ZENOTOF 7600 is a high resolution MSMS spectrometrer that incorporates two essential novelties: on one hand, the trap (ZENO) that allows to increase the efficacy of transmission and cluster of ions, improving 5-20 times the obtained sensitivity on the traditional QTOF. The other great novelty of ZENOTOF is the incorporation of the EAD (Electron Activated Dissociation) fragmentation technology, a specific fragmentation system that allows to improve the capacity of elucidation of compounds. 

1) ULTRA HIGH PRESSURE LIQUID CHROMATOGRAPHY (UHPLC) SYSTEM

Minimum technical specifications of the system:

• Pump system:
• Two double high-precision piston pumps, with the ability to reach a working pressure of 1.300 bars.
• Flow from 0.0001 to 3 mL/min.
• Flow precision: ± 0.06% RSD.
• Flow accuracy: ± 1%
• Automatic cleaning of pistons.
• Leak detector for a pump.
• Minimum membrane degasser up to five canals. 
• pH operating range of 1-14.
• The accuracy in the composition of the solvent mixture, expressed as a concentration, is of ± 0.5%, what enables reproducible results.
• Gradient formation range from 0 to 100 % (in steps of 0,1 %)
• Mixer of high capacity for the gradient formation
• Column ovens:
  • Controlled thermostated column ovens, from – 10º C below the room temperature to 100º C.
  • Control temperature precision of  ±0,05º C.
  • Control temperature accuracy of ±0,5
  • Capacity up to 6 columns of 250 mm or 3 columnas of 300mm
  • Valve of high-pressure for an automatic exchange of two columns
• Automatic injector
  • Capacity of 162 samples placed in vials of 1,5 mL
  • Injection volume from 0.1μL to 50 μL.
  • Cooling system of samples from 4ºC to 45ºC.
  • Maximum cross-contamination (carry-over) of 0.0015%.
  •  Injection volume accuracy ±1%
  • Linealidad ≥ 0,9999
  • Precisión del volumen de inyección. RSD ≤ 0.25%
  • Linearity ≥ 0,9999
  • Maximum work pressure up to 1300 bar.
  • Minimum injection time. 14 seconds.
  • Supplied with leak sensor

2) SPECTROMETER SYSTEM OF HIGH RESOLUTION MASS AND EXACT MASS, QTOF

Minimum technical specifications of the system:

• Sensitivity in TOF/MS: in ESI positive mode for an injection of 1 pg of reserpine in column, produces a S/N > 2500 relation
• Sensitivity in MS/MS: in ESI positive mode for an injection of 50 fg of reserpine in column, produces a S/N > 400 relation
• Sensitivity in TOF/MS: With the Twin Sprayer sounding line, an injection of 1 pg of reserpine in column, produces a S/N > 750 relation
• Scanning speed in MS/MS: From133 Hz (133 spectrums MS/MS per second)
• MS/MS duty cycle: ≥ 90% for acquisition in TOF MS/MS
• MS resolution: more than 42,000 (FWHM) m/z 956.
• Scanning modes: Fullscan TOF MS, Full scan TOF MS/MS, MRMHR, SWATH (DIA), IDA (DDA)
• Internal mass accuracy: less than 0.2 ppm of absolute error, proved in MS/MS in a piece of [Glu]1-Fibrinopeptide B
• External mass accuracy: less than 2 ppm RMS over 12 working hours of LC-MS
• Linear dynamic range: the best of 5 orders of magnitude without signal loss.
• Mass range: In the TOF, 40 kDa, in Precursor Ion, 5-2.250 m/z (5-40.000 m/z)
• Working flow range: From 5 μL/min to 3 mL/min
• Advanced modes of data acquisition: Full Scan TOF MS, Full Scan TOF MS/MS, MRMHR, Data Dependent Adquisition (DDA) and Data Independent Adquisition (DIA)
• Fragmentation modes: CID collision cell (Collision Induced Dissociation) and EAD cell (Electron Activated Dissociation)