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The services offered by the Proteomics unit of the SCSIE fall into the following types of services:

  • Preparation of the sample
    • Protein extraction: total proteome
    • Precipitation and concentration
  • Protein electrophoresis on polyacrylamide gels
    • One-dimension electrophoresis 1D_SDS_PAGE with Coomassie blue, silver compatible or fluorescence staining.
  • Molecular mass determination
    • Determination of molecular mass: MS_MALDI_TOF_TOF
  • Protein identification
    • Protein identification: LC_MSMS: high resolution (h)
  • Protein characterisation
    • Post-translational modification analysis: phosphorylation
  • Differential proteomics
    • Analysis of diferencial expression in label-free: DIA
  • Directed quantitative proteomics
    • Absolute quantification: SRM
  • Molecular image analysis
    • MALDI Imaging
  • Data analysis
    • Data analysis
    • Creation and/or adaptation of databases
    • Additional research in databases