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The services offered by the Proteomics unit of the SCSIE fall into the following types of services:

  • Preparation of the sample
    • Protein extraction: total proteome
    • Precipitation and concentration
    • Desalting and concentration by means of Zip-Tip (C18)
  • Protein electrophoresis on polyacrylamide gels
    • One-dimension electrophoresis 1D_SDS_PAGE with Coomassie blue, silver compatible or fluorescence staining.
    • Two-dimension electrophoresis with Coomassie blue stain, compatible silver or fluorescence.
  • Molecular mass determination
    • Determination of molecular mass: MS_MALDI_TOF
  • Protein identification
    • Protein identification: peptide print MALDI_TOF_TOF
    • Protein identification: LC_MSMS: high resolution (h)
    • Protein identification: 2D LC_MSMS
  • Protein characterisation
    • Post-translational modification analysis: phosphorylation
    • De Novo sequencing at peptic level
  • Differential proteomics
    • Analysis of diferencial expression in label-free:SWATH
  • Directed quantitative proteomics
    • Absolute quantification: SRM
  • Data analysis
    • Data analysis
    • Creation and/or adaptation of databases
    • Additional research in databases