At present, the central line of the laboratory is an spectrometer of En la actualidad, el eje central del laboratorio es un espectrómetro de Nuclear Magnetic Resonance (NMR) of 14,1 Tesla (600 MHz) equipped with 4 measurement probes. These probes allow to obtain NMR spectra in a wide variety of samples which include biofluids (blood serum, plasma, urine, cerebrospinal fluid, tears, cell culture medium, etc.,) tissues (tumor biopsies, resections, liver punctures among others), cell lines (umbilical cords, hepatocytes).
This equipment is a key element for the development of the Metabolomics area with special emphasis on biomedical applications. Some of the available probes allow to work with very small amounts of sample (4-5 tissue micrograms and up to 10 microlitres of liquid).
On the other hand the equipment has a microimage probe with 3 different coils which can obtain Magnetic Resonance Images in high-resolution small samples and with the possibility to obtain a valuable molecular information.
- HR-MAS Probe; it allows to obtain the metabolic tissue profile (biosies, resections, punctures) and semisolid (cell cultures). The probe allows to work with quantities of sample between 5 and 25 µg.
- Probe of 5 mm; Conventional probe used for obtaining metabolic spectra in liquid samples including all types of biofluids. The quantity of sample used usually is 500 µL although depending on the initial concentration dilutions for inferior volumes can be obtained.
- Probe of 1 mm;very suitable for small sample volumes, we can work with numbers 10-20 µL. For the measurement, the sample is prepared in a capillary of 1 mm diameter. The resolution and quality of the obtained spectrum is comparable to conventional probe. Ideal for the study of animal models where sample availability is very limited.
- Microimage Probe; equipped with 3 different coils it allows us to obtain magnetic resonance images of a very high quality for samples up to 1cm2 (biopsies, resections, cardiac tissue, biomaterials).