| External users | Internal users | |
| Filamentous fungi strain identification by molecular and phenotypical methods | 299,70 € | 239,76 € |
| Yeast strain or arbuscular mycorrhizal fungi identification by molecular methods | 290,01 € | 232,01 € |
| Identification using partial 16S rRNA gene sequence | 208,78 € | 167,02 € |
| Identification using almost complete (>1400 bp) 16S rRNA gene sequence | 290,01 € | 232,01 € |
| Identification of strains by MALDI-TOF MS Biotyper | 39 € | 31,20 € |
| MSPs acquisition | 107,73 € | 107,73 € |
| MALDI-TOF MS cluster analysis | 53,87 € | 53,87 € |
| Protein extraction for MALDI-TOF MS | 15 € | 15 € |
| Prokaryotic genome (including checking by 16S rRNA gene sequencing). Each strain, minimum 6 strains (less than 6 strains ask for fees) | 678,38 € |
| Genomic analysis of a prokaryotic strain (assembly and annotation) | 339,24 € |
| Genomic analysis of an eukaryotic strain until 40 Mb (including checking by ITS region sequencing). Each strain | 1.185,03 € |
| G+C mol% content | 150,71 € |
| Genome comparison (up to 3 references) | 179,65 € |
| Cellular fatty acid analysis (MIDI) | 73,15 € |
| Phenotypic characterisation through API® 20A, 20E, 20NE, CANDIDA, CORYNE, LISTERIA, rapid ID 32STAPH or rapid ID 32STREP (minimun 5 strains) | 85 € |
| Phenotypic characterisation through API® 50CHB, 50CHE and 50CHL (minimun 5 strains) | 100 € |
| Morphological and physiological study (filamentous fungi) | 174,09 € |
| Morphological study of prokaryotes (cellular morphology, Gram, KOH, catalase, oxidase) | 58,7 € |
| Flagellar staining | 61,51 € |
|
Others (PHB detection, agarase activity, etc.). Minimum 4 strains From the fifth strain, each strain |
116,03 € 17,45 € |
| External users | Internal users | |
| Genomic region sequencing (recA, gyrB, ftsZ, pyrH, rpoD, mreB, ITS region, beta-Tubulin, TEF1, ACT1, etc) | 139,29 € | 111,44 € |
| Phylogenetic analysis from a genomic region | 710,05 € | 568,04 € |
|
Obtainment of genetic profiles (RAPDs, SSR...), every two strains From the third strain more, each strain From the second primer, every two strains From the third strain or more for each primer |
278,48 € 92,86 € 22,95 € 5,82 € |
278,48 € 92,86 € 22,95 € 5,82 € |
| Freeze-dried by request* | |
|
Minimum order 10 ampoules per strains |
143,93 € |
|
Additional ampoules |
10,34 € |
| Use of the freeze-dryer | 124,43 € |
| Biological material custody | |
|
In liquid N2 (per year and shared box) |
102,6 € |
| In liquid N2 (per year and complete box) | 205,2 € |
| Liquid N2 manipulation (insert or remove vials) | 56,02 € |
|
In -80ºC (each criovial box up to 2 ml and year) |
128,25 € |
|
In -80ºC manipulation (insert or remove vials) |
28,01 € |
| DNA extraction (5 µg) | 69,59 € |
| (50 µg) | 290,01 € |
| Isolation and purification | |
|
Isolation from a mixed culture plate, each plate |
58,07 € |
|
Isolation of arbuscular mycorrhizal fungi |
110,96 € |
| Plate counts in general media* | 114,84 € |
| Freeze-dried ampoule opening | 94,27 € |
| Issuance of certificates | 52,25 € |
| Issuance of reports | 116,03 € |
*Plate counts on specific media, ask for fees. Plate counts of arbuscular mycorrhizal fungi are performed in three independent assays and the fee will be applied for each replicate.
