanoparticle determines the optimal antibody dilution, being the smaller particles the best performing ones. Enhancement effect of lower size is also studied. The gold labeling method do not affects the...

    The use of nanogold bioconjugates for direct detection of the antibody–antigen immunoreaction is addressed. The integration of gold nanoparticles tracers as signal generators in microarray immunosensing and compact disc detection technique show important advantages to reach sensitive, selective, high throughput, reliable and cost-effective assays. For that, a thorough study of the performances of the size of spherical nanogold particles and coating density was developed. The size of the nanoparticle determines the optimal antibody dilution, being the smaller particles the best performing ones. Enhancement effect of lower size is also studied. The gold labeling method do not affects the recognition capability of the labeled proteins. As a proof of concept, the nanoconjugates were used for the simultaneous and direct determination of small molecules. Employing nanogold bioconjugates as recognition labels resulted in robust and reliable assays, reaching a sensitivity of 0.03 and 1.3 μg/L for sulfasalazine and atrazine, respectively. This shows that the use of nanogold bioconjugates for direct immunosensing is very competitive, achieving highly sensitive and reproducible assays (RSD<10%). This approach would simultaneously determine both small and large molecular size targets, in different formats, using the same detection mode what paves the way for many other applications in different scenarios.

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    Volume 69,  Pages 294–300

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    DOI: 10.1016/j.bios.2015.03.007
    ISSN: 0956-5663
  • Massive Immuno Multiresidue Screening of Water Pollutants

    Dobosz, Paulina; Morais, Sergi; Bonet, Emilio; Puchades, Rosa; Maquieira, Angel

    (2015). Article

    Analytical Chemistry. Num.19

    An immuno multiresidue screening assay in microarray format for the detn. of complex chem. mixts. at the μg​/L level, using antibody-​functionalized Au nanoparticles, is presented. The anal. method relies on the use of a cocktail of nanogold-​labeled specific antibodies, acting as recognition and detection species. The concept of multireside screening is proved by developing a multiplex assay on a compact disk support for the detn. of 2-​(2,​4,​5-​trichlorophenoxy)​propionic acid, 3-​phenoxybenozic acid, 4-​nitrophenol, alachlor, atrazine, azoxystrobin, chlorpyrifos, diazinon, diuron, endosulfan, fenthion, forchlorfenuron, imidacloprid, malathion, pentachlorophenol, pyraclostrobin,...

    An immuno multiresidue screening assay in microarray format for the detn. of complex chem. mixts. at the μg​/L level, using antibody-​functionalized Au nanoparticles, is presented. The anal. method relies on the use of a cocktail of nanogold-​labeled specific antibodies, acting as recognition and detection species. The concept of multireside screening is proved by developing a multiplex assay on a compact disk support for the detn. of 2-​(2,​4,​5-​trichlorophenoxy)​propionic acid, 3-​phenoxybenozic acid, 4-​nitrophenol, alachlor, atrazine, azoxystrobin, chlorpyrifos, diazinon, diuron, endosulfan, fenthion, forchlorfenuron, imidacloprid, malathion, pentachlorophenol, pyraclostrobin, sulfasalazine, and triclosan, achieving detection limits of 0.07, 0.24, 10.9, 0.21, 0.14, 0.11, 0.11, 102, 0.36, 1.8, 1.7, 0.06, 0.08, 5.8, 1.0, 0.39, 0.003, and 12 μg​/L, resp. Due to the selectivity of the antibody-​functionalized nanoparticles, the developed screening methodol. allows the simultaneous detn. of mixts. of water pollutants in a 10-​plex configuration. The anal. performances were compared with those of ref. chromatog. methods by the anal. of spiked water samples, the sensitivity and recovery results being in good agreement. The presented screening approach directly quantifies the concn. of complex chem. mixts. without sample treatment or preconcn. steps in a total time of 35 min.

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    Volume 87 Issue 19 Pages 9817-9824

    DOI: 10.1021/acs.analchem.5b02354
    ISSN: 0003-2700
  • Isothermal DNA amplification strategies for duplex microorganism detection

    Santiago-Felipe, Sara; Tortajada-Genaro, Luis Antonio; Morais, Sergi; Puchades, Rosa; Maquieira, Angel

    (2015). Article

    Food Chemistry.

    A valid soln. for micro-​anal. systems is the selection of a compatible amplification reaction with a simple, highly-​integrated efficient design that allows the detection of multiple genomic targets. Two approaches under isothermal conditions are presented: recombinase polymerase amplification (RPA) and multiple displacement amplification (MDA)​. Both methods were applied to a duplex assay specific for Salmonella spp. and Cronobacter spp., with excellent amplification yields (0.2-​8.6 · 108 fold)​. The proposed approaches were successfully compared to conventional PCR and tested for the milk sample anal. as a microarray format on a compact disk (support and driver)​. Satisfactory...

    A valid soln. for micro-​anal. systems is the selection of a compatible amplification reaction with a simple, highly-​integrated efficient design that allows the detection of multiple genomic targets. Two approaches under isothermal conditions are presented: recombinase polymerase amplification (RPA) and multiple displacement amplification (MDA)​. Both methods were applied to a duplex assay specific for Salmonella spp. and Cronobacter spp., with excellent amplification yields (0.2-​8.6 · 108 fold)​. The proposed approaches were successfully compared to conventional PCR and tested for the milk sample anal. as a microarray format on a compact disk (support and driver)​. Satisfactory results were obtained in terms of resistance to inhibition, selectivity, sensitivity (101-​102 CFU​/mL) and reproducibility (below 12.5​%)​. The methods studied are efficient and cost-​effective, with a high potential to automate microorganisms detection by integrated anal. systems working at a const. low temp.

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    Volume 174
    Pages 509-515

    DOI: 10.1016/j.foodchem.2014.11.080
    ISSN: 0308-8146
  • Dual-​Polarization Interferometry: A Novel Technique To Light up the nanomolecular World

    Escorihuela, Jorge; Gonzalez-Martinez, Miguel Angel; Lopez-Paz, Jose Luis; Puchades, Rosa; Maquieira, Angel; Gimenez-Romero, David

    (2015). Article

    Chemical Reviews. Num.1

    A review. The topics include: general principles, measurement, comparison and combination with other methods, bioreceptor immobilization and applications.

    Volume 115
    Issue 1
    Pages 265-294

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    DOI: 10.1021/cr5002063
    ISSN: 0009-2665
  • Synthesis and evaluation of the chromo-fluorogenic recognition ability of imidazoquinoline derivatives toward ions

    Marín-Hernández, C., Santos-Figueroa, L. E., El Sayed, S., Pardo, T., Raposo, M. M. M., Batista, R. M., Costa, P.G. S., Martínez‐Mañez, R., Sancenón, F.

    (2015). Article

    Dyes and Pigments, vol. 122, p. 50-58.

     

    DOI: 10.1016/j.dyepig.2015.06.011
  • Antifungal effect of essential oil components against Aspergillus niger when loaded into silica mesoporous supports

    Bernardos, A., Marina, T., Žáček, P., Pérez‐Esteve, É., Martínez‐Mañez, R., Lhotka, M., Kouřimská, L., Pulkrábek, Josef., Klouček, P.

    (2015). Article

    Journal of the Science of Food and Agriculture, vol. 95, no 14, p. 2824-2831. 
     

    DOI: 10.1002/jsfa.7022