Bacteriology of plants and Lichens. Biotechnological applications - BACPLANT

Reference of the Group:

GIUV2015-219

 
Description of research activity:
Laboratory authorised to work with quarantine plant pathogenic bacteria (biosafety level 2 conditions). Lines of research: Characterisation and diagnosis of plant pathogenic bacteria. Description: Conventional and molecular diagnosis, characterisation and identification of plant pathogenic bacteria (phytopathogens). Molecular typing and epidemiology. Activities: Use of rapid and specific molecular methods for the detection and diagnosis of plant pathogenic bacteria (quarantine bacteria such as Erwinia amylovora and Ralstonia solanacearum and other plant pathogens in general such as Lonsdalea quercina, Brenneria spp. etc.).Molecular epidemiology studies to determine intraspecific variability and pathways of introduction/dissemination of bacterial pathogens. Virulence and/or pathogenicity tests with different phytopathogenic bacteria. Survival strategies of plant pathogenic bacteria. Description: Bacterial survival strategies in different environments [survival in oligotrophic conditions, viable non-culturable state (VNC) and inducing factors], reservoirs and transmission pathways. Recovery of bacteria in VNC state. Gene expression and obtaining mutants in bacterial genes...Laboratory authorised to work with quarantine plant pathogenic bacteria (biosafety level 2 conditions). Lines of research: Characterisation and diagnosis of plant pathogenic bacteria. Description: Conventional and molecular diagnosis, characterisation and identification of plant pathogenic bacteria (phytopathogens). Molecular typing and epidemiology. Activities: Use of rapid and specific molecular methods for the detection and diagnosis of plant pathogenic bacteria (quarantine bacteria such as Erwinia amylovora and Ralstonia solanacearum and other plant pathogens in general such as Lonsdalea quercina, Brenneria spp. etc.).Molecular epidemiology studies to determine intraspecific variability and pathways of introduction/dissemination of bacterial pathogens. Virulence and/or pathogenicity tests with different phytopathogenic bacteria. Survival strategies of plant pathogenic bacteria. Description: Bacterial survival strategies in different environments [survival in oligotrophic conditions, viable non-culturable state (VNC) and inducing factors], reservoirs and transmission pathways. Recovery of bacteria in VNC state. Gene expression and obtaining mutants in bacterial genes of interest. Activities: Studies of survival of phytopathogenic bacteria in natural water microcosms and/or other environmental reservoirs.Studies of reservoirs of phytopathogenic bacteria and transmission pathways. Studies of factors inducing entry into the VNC state. Studies of recovery and/or resuscitation of VNC cells. Studies on the effect of biotic and abiotic factors on the survival and pathogenicity of phytopathogenic bacteria. Studies of the effect of different stress factors on the expression of genes related to the survival and/or virulence of phytopathogenic bacteria. Obtaining mutants in genes related to the survival and/or virulence of phytopathogenic bacteria. Studies of the survival and virulence of bacterial mutants of plant pathogens Characterisation of bacterial mutants of plant pathogens by microbiological, microscopic, molecular and/or omic techniques. Biotechnological applications of environmental micro-organisms. Description: Isolation and characterisation of environmental micro-organisms of biotechnological interest: bacteriophages, micro-organisms producing antimicrobial compounds, siderophores, degrading micro-organisms. Biological control of bacteriosis in plants. Activities: Isolation and characterisation of specific bacteriophages of phytopathogenic bacteria. Survival studies of specific bacteriophages of phytopathogenic bacteria. Isolation and characterisation of microorganisms producing antimicrobials and other metabolites of interest. Biological control of bacterial plant diseases. Isolation and characterisation of hydrocarbon-degrading environmental microorganisms. Study of possible applications in bioremediation. Bacteriology of lichens. Biotechnological applications. Description: Isolation and characterisation of lichen-associated bacteria: diversity, contribution to lichen symbiosis, and biotechnological applications. Activities: Isolation of bacteria associated with lichens. Obtaining lichen extracts. Optimisation of the recovery of lichen bacteria. Diversity of lichen bacteria. Role of lichen-associated bacteria in lichen symbiosis. Biotechnological characterisation of lichen bacteria and their applications: plant growth promoting microorganisms, waste degraders, producers of new polymers, pigments, etc.
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Scientific-technical goals:
  • 1. Caracterizacion y diagnostico de bacterias patogenas de plantas
  • 2. Estudias estrategias de supervivencia de bacterias patogenas de plantas con objeto de prevenir y/o controlar las enfermedades que causan
  • 3. Evaluar las posibles aplicaciones biotecnologicas de microorganismos ambientales en control biologico y/o biorremediacion
  • 4. Estudiar la composicion, diversidad y potencial biotecnologico de las comunidades bacterianas asociadas a los liquenes
 
Research lines:
  • Characterisation and diagnosis of plants pathogenic bacteria.Diagnosis, characterisation, and conventional and molecular identification of plants phatogenic bacteria (phytopathogen). Molecular typing and epidemiology.
  • Survival strategies of plants phatogenic bacteria.Bacterial survival strategies in different environments [survival in oligotrophic conditions, viable non-culturable state (VNC) and inducer factors], reservoirs and transmission routes. Recovery of bacteria in VNC state. Gene expression and obtaining mutants in bacterial genes.
  • Biotechnological applications of environmental microorganisms.Isolation and characterisation of environmental microorganisms of biotechnological interest: bacteriophages, microorganisms producing antimicrobial compounds, siderophores, degrader microorganisms. Biological control of bacteriosis in plants.
  • Lichen bacteriology. Biotechnological applications.Isolation and characterisation of bacteria associated to lichen: diversity, contribution to lichen symbiosis and biotechnological applications.
 
Group members:
Name Nature of participation Entity Description
ELENA GONZALEZ BIOSCADirectorUniversitat de València
Research team
MARÍA BELEN ÁLVAREZ ORTEGACollaboratorInstituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentariolaboratory head
 
CNAE:
  • -
  • -
 
Associated structure:
  • Microbiology and Ecology
 
Keywords:
  • Diagnóstico, Caracterización fenotípica, Caracterización genotípica, Detección molecular, PCR, Identificación molecular, epidemiología molecular
  • Caracterización,Epidemiología,Estrategias de supervivencia,Supervivencia en inanición,Estado VNC, Recuperación y/o resucitación de células VNC
  • Caracterización,Identificación,Tipificación molecular,Microscopía Electrónica, Microorganismos de interés biotecnológico, Fagos, Control biológico
  • Caracterización,Identificación,Microscopía, Microorganismos de interés biotecnológico,Simbiosis, Diversidad bacteriana. Control biológico